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PPiLight Inorganic Pyrophosphate Assay


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PPiLight Inorganic Pyrophosphate Assay
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가격문의   PPiLight_inorganic_pyrophosphate_assay.pdf

Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3) and ligases (EC 6).

The PPiLight™ Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin.

Figure 1: Bioluminescent reaction
  • Fast - Measure enzyme activity via pyrophosphate consumption in 1 hour
  • Simple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates
  • Wide Detection Range - Sensitive to 0.02 μM with a linear range to 10 μM
  • Versatile - Scalable to 96-, 384-, 1586-well formats
Measure activity of:
  • Phosphotransferases
  • Pyrophosphotases
  • Cyclases
  • Ligases
  • Hydrolases
  • DNA Polymerases

2°C - 8°C

Keyword :

OsteoㆍAdipo assay
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