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Ç×ü ¡¤ ELISA ¡¤ Signal
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Apoptosis
> In situ Apoptosis Detection Kit
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
In situ Apoptosis Detection Kit
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(ºÎ°¡¼¼º°µµ)
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Takara
MK500
in situ Apoptosis Detection Kit
20 ȸ
322,400¿ø
403,000¿ø
09.02 ~ 10.31
Takara
MK501
Labeling Safe Buffer
500 ¥ìl¡¿10
660,800¿ø
826,000¿ø
09.02 ~ 10.31
Takara
MK502
TdT Enzyme
50 ¥ìl¡¿10
304,800¿ø
381,000¿ø
09.02 ~ 10.31
Takara
MK503
Anti-FITC HRP Conjugate
1.5 ml¡¿5
279,200¿ø
349,000¿ø
09.02 ~ 10.31
Takara
MK504
Control Slides
2 Slides¡¿5
133,600¿ø
167,000¿ø
09.02 ~ 10.31
Takara
MK505
Permeabilisation Buffer
1.0 ml¡¿10
112,800¿ø
141,000¿ø
09.02 ~ 10.31
Product index
¸é¿ª°Ë»ç°ü·ÃÁ¦Ç°
TaKaRa DAB Substrate
TaKaRa POD Conjugate½Ã..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã Ç×..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã..
EIA Kit
Acrolein-Lysine Adduct..
Fibronectin EIA Kit
Human Albumin EIA Kit
Human IgG EIA Kit
Human Vitronectin EIA ..
Human/Pig Osteonectin ..
Laminin(LN) EIA Kit
Mouse Albumin EIA Kit
Mouse Gla-Osteocalcin ..
Osteocalcin EIA Kit
Peptide Coating Kit
Pig Osteocalcin EIA Ki..
Procollagen Type I C-p..
Rat Gla-Osteocalcin Hi..
Rat Heme Oxygenase-1 E..
RetroNectin¢ç Á¤·® kit
Universal Tyrosine Kin..
Wash and Stop Solution..
Osteo¤ýAdipo assay
AdipoRed Assay Reagent
Osteoblast-Inducer Rea..
OsteoImage Mineralizat..
PDELight HTS cAMP Phos..
PPiLight Inorganic Pyr..
TRACP & ALP Assay Kit
TRACP & ALP double-sta..
¼¼Æ÷Áõ½Ä ¡¤ ¼¼Æ÷ÀåÇØ ÃøÁ¤
Premix WST-1 Cell Prol..
Luminescent Assay Acce..
Premix WST-1 Cell Prol..
ToxiLight BioAssay Kit
ViaLight ½Ã¸®Áî
È¿¼ÒÈ°¼º ÃøÁ¤ ¡¤ °ËÃâ¿ë Ki..
Heparan Degrading Enzy..
Apoptosis
ApoAlert Annexin V-FIT..
ApoAlert Caspase Assay..
ApoAlert pDsRed2-Bid V..
ApopLadder Ex¢â
ApoPrimer Set (Bcl-2 f..
In situ Apoptosis Dete..
Signal Transduction
Dominant-Negative Vect..
ExpressHyb Hybridizati..
Signal Àü´Þ °æ·Î Profi..
Ç×ü
Ç×ü ±³Â÷ ¹ÝÀÀ À϶÷Ç¥
Ç×ü ¿ëµµ À϶÷Ç¥
Anti-Albumin
Anti-Bovine Osteocalci..
Anti-CD3 mAb GMP grade
Anti-Chicken N-cadheri..
Anti-Heme Oxygenase-1
Anti-Human E-cadherin
Anti-Human Fibronectin
Anti-Human Influenza V..
Anti-Human Laminin
Anti-Human N-cadherin
Anti-Human Osteocalcin
Anti-Human P-cadherin
Anti-Human Platelet GM..
Anti-Human Procollagen..
Anti-Human Vitronectin
Anti-Human von Willebr..
Anti-Insulin C
Anti-Mouse E-cadherin
Anti-Mouse Osteocalcin
Anti-Mouse P-cadherin
Anti-Osteonectin/SPARC
Anti-ProS2
Anti-Rat Collagen type..
Anti-Rat Osteocalcin
Monoclonal 2nd antibod..
Polyclonal Anti-Dentin..
Polyclonal Anti-Glucag..
Polyclonal Anti-Human ..
Polyclonal Anti-Mouse ..
Polyclonal Anti-N-cadh..
Polyclonal Anti-Rat Bo..
