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Tartrate-resistant acid phosphatase ¹× Alkaline Phosphatase 2 Áß ¿°»ö Kit

TRACP & ALP double-stain Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
MK300
TRACP & ALP double-stain Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
120 wells
(5 x 24 well plates)
400,800¿ø 
501,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
MK300_e.v2001Da.pdf

[±¸¼ºÇ° ¼ººÐ º¯°æ ¾È³»]
È­Çй°Áú°ü¸®±ÔÁ¦ °­È­·Î ÀÎÇÏ¿© Lot¹øÈ£ AJG0826A Á¦Ç°ºÎÅÍ(2020³â 2¿ù ÀÌÈÄ ÃâÇÏ¿¹Á¤)
±¸¼ºÇ° Áß ÇϳªÀÎ Fixation solution¿¡¼­ acetone°ú ethanolÀÌ Á¦¿ÜµÉ ¿¹Á¤ÀÌ¿À´Ï,
Acetone°ú ethanolÀº º°µµ·Î ÷°¡ÇÏ¿© »ç¿ë ¹Ù¶ø´Ï´Ù.

  • TRACP (TRAP)¿Í ALP¸¦ °¢°¢ ¶Ç´Â µ¿½Ã¿¡ ¿°»ö °¡´É
  • Premix ÇüÅÂÀÇ ½Ã¾àÀ¸·Î °£ÆíÇÑ »ç¿ë
  • Fixation solution°ú Nuclear stain ½Ã¾à ±âº» Æ÷ÇÔ
  • ¼¼Æ÷, Frozen section¿¡ ¿°»ö °¡´É [Paraffin section Àû¿ëºÒ°¡]
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Bone-metabolism (°ñ´ë»ç)¿¡ °ü·ÃµÈ ¼¼Æ÷¸¦ ¿°»öÇÏ´Â Á¦Ç°ÀÌ´Ù. Bone metabolismÀº Á¶°ñ¼¼Æ÷¿¡ ÀÇÇÑ °ñÇü¼º°ú ÆÄ°ñ¼¼Æ÷¿¡ ÀÇÇÑ °ñÈí¼öÀÇ »óÈ£ ÀÛ¿ëÀ¸·Î ÁøÇàµÈ´Ù. 2°³ÀÇ enzyme marker¸¦ µ¿½Ã¿¡ °ËÃâÇÏ¿©, °ñ °ü·Ã ¼¼Æ÷ÀÇ ºÐÈ­³ª °ñÁ¶Á÷ÀÇ ºÐÆ÷¸¦ °üÂû ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº Á¶°ñ¼¼Æ÷ (Osteoblasts)ÀÇ enzyme markerÀÎ Alkaline phosphatase (ALP)¿Í ÆÄ°ñ¼¼Æ÷ (Osteoclasts)ÀÇ enzyme markerÀÎ Tartrate-resistant acid phosphatase (TRACP, TRAP)¸¦ ¿°»öÇϱâ À§ÇÑ chromogenic substrates ¹× ÇÙ ¿°»ö ½Ã¾àÀ» Æ÷ÇÔÇÏ°í ÀÖ´Ù. TRACP & ALP double-stain Kit´Â 24 well plate ¶Ç´Â slide glass¿¡ ¹è¾çµÈ ¼¼Æ÷ÀÇ Acid ¹× Alkaline phosphatase È°¼ºÀ» °¢°¢ ¶Ç´Â µ¿½Ã¿¡ ¿°»öÇÏ¿© ºñ±³ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, ½Ã¾àÀº premix ÇüÅ·ΠÁ¦°øµÇ¾î °£ÆíÇÏ°Ô »ç¿ë °¡´ÉÇÏ´Ù.
°á°ú

±×¸² 1. 16ÁÖ JWÅä³¢ (¼öÄÆ)·ÎºÎÅÍ Ã¤ÃëÇÑ °ñ¼ö ¼¼Æ÷¿¡ M-CSF ¹× È°¼ºÇü ºñŸ¹Î D3 ó¸®ÇÏ¿© 6ÀÏ°£ ¹è¾ç ÈÄ TRACPÀÇ ¿°»öÀ» ½Ç½ÃÇÏ¿´´Ù.


±×¸² 2. »ýÈÄ 24ÀÏÀÇ SD rat (¾ÏÄÆ)À¸·ÎºÎÅÍ Ã¤ÃëÇÑ °ñ¼ö¼¼Æ÷¿¡ M-CSF¿Í È°¼ºÇü ºñŸ¹Î D3¸¦ ÷°¡ÇÏ¿© 10ÀÏ°£ ¹è¾ç ÈÄ ALP ¿°»öÀ» ½Ç½ÃÇÏ¿´´Ù.


±×¸² 3. Human Bone Marrow Mononuclear cells¿¡ ¿©·¯ °¡Áö ÷°¡ÀÎÀÚ¸¦ ÀÎÀÚ¸¦ ÷°¡ÇÏ¿© ¹è¾ç ÈÄ, ºÐÈ­°¡ µÇ¾úÀ» ¶§ (9ÀÏ) TRACP ¹× ALPÀÇ ¿°»öÀ» °¢°¢ ½Ç½ÃÇÏ¿´´Ù.
¿°»ö¿ø¸®
1) Acid phosphatase ¿°»öÀÇ ¿ø¸®
Naphthol-AS-BI-phosphate
    ¡éAcid phosphatase
HPO42-+ naphthol-AS
    ¡éFast Red Violet LB(Sodium azide)
Azo dye(ÀûÀÚ»ö)(pH5.2)

2) Alkaline phosphatase ¿°»öÀÇ ¿ø¸®
Bromo-Chloro-Indolyl phosphate
    ¡éAlkaline phosphatase
HPO42-+ Br-Cl-Indol
    ¡éNitro Blue Tetrazolium Chloride
Formazan dye(ûÀÚ»ö)(pH9.5)
³»¿ë

1. Fixation solution

30 §¢

2. Sodium tartrate

4 §¢

3. Substrate for ACP (premixed) (NABP/ FRVLB)

10 §¢¿ë¡¿3 ea

4. Substrate for ALP (premixed) (BCIP/ NBT)

10 §¢¿ë¡¿3 ea

5. Nuclear stain Methyl green

10 §¢

º¸Á¸ : -20¡É (»ç¿ë ÈÄ, °¢ ½Ã¾à¿¡ ÀûÀýÇÑ ¿Âµµ¿¡ º¸°ü ÇÒ °Í)

Keyword : TRAP, Bone metabolism, Osteoblast, Osteoclast

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