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Ç×ü ¡¤ ELISA ¡¤ Signal
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Apoptosis
> ApoAlert Caspase Assay Kit
ApoAlert Caspase Assay Kit
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Clontech
630212
ApoAlert Caspase-9/6 Fluorescent Assay Kit
100 ȸ
1,586,000¿ø
Clontech
630215
ApoAlert Caspase-3 Fluorescent Assay Kit
100 ȸ
1,586,000¿ø
Clontech
630216
ApoAlert Caspase-3 Colorimetric Assay Kit
25 ȸ
603,000¿ø
Clontech
630217
ApoAlert Caspase-3 Colorimetric Assay Kit
100 ȸ
1,586,000¿ø
Product index
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TaKaRa DAB Substrate
TaKaRa POD Conjugate½Ã..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã Ç×..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã..
EIA Kit
Acrolein-Lysine Adduct..
Fibronectin EIA Kit
Human Albumin EIA Kit
Human IgG EIA Kit
Human Vitronectin EIA ..
Human/Pig Osteonectin ..
Laminin(LN) EIA Kit
Mouse Albumin EIA Kit
Mouse Gla-Osteocalcin ..
Osteocalcin EIA Kit
Peptide Coating Kit
Pig Osteocalcin EIA Ki..
Procollagen Type I C-p..
Rat Gla-Osteocalcin Hi..
Rat Heme Oxygenase-1 E..
RetroNectin¢ç Á¤·® kit
Universal Tyrosine Kin..
Wash and Stop Solution..
Osteo¤ýAdipo assay
AdipoRed Assay Reagent
Osteoblast-Inducer Rea..
OsteoImage Mineralizat..
PDELight HTS cAMP Phos..
PPiLight Inorganic Pyr..
TRACP & ALP Assay Kit
TRACP & ALP double-sta..
¼¼Æ÷Áõ½Ä ¡¤ ¼¼Æ÷ÀåÇØ ÃøÁ¤
Premix WST-1 Cell Prol..
Luminescent Assay Acce..
Premix WST-1 Cell Prol..
ToxiLight BioAssay Kit
ViaLight ½Ã¸®Áî
È¿¼ÒÈ°¼º ÃøÁ¤ ¡¤ °ËÃâ¿ë Ki..
Heparan Degrading Enzy..
Apoptosis
ApoAlert Annexin V-FIT..
ApoAlert Caspase Assay..
ApoAlert pDsRed2-Bid V..
ApopLadder Ex¢â
ApoPrimer Set (Bcl-2 f..
In situ Apoptosis Dete..
Signal Transduction
Dominant-Negative Vect..
ExpressHyb Hybridizati..
Signal Àü´Þ °æ·Î Profi..
Ç×ü
Ç×ü ±³Â÷ ¹ÝÀÀ À϶÷Ç¥
Ç×ü ¿ëµµ À϶÷Ç¥
Anti-Albumin
Anti-Bovine Osteocalci..
Anti-CD3 mAb GMP grade
Anti-Chicken N-cadheri..
Anti-Heme Oxygenase-1
Anti-Human E-cadherin
Anti-Human Fibronectin
Anti-Human Influenza V..
Anti-Human Laminin
Anti-Human N-cadherin
Anti-Human Osteocalcin
Anti-Human P-cadherin
Anti-Human Platelet GM..
Anti-Human Procollagen..
Anti-Human Vitronectin
Anti-Human von Willebr..
Anti-Insulin C
Anti-Mouse E-cadherin
Anti-Mouse Osteocalcin
Anti-Mouse P-cadherin
Anti-Osteonectin/SPARC
Anti-ProS2
Anti-Rat Collagen type..
Anti-Rat Osteocalcin
Monoclonal 2nd antibod..
Polyclonal Anti-Dentin..
Polyclonal Anti-Glucag..
Polyclonal Anti-Human ..
Polyclonal Anti-Mouse ..
Polyclonal Anti-N-cadh..
Polyclonal Anti-Rat Bo..