³»¿ë (20ȸ)
1. Labeling Safe Buffer
500 §¡¡¿2 vials
2. TdT Enzyme
50 §¡¡¿2 vials
3. Anti-FITC HRP Conjugate
4. Control Slides
2 slides
5. Permeabilisation Buffer
1.0 §¢¡¿2 vials
*°¢ ±¸¼ºÇ° º°µµÆǸŠ°¡´É
º¸Á¸
1, 2, 5: -20¡É
3: 4¡É (¼ö¼Û ½Ã´Â -20¡É. Çѹø À¶ÇØÇÑ ÈÄ¿¡´Â 4¡É¿¡ º¸Á¸)
4: ½Ç¿Â (¼ö¼Û ½Ã´Â -20¡É)
Á¦Ç°¼³¸í
º» Å°Æ®´Â apoptosis¸¦ ÀÏÀ¸Å°´Â ¼¼Æ÷ÀÇ Æ¯Â¡ Áß ÇϳªÀÎ ´ÜÆíÈµÈ chromatin DNA¸¦ ¸»´ÜÇ¥½Ä¹ý¿¡ ÀÇÇØ Á¶Á÷ÈÇÐÀûÀ¸·Î °ËÃâÇÏ´Â Å°Æ®ÀÌ´Ù. In situ¿¡¼ Ç¥½ÄÇÙ»êÀÇ ÇÔÀÔ (incorporation)À¸·Î apoptosisÀÇ Á¶Á÷ÇÐÀû ºÐÆ÷³ª °¢°¢ÀÇ ¼¼Æ÷¸¦ È¿À²ÀûÀ¸·Î °ËÃâÇÏ´Â TUNEL (TdT-mediated dUTP-biotin nick end labeling)¹ýÀ» ÀÌ¿ëÇÏ¿© Á¶Á÷´ÜÆí, °íÁ¤¼¼Æ÷ µîÀÇ ¾î¶² ½Ã·á¿¡µµ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç Çü±¤Çö¹Ì°æ, ±¤ÇÐÇö¹Ì°æ, flow cytometry·Îµµ °üÂûÀÌ °¡´ÉÇÏ´Ù.
º» Å°Æ®¿¡´Â ¸é¿ª¹ÝÀÀ¿ë ±âÁúÀ̳ª ¿°»ö¿ë ½Ã¾àÀº Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù. Control Slide´Â ·¡Æ®(rat) À¯¼±Á¶Á÷ÀÇ ÆĶóÇÉ (paraffin) Æ÷¸Å´ÜÆíÀ̸ç, Positive Control Slide·Î »ç¿ëÇÒ ¶§´Â ÆĶóÇÉÀ» Á¦°ÅÇÑ ÈÄ¿¡ ÷ºÎÇÑ »ç¿ë¼³¸í¼ÀÇ ¡¸Á¶ÀÛ¼ø¼¡¹ (ÆĶóÇÉ Æ÷¸Å´ÜÆíÀÇ °æ¿ì)¿¡ µû¶ó »ç¿ëÇÑ´Ù.
Å»ÆĶóÇÉ°ú Proteinase K¸¦ ó¸®ÇÑ ÈÄ¿¡´Â °üÂû¹æ¹ý¿¡ µû¶ó ÀûÀýÇÑ ÇÁ·ÎÅäÄÝ·Î Á¶ÀÛÇÑ´Ù.
Å°Æ®ÀÇ component´Â ´ÜÇ° (Bulk)À¸·Îµµ ÆǸÅÇÑ´Ù.
ÃøÁ¤¿ø¸®
TUNEL¹ýÀº apoptosis¸¦ ÀÏÀ¸Å² ¼¼Æ÷¿¡¼ ´ÜÆíÈµÈ DNAÀÇ À¯¸® 3¡¯-OH ¸»´ÜÀ» terminal transferase (TdT)·Î fluorescein-dUTP¸¦ °íÈ¿À²ÀûÀ̸鼵µ ƯÀÌÀûÀ¸·Î Ç¥½ÄÇÑ ÈÄ Çü±¤Çö¹Ì°æÀ̳ª flow cytometry·Î °ËÃâÇÏ´Â ¹æ¹ýÀÌ´Ù. ÇÔÀÔµÈ fluoresceinÀº peroxidase Ç¥½Ä anti-fluorescein Ç×ü¸¦ ÀÌ¿ëÇÑ ¹ß»ö¹ÝÀÀÀ» ÅëÇÏ¿© ±¤ÇÐÇö¹Ì°æÀ¸·Îµµ °üÂûÇÒ ¼ö ÀÖ´Ù.
Keyword :
Apoptosis
ApoAlert Annexin V-FITC Apoptosis Kit
ApoAlert Caspase Assay Kit
ApoAlert pDsRed2-Bid Vector
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ DNA °ËÃâ
ApopLadder Ex¢â
Bcl-2 À¯ÀüÀÚ RT-PCR¿¡
ApoPrimer Set (Bcl-2 family)
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
In situ Apoptosis Detection Kit