Caspase Assay Kits

• The Caspase-9/6 Fluorescent Assay detects the shift in fluorescence of 7- amino-4-methoxy coumarin (AMC). Upon cleavage, AMC fluorescence can be measured using a 380-nm excitation filter and a 460-nm emission filter. Fluorescence detection is highly sensitive and can be used to measure even very small amounts of active caspase.
• The Caspase-3 and -8 Fluorescent Assays detect the shift in fluorescence emission of 7-amino-4-trifluoromethyl coumarin (AFC). After the substrate is cleaved by the protease, AFC emits a yellow-green fluorescence at 505 nm if excited at 400 nm.
• The Caspase-3 and -8 Colorimetric Assays measure the proteolytic cleavage of the chromophore p-nitroanilide (pNA) by caspase- 3 or -8. Liberated pNA can be monitored colorimetrically by absorbance at 405 nm.

Caspase Assay Plates

• The ApoAlert Caspase Profiling Plate (Figure 3), measures the activation of four different caspases―caspase-2, -3, -8, and -9―using a fluorometric method in a convenient 96- well plate format. Caspase profiling plates make it easy to profile caspase activities in a variety of cell lines after treatment with different apoptotic stimuli.
• The ApoAlert Caspase-3 Assay Plate provides a convenient way to monitor caspase-3 activity in up to 96 samples at once. No handling of caspase substrates is required, allowing for easy detection of caspase-3 activity in a variety of samples. Caspase-specific inhibitors are included for your control needs.

No Substrate Handling Required