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Ç×ü ¡¤ ELISA ¡¤ Signal
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EIA Kit
> Wash and Stop Solution for ELISA without Sulfuric Acid
ELISA¿ë buffer ¼¼Æ®
Wash and Stop Solution for ELISA without Sulfuric Acid
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Takara
MK021
Wash and Stop Solution for ELISA without Sulfuric Acid
96 well plate ¡¿ 5 plates
59,200¿ø
74,000¿ø
09.02 ~ 10.31
Product index
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TaKaRa DAB Substrate
TaKaRa POD Conjugate½Ã..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã Ç×..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã..
EIA Kit
Acrolein-Lysine Adduct..
Fibronectin EIA Kit
Human Albumin EIA Kit
Human IgG EIA Kit
Human Vitronectin EIA ..
Human/Pig Osteonectin ..
Laminin(LN) EIA Kit
Mouse Albumin EIA Kit
Mouse Gla-Osteocalcin ..
Osteocalcin EIA Kit
Peptide Coating Kit
Pig Osteocalcin EIA Ki..
Procollagen Type I C-p..
Rat Gla-Osteocalcin Hi..
Rat Heme Oxygenase-1 E..
RetroNectin¢ç Á¤·® kit
Universal Tyrosine Kin..
Wash and Stop Solution..
Osteo¤ýAdipo assay
AdipoRed Assay Reagent
Osteoblast-Inducer Rea..
OsteoImage Mineralizat..
PDELight HTS cAMP Phos..
PPiLight Inorganic Pyr..
TRACP & ALP Assay Kit
TRACP & ALP double-sta..
¼¼Æ÷Áõ½Ä ¡¤ ¼¼Æ÷ÀåÇØ ÃøÁ¤
Premix WST-1 Cell Prol..
Luminescent Assay Acce..
Premix WST-1 Cell Prol..
ToxiLight BioAssay Kit
ViaLight ½Ã¸®Áî
È¿¼ÒÈ°¼º ÃøÁ¤ ¡¤ °ËÃâ¿ë Ki..
Heparan Degrading Enzy..
Apoptosis
ApoAlert Annexin V-FIT..
ApoAlert Caspase Assay..
ApoAlert pDsRed2-Bid V..
ApopLadder Ex¢â
ApoPrimer Set (Bcl-2 f..
In situ Apoptosis Dete..
Signal Transduction
Dominant-Negative Vect..
ExpressHyb Hybridizati..
Signal Àü´Þ °æ·Î Profi..
Ç×ü
Ç×ü ±³Â÷ ¹ÝÀÀ À϶÷Ç¥
Ç×ü ¿ëµµ À϶÷Ç¥
Anti-Albumin
Anti-Bovine Osteocalci..
Anti-CD3 mAb GMP grade
Anti-Chicken N-cadheri..
Anti-Heme Oxygenase-1
Anti-Human E-cadherin
Anti-Human Fibronectin
Anti-Human Influenza V..
Anti-Human Laminin
Anti-Human N-cadherin
Anti-Human Osteocalcin
Anti-Human P-cadherin
Anti-Human Platelet GM..
Anti-Human Procollagen..
Anti-Human Vitronectin
Anti-Human von Willebr..
Anti-Insulin C
Anti-Mouse E-cadherin
Anti-Mouse Osteocalcin
Anti-Mouse P-cadherin
Anti-Osteonectin/SPARC
Anti-ProS2
Anti-Rat Collagen type..
Anti-Rat Osteocalcin
Monoclonal 2nd antibod..
Polyclonal Anti-Dentin..
Polyclonal Anti-Glucag..
Polyclonal Anti-Human ..
Polyclonal Anti-Mouse ..
Polyclonal Anti-N-cadh..
Polyclonal Anti-Rat Bo..
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Wash and Stop Solution for ELISA without Sulfuric Acid´Â ´ÙÄ«¶ó EIA kit¸¦ ÀÌ¿ëÇÒ ¶§ ÇÊ¿äÇÑ washing solution (10 ¡¿ PBS, Tween20)°ú peroxidase substrate ¹ß»öÀ» ¸ØÃߴµ¥ »ç¿ëÇÏ´Â ¹ÝÀÀÁ¤Áö¾× (stop solution)À¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
º» Á¦Ç°¿¡ Æ÷ÇÔµÈ ¹ÝÀÀÁ¤Áö¾×Àº Ȳ»ê (Sulfuric acid)À» Æ÷ÇÔÇÏ°í ÀÖÁö ¾ÊÁö¸¸, ±âÁ¸ÀÇ ¹ÝÀÀÁ¤Áö¾× (1N H
2
SO
4
)°ú °°Àº ¹æ¹ý, ¿ë·®À¸·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À¸·Î Á¶Á¦ÇÑ Washing solution ¹× ¹ÝÀÀÁ¤Áö¾×Àº °¢ EIA kit¿¡ ¾È³»µÈ ¹æ¹ý°ú ¿ë·®¿¡ µû¶ó »ç¿ëÇÑ´Ù.