Caspase proteases play an essential role in apoptosis by degrading specific structural, regulatory, and DNA repair proteins within a cell (1, 2). Caspase-9 is activated very early in the apoptotic cascade by cytochrome c, which is released from the mitochondria in response to apoptotic stimuli (3). Activated caspase-9 then initiates the proteolytic activity of other downstream caspases, including caspase-3 and caspase-6. Caspase-8 also initiates the caspase pathway, directly or indirectly activating caspase-3 and other caspases (4, 5).
We offer caspase detection in two formats: assay kits and 96-well plates.
Caspase Assay Kits
The
ApoAlert Caspase Assay Kits
provide a simple and convenient way to detect caspase protease activity using fluorometric or colorimetric methods. The assay is simple to perform¡ªthe kits use crude cell lysates that can be prepared from as few as 1 x 106 adherent cells or cells in suspension. The assay kits detect caspase activation by assaying for the cleavage of a fluorescent substrate (Figure 1) or a colorimetric substrate.For high-throughput analysis, the assay can be performed in a microtiter plate. The entire protocol¡ªfrom collection of cells to fluorometric or colorimetric results¡ªtakes only 90 minutes.
The
Caspase-9/6 Fluorescent Assay
detects the shift in fluorescence of 7- amino-4-methoxy coumarin (AMC). Upon cleavage, AMC fluorescence can be measured using a 380-nm excitation filter and a 460-nm emission filter. Fluorescence detection is highly sensitive and can be used to measure even very small amounts of active caspase.
The
Caspase-3 and -8 Fluorescent Assays
detect the shift in fluorescence emission of 7-amino-4-trifluoromethyl coumarin (AFC). After the substrate is cleaved by the protease, AFC emits a yellow-green fluorescence at 505 nm if excited at 400 nm.
The
Caspase-3 and -8 Colorimetric Assays
measure the proteolytic cleavage of the chromophore p-nitroanilide (pNA) by caspase- 3 or -8. Liberated pNA can be monitored colorimetrically by absorbance at 405 nm.
Caspase Assay Plates
Oftentimes, depending upon the specific cell type as well as the apoptotic stimuli used, one or more caspases will become activated (Figure 2). The ApoAlert Caspase Profiling Assay Plate can be used to simultaneously analyze several different caspases in your sample, with up to 24 wells available for each caspase.
The
ApoAlert Caspase Profiling Plate
(Figure 3), measures the activation of four different caspases¡ªcaspase-2, -3, -8, and -9¡ªusing a fluorometric method in a convenient 96- well plate format. Caspase profiling plates make it easy to profile caspase activities in a variety of cell lines after treatment with different apoptotic stimuli.
The
ApoAlert Caspase-3 Assay Plate
provides a convenient way to monitor caspase-3 activity in up to 96 samples at once. No handling of caspase substrates is required, allowing for easy detection of caspase-3 activity in a variety of samples. Caspase-specific inhibitors are included for your control needs.
No Substrate Handling Required
Because the fluorogenic substrates specific for particular caspases are already immobilized in the wells of our 96-well plates, testing a variety of samples is easy, eliminating the need for additional handling of caspase substrates. Upon addition of a cell lysate containing activated caspase to the well, the immobilized fluorogenic substrate is cleaved by the corresponding activated caspase, releasing fluorescent product that can be easily detected by a standard fluorescence plate reader.
Figure 1. Detection of caspase activity during apoptosis.
The fluorescent and colorimetric assays are based on the same principle¡ªthey detect the fluorophore or chromophore that is cleaved from a substrate by the caspase protease.
Figure 2. Fluorometric detection of caspase activity during apoptosis.
After protease activation, the activated caspases recognize their respective substrates which are covalently linked to the fluorogenic dye, 7-amino-4- methyl coumarin (AMC). Upon cleavage by the respective caspase, the free dye can be detected using a plate reader with a 380 nm Excitation and 460 nm Emission filter.
Keyword :
Apoptosis
ApoAlert Annexin V-FITC Apoptosis Kit
ApoAlert Caspase Assay Kit
ApoAlert pDsRed2-Bid Vector
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ DNA °ËÃâ
ApopLadder Ex¢â
Bcl-2 À¯ÀüÀÚ RT-PCR¿¡
ApoPrimer Set (Bcl-2 family)
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
In situ Apoptosis Detection Kit