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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Dual-Labeled Probe °ËÃâ > One Step PrimeScript¢â RT-PCR Kit (Perfect Real Time)
Probe¸¦ »ç¿ëÇÑ °í°¨µµ °ËÃâ 1 step RT qPCR

One Step PrimeScript¢â RT-PCR Kit (Perfect Real Time)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
RR064A
One Step PrimeScript¢â RT-PCR Kit (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
(50 §¡ ¹ÝÀÀ°è)
344,800¿ø 
431,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR064A_e.v2202Da.pdf
Takara
RR064B
One Step PrimeScript¢â RT-PCR Kit (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ȸ (RR064A x 5)
1,940,000¿ø 

  • ¹ÙÀÌ·¯½º µî ¹Ì·® RNA °ËÃâÀ» ½Å¼ÓÇÏ°í Á¤È®ÇÏ°Ô ÁøÇà °¡´É
  • Real Time PCR¿¡ ÃÖÀûÈ­µÈ 2X ³óµµÀÇ premix ¹öÆÛ¸¦ »ç¿ëÇÑ °£´ÜÇÑ ½ÇÇè
  • Premix ÇüÅ·Π»ç¿ëÀÌ ´õ¿í Æí¸®ÇØÁö°í PCR inhibitor ÀúÇ×¼ºÀÌ Çâ»óµÈ One Step PrimeScript™ III RT-qPCR Mix (Code RR600A)À» È®ÀÎÇϼ¼¿ä.
Á¦Ç°¼³¸í
One Step PrimeScript™ RT-PCR Kit (Perfect Real Time)Àº dual-labeled probeÀ» »ç¿ëÇÑ 1 step Real Time RT-PCR KitÀÌ´Ù. ÇϳªÀÇ Æ©ºê ¾È¿¡¼­ ¿ªÀü»ç ¹ÝÀÀ (reverse transcription)ºÎÅÍ Real Time PCR±îÁö ¿¬¼ÓÀûÀ¸·Î ¹ÝÀÀÀÌ ÀÌ·ç¾îÁö±â¿¡ Á¶ÀÛÀÌ °£ÆíÇÏ°í ¿À¿° À§ÇèÀÌ ³·´Ù.
PCRÀÇ ÁõÆø »ê¹°À» ½Ç½Ã°£À¸·Î °ËÃâÇϱ⠶§¹®¿¡ Àü±â¿µµ¿ µîÀ¸·Î º°µµ·Î È®ÀÎÇÒ ÇÊ¿ä°¡ ¾øÀ¸¸ç, RNA ¹ÙÀÌ·¯½º µîÀÇ ¹Ì·® »ùÇÃÀÇ °ËÃâ¿¡ À¯¸®ÇÏ´Ù.
º» Á¦Ç°Àº ´ÙÄ«¶óÀÇ °í¼º´É High fidelity RTase PrimeScript™ RTase¿Í ÁõÆø È¿À²ÀÌ ¶Ù¾î³­ °í¼º´É PCR È¿¼Ò TaKaRa Ex Taq® HS¸¦ »ç¿ëÇØ 1 step RT-PCR¿¡ ÃÖÀûÈ­µÇ¾î ³ôÀº ƯÀ̼º°ú ÁõÆøÈ¿À²·Î ¾ÈÁ¤ÀûÀÎ rt-qPCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
Àû¿ë °¡´ÉÇÑ PCR ±â±â
ÀÌ Á¦Ç°Àº ¾Æ·¡ÀÇ °¢Á¾ PCR±â±â¿ÍÀÇ È£È¯¼ºÀÌ È®ÀεǾú´Ù.
  - CronoSTAR 96 Real-Time PCR System (Code 640231 ¿Ü)
  - Thermal Cycler Dice Real Time System III (Code TP950 ¿Ü)
  - Appied Biosystems 7300/7500/7500 Fast Real-Time PCR System
  - StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)
  - LightCycler (Roche Diagnostics»ç)
  - Smart Cycler System/Smart Cycler II System (Cepheid»ç)µî
ÀÌ¿Ü¿¡µµ ÀϹÝÀûÀÎ qPCR ±â±â¿Í ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50 §¡ ¹ÝÀÀ¾×, 100ȸºÐ)

2x One Step RT-PCR  Buffer III*1

3x 840 §¡

TaKaRa Ex Taq HS (5 U/§¡)

100§¡

PrimeScript RT enzyme Mix II*2

100§¡

RNase Free dH2O

2x 1.25 §¢

ROX Reference Dye (50×conc)*3

100 §¡

ROX Reference Dye II (50×conc)*3

100 §¡

*1 dNTP Mixture, Mg2+°¡ Æ÷ÇԵǾî ÀÖ´Ù.
*2 RNase inhibitor¸¦ Æ÷ÇԵǾî ÀÖ´Ù.
*3 Applied BiosystemÀÇ qPCR ±â±â µî¿¡¼­ well°£ÀÇ ½ÅÈ£¸¦ º¸Á¤Çϴµ¥ »ç¿ëÇÑ´Ù.
  • ROX reference dye¸¦ »ç¿ëÇÏ´Â qPCR±â±â
    - Applied Biosystems 7300 Real-Time PCR System(Thermo Fisher Scientific)
    - StepOnePlus Real-Time PCR System(Thermo Fisher Scientific)
  • ROX reference dye II¸¦ »ç¿ëÇÏ´Â qPCR ±â±â
    - Applied Biosystems 7500/7500 Fast Real-Time PCR System(Thermo Fisher Scientific)
  • ROX reference dye ÷°¡°¡ ÇÊ¿ä ¾ø´Â ±â±â
    - CronoSTAR 96 Real-Time PCR System(Code 640231 ¿Ü)
    - Thermal Cycler Dice Real Time System½Ã¸®Áî (Code TP950 ¿Ü)
    - LightCycler (Roche Diagnostics)
    ÀÚ¼¼ÇÑ ROX reference dye »ç¿ë¿¡ ´ëÇÑ ³»¿ëÀº °¢ ±â±âÀÇ Á¦Á¶»ç¿¡ ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
º¸Á¸ - 20¡É
»ç¿ë»óÀÇ ÁÖÀÇ
ÀÌ Á¦Ç°ÀÇ ¿ªÀü»ç ¹ÝÀÀÀº PCR¿ë specific primer¸¦ »ç¿ëÇÑ´Ù. º°µµÀÇ random primer, oligo dT primer´Â »ç¿ëÇÒ ¼ö ¾ø´Ù.
°ü·ÃÁ¦Ç°
- gDNA Á¦°Å columnÀÌ Æ÷ÇÔµÈ RNA ÃßÃâ Kit, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
- Virus À¯·¡ RNA ȤÀº DNA Á¤Á¦ Kit, TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Code 9766A)
- AGPC¹ýÀ» È°¿ëÇÑ RNA Á¤Á¦ Kit, RNAiso Plus (Code 9109 ¿Ü)
- Premix ÇüÅ·Π»ç¿ëÀÌ Æí¸®ÇÏ°í PCR inhibitor ÀúÇ×¼ºÀÌ Çâ»óµÈ 1step RT qPCR Kit, One Step PrimeScript™ III RT-qPCR Mix (Code RR600A)

Keyword : Taqman,probe,dual labeled,taqman probe,qPCR,Real time pcr

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