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1.
10 ¡¿ PBS
50 §¢ ¡¿ 5
2.
Tween20
3 §¢
3.
Stop Solution
60 §¢
º¸Á¸ ½Ç¿Â
»ç¿ë¹æ¹ý
Wash buffer
PBS wash buffer
: 10 ¡¿ PBS 1 bottle (50 §¢)À» ÃÖÁ¾ volume 500 §¢ÀÌ µÇµµ·Ï Áõ·ù¼ö·Î Èñ¼®ÇÑ´Ù.
ÃæºÐÈ÷ ¼¯Àº ÈÄ wash buffer·Î »ç¿ëÇÑ´Ù. (»ç¿ë½Ã Á¶Á¦ÇÒ °Í)
PBST (PBS + 0.1% Tween20) wash buffer
: 10 ¡¿ PBS 1 bottle (50 §¢)À» 450 §¢ÀÇ Áõ·ù¼ö¿¡ ¼¯¾î 1 ¡¿ PBS·Î Èñ¼®ÇÑ´Ù (ÃÖÁ¾ volume 500 §¢).
Tween20 500 §¡¸¦ ÷°¡ÇÑ ÈÄ, ÃæºÐÈ÷ ¼¯¾î¼ »ç¿ëÇÑ´Ù(»ç¿ë½Ã Á¶Á¦ÇÒ °Í).
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EIA kitÀÇ ÇÁ·ÎÅäÄÝ¿¡¼ ±ÇÀåÇÏ´Â wash buffer¿¡ µû¶ó PBS ¶Ç´Â PBST¸¦ ¼±ÅÃ
ÇÏ¿© »ç¿ëÇÑ´Ù (EIA kit¿¡ µû¶ó »ç¿ëÇÏ´Â wash buffer°¡ ´Ù¸¦ ¼ö ÀÖÀ½).
Stop Solution
Stop SolutionÀ» ±×´ë·Î »ç¿ëÇÑ´Ù.
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Stop SolutionÀº Á¡µµ°¡ ³ôÀº ¿ë¾×À̹ǷΠ÷°¡ ÈÄ plate mixer·Î ÃæºÐÈ÷ È¥ÇÕÇÑ´Ù.
»ç¿ë»óÀÇÁÖÀÇ
10 ¡¿ PBS´Â º¸°ü¿Âµµ°¡ ³·À» °æ¿ì ÇϾá ħÀü¹°ÀÌ »ý±æ ¼ö ÀÖ´Ù. ÀÌ °æ¿ì µû¶æÇÏ°Ô ÇÏ¿© ¿ÏÀüÈ÷ ¿ëÇØÇÑ ÈÄ¿¡ »ç¿ëÇÑ´Ù.
Stop SolutionÀº ¾à»ê¼º ¼ººÐÀÌ Æ÷ÇԵǾî ÀÖÁö¸¸, ´«À̳ª ÇǺΠÁ¢ÃË¿¡ ÁÖÀÇÇÑ´Ù.
Keyword : Ȳ»ê,¹ÝÀÀÁ¤Áö¾×,wash buffer
EIA Kit
Acrolein-Lysine Adduct Competitive EIA Kit
¼¼Æ÷ Á¢ÇÕ ÀÎÀÚ Fibronectin ƯÁ¤¿¡
Fibronectin EIA Kit
Human Albumin ¸ð´ÏÅ͸µ
Human Albumin EIA Kit
Human IgG ƯÀÌÀû Á¤·® Kit
Human IgG EIA Kit
ºñÆ®·Î³Øƾ ÃøÁ¤¿¡
Human Vitronectin EIA Kit
¿À½ºÅ׿À³Øƾ ÃøÁ¤¿¡
Human/Pig Osteonectin EIA Kit
Laminin(LN) EIA Kit
Mouse Albumin ¸ð´ÏÅ͸µ
Mouse Albumin EIA Kit
°ñÇü¼º ¸¶Ä¿ ÃøÁ¤À» À§ÇÑ
Mouse Gla-Osteocalcin High Sensitive EIA Kit
°ñÇü¼º Marker ¡¤ °ñÈí¼ö Marker ÃøÁ¤
Osteocalcin EIA Kit
Peptide Coating Kit
°ñÇü¼º ¸¶Ä¿, °ñÈí¼ö ¸¶Ä¿ ÃøÁ¤À» À§ÇÑ
Pig Osteocalcin EIA Kit
¼¼Æ÷ 1 Çü Collagen »ý»ê °ËÃâ
Procollagen Type I C-peptide (PIP) EIA Kit
°ñÇü¼º Marker, °ñÈí¼ö Marker ÃøÁ¤
Rat Gla-Osteocalcin High Sensitive EIA Kit
Stress Marker È¿¼Ò ÃøÁ¤
Rat Heme Oxygenase-1 EIA Kit
RetroNectin¢ç Á¤·® kit
Universal Tyrosine Kinase Assay Kit
ELISA¿ë buffer ¼¼Æ®
Wash and Stop Solution for ELISA without Sulfuric Acid