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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > [Legacy] NGS Products > (Legacy) SMARTer¢ç Stranded Total RNA-Seq Kit - Pico Input Mammalian
Picogram ¼öÁØ Total RNA·ÎºÎÅÍ Stranded RNA-Seq

(Legacy) SMARTer¢ç Stranded Total RNA-Seq Kit - Pico Input Mammalian

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* ´õ ³ôÀº È¿À²À» º¸À¯ÇÑ ¾÷±×·¹À̵å Á¦Ç°, SMARTer¢ç Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian »ç¿ëÀ» ÃßõÇÕ´Ï´Ù.

  • Picogram ¼öÁØÀÇ ±Ø¼Ò·® »ùÇà Àû¿ë: 250 pg - 10 ng total RNA (Human, mouse, rat)
  • Versatile: FFPE, LCM »ùÇÃÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ Á¾·ùÀÇ Sample Àû¿ë °¡´É
  • Stranded information: ¸Å¿ì ³ôÀº Á¤È®¼ºÀ¸·Î transcript strand information À¯Áö
  • Fast, streamlined protocol: 5½Ã°£ ³» NGS Library Á¦ÀÛ ¿Ï·á
  • Seamless integration with Illumina sequencing

SMARTer Stranded Total RNA-Seq Kit - Pico Input MammlianÀº Picogram ¼öÁØÀÇ total RNA (250 pg - 10ng)·ÎºÎÅÍ Illumina sequencing library¸¦ È¿À²ÀûÀ¸·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ high-quality, partially degraded RNA »Ó¸¸ ¾Æ´Ï¶ó low quality total RNA (FFPE ¶Ç´Â LCM »ùÇà µî)·ÎºÎÅ͵µ NGS library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù. º» Á¦Ç°Àº strand informationÀ» Á¤È®ÇÏ°Ô À¯ÁöÇÒ ¼ö ÀÖÀ» »Ó ¾Æ´Ï¶ó cDNA ÇÕ¼º ÀÌÈÄ º°µµÀÇ library preparation °úÁ¤ ¾øÀÌ ¿ªÀü»ç°úÁ¤°ú PCR ÁõÆø°úÁ¤¿¡¼­ Illumina sequencing ºÐ¼®¿ë index¿Í adaptor¸¦ ºÎÂøÇÒ ¼ö ÀÖ´Ù.
Ribosomal RNA (rRNA)´Â total RNAÀÇ ¾à 90%¸¦ Â÷ÁöÇÏ´Â RNA·Î½á NGS library Á¦ÀÛ Àü, rRNA¸¦ Á¦°Å(depletion)ÇÔÀ¸·Î½á Á¦ÀÛºñ¿ëÀ» Àý°¨ÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó mapping statistics ¶ÇÇÑ Çâ»ó½Ãų ¼ö ÀÖ´Ù. ÇÏÁö¸¸ ¸Å¿ì ±Ø¼Ò·®ÀÇ Total RNA¸¦ ÀÌ¿ëÇÒ °æ¿ì ÀϹÝÀûÀÎ ¹æ¹ýÀÇ rRNA depletionÀº NGS library Á¦Á¶ÇÏ´Â µ¥ ÇÊ¿äÇÑ ¾ç¿¡ ¹ÌÄ¡Áö ¸ø ÇÏ°Ô µÈ´Ù. º» Á¦Ç°¿¡ »ç¿ëµÈ ±â¼úÀº mammalian rRNA¿Í mitochondrial RNA ƯÀÌÀûÀÎ Probe¸¦ ÀÌ¿ëÇÏ¿© rRNA derived cDNA¸¦ Á¦°ÅÇÑ´Ù.
High-troughput versionÀÎ SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Code 635007)Àº 96°³ÀÇ index library Á¦ÀÛÀ» À§ÇÑ 12°³ÀÇ reverse primer¿Í 8°³ÀÇ forward primer¸¦ Æ÷ÇÔÇϰí ÀÖ´Ù.

Alternate kits for other input amounts:


±×¸² 1. SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian ¿ø¸®
This workflow allows users to generate Illumina-compatible libraries for RNA-seq experiments. After library preparation and purification, the total time from input RNA to RNA-seq library is approximately five hours.


±×¸² 2. Human tissue·ÎºÎÅÍ Àü»çü ºÐ¼® °á°ú¿Í exon mapping °á°ú
Distribution of reads in libraries prepared with the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian in the absence (-) or presence (+) of R-Probes, using 250 pg of hum an total RNA. Sequences corresponding to 1 million paired-end reads per library were analyzed.


±×¸² 3. FFPE, LCM »ùÇðú °°Àº degraded RNA·ÎºÎÅÍ capture °á°ú
Distribution of insert sizes are shown for libraries generated from 250 pg of high-quality (RIN 8) or highly degraded (RIN 2.5) human total RNA using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian with a 4 min shearing time (RIN 8) or with the alternate no-shearing protocol (RIN 2.5). RNA-seq libraries were sequenced on an Illumina MiSeq instrument in paired-end mode. The number of mapped fragments with any given insert size was normalized to the total number of fragments in the library. Fragments mapping to rRNA or the mitochondrial genome were excluded.


±×¸² 4. Ribosomal cDNA depletion¿¡ À¯¹«¿¡ µû¸¥ High reproducibility
Comparison of FPKMs from libraries generated with 250 pg human brain or heart total RNA with (+) or without (-) ribosomal cDNA depletion (i.e., with or without R-Probes, respectively) using the SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian. FPKM values are shown on a Log10 scale. Transcripts represented in only one library can be seen along the x- and y-axes of the scatter plots.
Àû¿ë
  • Robust NGS library generation that retains strand information
  • Use for RNA-seq on Illumina platforms
  • Captures coding and non-coding information from total mammalian RNA
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Code 635005)
  • SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian Components
  • Indexing Primer Set HT for Illumina - 12

SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Code 635006)
  • SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian Components
  • Indexing Primer Set HT for Illumina - 48A

SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Code 635007)
  • SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian Components
  • Indexing Primer Set HT for Illumina

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