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TRACP & ALP Assay Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
MK301
TRACP & ALP Assay Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ȸ
502,400¿ø 
628,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
MK301_e.v1608Da.pdf

Á¦Ç° ¼³¸í
º» Á¦Ç°Àº ±âÁú pNPP (p-nitro-phenyl phosphate)¸¦ ÀÌ¿ëÇÏ°í, »ê¼º phosphotase (ACP) ¹× ¾ËÄ«¸®¼º phosphotase (ALP)ÀÇ È°¼ºÀ» °¢°¢ °ËÃâÇÏ´Â Å°Æ®ÀÌ´Ù. »ê¼º phosphotase °ËÃâ°è¿¡ ÁÖ¼®»êÀ» ÷°¡ÇÏ´Â °ÍÀ¸·Î ÆÄ°ñ¼¼Æ÷ÀÇ È¿¼Ò marker Áß ÁÖ¼®»ê ³»¼º »ê¼º phosphotase (TRACP)ÀÇ È°¼ºµµ¸¦ °ËÃâÇÒ ¼ö ÀÖ´Ù. °ñ¾Æ¼¼Æ÷ÀÇ È¿¼Ò marker Áß ¾ËÄ«¸®¼º phosphotase¿Í ÇÔ²² »À´ë»ç¿¡ °ü·ÃµÇ¾î 2 °³ÀÇ È¿¼Ò¸¦ µ¿½Ã¿¡ °ËÃâÇÒ ¼ö ÀÖ´Ù.
³»¿ë
1. pNPP (p-nitro-phenyl phosphate) substrate 24 mg ¡¿ 5
2. ¼¼Æ÷ ÃßÃâ¿ë ¿ë¾× 11 §¢ ¡¿ 2
3. ÁÖ¼®»ê ¿ë¾× 4 §¢
4. »ê¼º phosphatase¿ë buffer 30 §¢
5. ¾ËÄ«¸®¼º phosphatase¿ë buffer 30 §¢
6. 96 well plate 1 ¸Å
º¸Á¸ 4¡É
½ÇÇè¹ý
¼¼Æ÷ÀÇ ¼¼Á¤
    ¡é
¼¼Æ÷ÀÇ °¡¿ëÈ­
    ¡é
»ç¿ë bufferÀÇ ¼±ÅÃ
    ¡é
È¿¼Ò ¹ÝÀÀ 37¡É, 15-60ºÐ
    ¡é
Endpoint assay : ¹ÝÀÀÁ¤Áö(color formation)
Kinetic assay : ALPÀÇ °æ¿ì¸¸ °¡´É
    ¡é
405 nmÀÇ Èí±¤µµ ÃøÁ¤

Keyword : TRAP, Bone metabolism, Osteoblast, Osteoclast

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