´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Ç×ü ¡¤ ELISA ¡¤ Signal > EIA Kit > Wash and Stop Solution for ELISA without Sulfuric Acid
ELISA¿ë buffer ¼¼Æ®

Wash and Stop Solution for ELISA without Sulfuric Acid

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
MK021
Wash and Stop Solution for ELISA without Sulfuric Acid
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 well plate ¡¿ 5 plates
59,200¿ø 
74,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
MK021_DS.v1811Da.pdf

Á¦Ç°¼³¸í
Wash and Stop Solution for ELISA without Sulfuric Acid´Â ´ÙÄ«¶ó EIA kit¸¦ ÀÌ¿ëÇÒ ¶§ ÇÊ¿äÇÑ washing solution (10 ¡¿ PBS, Tween20)°ú peroxidase substrate ¹ß»öÀ» ¸ØÃߴµ¥ »ç¿ëÇÏ´Â ¹ÝÀÀÁ¤Áö¾× (stop solution)À¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
º» Á¦Ç°¿¡ Æ÷ÇÔµÈ ¹ÝÀÀÁ¤Áö¾×Àº Ȳ»ê (Sulfuric acid)À» Æ÷ÇÔÇÏ°í ÀÖÁö ¾ÊÁö¸¸, ±âÁ¸ÀÇ ¹ÝÀÀÁ¤Áö¾× (1N H2SO4)°ú °°Àº ¹æ¹ý, ¿ë·®À¸·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À¸·Î Á¶Á¦ÇÑ Washing solution ¹× ¹ÝÀÀÁ¤Áö¾×Àº °¢ EIA kit¿¡ ¾È³»µÈ ¹æ¹ý°ú ¿ë·®¿¡ µû¶ó »ç¿ëÇÑ´Ù.
³»¿ë

1.

10 ¡¿ PBS

50 §¢ ¡¿ 5

2.

Tween20

3 §¢

3.

Stop Solution

60 §¢

º¸Á¸ ½Ç¿Â
»ç¿ë¹æ¹ý
Wash buffer
  • PBS wash buffer
    : 10 ¡¿ PBS 1 bottle (50 §¢)À» ÃÖÁ¾ volume 500 §¢ÀÌ µÇµµ·Ï Áõ·ù¼ö·Î Èñ¼®ÇÑ´Ù.
    ÃæºÐÈ÷ ¼¯Àº ÈÄ wash buffer·Î »ç¿ëÇÑ´Ù. (»ç¿ë½Ã Á¶Á¦ÇÒ °Í)
  • PBST (PBS + 0.1% Tween20) wash buffer
    : 10 ¡¿ PBS 1 bottle (50 §¢)À» 450 §¢ÀÇ Áõ·ù¼ö¿¡ ¼¯¾î 1 ¡¿ PBS·Î Èñ¼®ÇÑ´Ù (ÃÖÁ¾ volume 500 §¢).
    Tween20 500 §¡¸¦ ÷°¡ÇÑ ÈÄ, ÃæºÐÈ÷ ¼¯¾î¼­ »ç¿ëÇÑ´Ù(»ç¿ë½Ã Á¶Á¦ÇÒ °Í).

¡¼ÁÖÀÇ¡½
¹Ýµå½Ã ÇÔ²² »ç¿ëÇÏ´Â EIA kitÀÇ ÇÁ·ÎÅäÄÝ¿¡¼­ ±ÇÀåÇÏ´Â wash buffer¿¡ µû¶ó PBS ¶Ç´Â PBST¸¦ ¼±ÅÃÇÏ¿© »ç¿ëÇÑ´Ù (EIA kit¿¡ µû¶ó »ç¿ëÇÏ´Â wash buffer°¡ ´Ù¸¦ ¼ö ÀÖÀ½).

Stop Solution
  • Stop SolutionÀ» ±×´ë·Î »ç¿ëÇÑ´Ù.

¡¼ÁÖÀÇ¡½
Stop SolutionÀº Á¡µµ°¡ ³ôÀº ¿ë¾×À̹ǷΠ÷°¡ ÈÄ plate mixer·Î ÃæºÐÈ÷ È¥ÇÕÇÑ´Ù.
»ç¿ë»óÀÇÁÖÀÇ
  • 10 ¡¿ PBS´Â º¸°ü¿Âµµ°¡ ³·À» °æ¿ì ÇϾá ħÀü¹°ÀÌ »ý±æ ¼ö ÀÖ´Ù. ÀÌ °æ¿ì µû¶æÇÏ°Ô ÇÏ¿© ¿ÏÀüÈ÷ ¿ëÇØÇÑ ÈÄ¿¡ »ç¿ëÇÑ´Ù.
  • Stop SolutionÀº ¾à»ê¼º ¼ººÐÀÌ Æ÷ÇԵǾî ÀÖÁö¸¸, ´«À̳ª ÇǺΠÁ¢ÃË¿¡ ÁÖÀÇÇÑ´Ù.

Keyword : Ȳ»ê,¹ÝÀÀÁ¤Áö¾×,wash buffer

EIA Kit
>
¼¼Æ÷ Á¢ÇÕ ÀÎÀÚ Fibronectin ƯÁ¤¿¡
Fibronectin EIA Kit
>
Human Albumin ¸ð´ÏÅ͸µ
Human Albumin EIA Kit
>
Human IgG ƯÀÌÀû Á¤·® Kit
Human IgG EIA Kit
>
ºñÆ®·Î³Øƾ ÃøÁ¤¿¡
Human Vitronectin EIA Kit
>
¿À½ºÅ׿À³Øƾ ÃøÁ¤¿¡
Human/Pig Osteonectin EIA Kit
>
Mouse Albumin ¸ð´ÏÅ͸µ
Mouse Albumin EIA Kit
>
°ñÇü¼º ¸¶Ä¿ ÃøÁ¤À» À§ÇÑ
Mouse Gla-Osteocalcin High Sensitive EIA Kit
>
°ñÇü¼º Marker ¡¤ °ñÈí¼ö Marker ÃøÁ¤
Osteocalcin EIA Kit
>
°ñÇü¼º ¸¶Ä¿, °ñÈí¼ö ¸¶Ä¿ ÃøÁ¤À» À§ÇÑ
Pig Osteocalcin EIA Kit
>
¼¼Æ÷ 1 Çü Collagen »ý»ê °ËÃâ
Procollagen Type I C-peptide (PIP) EIA Kit
>
°ñÇü¼º Marker, °ñÈí¼ö Marker ÃøÁ¤
Rat Gla-Osteocalcin High Sensitive EIA Kit
>
Stress Marker È¿¼Ò ÃøÁ¤
Rat Heme Oxygenase-1 EIA Kit