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30ºÐ ³» EV (Extracellular vesicle) °í¼øµµ ºÐ¸®

EV (Extracellular Vesicle) Isolation Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635741
Capturem¢â Extracellular Vesicle Isolation Kit (Mini)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ȸ
1,065,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x106497
Clontech
635748
Capturem¢â Extracellular Vesicle Isolation Kit (Maxi)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
8 Maxipreps
1,447,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x109323


* ¼¼Æ÷ »óÃþ¾×À¸·ÎºÎÅÍ ¸¹Àº ¾çÀÇ exosome ÃßÃâÀ» ¿øÇϽŴٸé? Capturem¢â Exosome Isolation Kit (Cell Culture) (Code 635723)
  • 30ºÐ ³» °í¼øµµ·Î ³óÃàµÈ EV ºÐ¸® - ÃÖ´ë 850 §¡ÀÇ Ç÷Àå, Ÿ¾×, ³úô¼ö¾× µî ü¾× »ùÇÃ, ¼¼Æ÷ ¹è¾ç »óÃþ¾×
  • ºÐ¸®µÈ EVÀÇ Exosome protein ƯÀÌÀû ¸¶Ä¿ ¹ßÇö È®ÀÎ - non-exosomal ´Ü¹éÁúÀÇ È¥ÀÔ ³·À½
  • ¸¹Àº ¾çÀÇ EV ºÐ¸® - exosomal RNA¸¦ ÀÌ¿ëÇÑ RT-qPCR, NGS ºÐ¼® Àû¿ë
  • ¼Õ»ó ¾øÀÌ EV ºÐ¸® - Åõ°ú ÀüÀÚ Çö¹Ì°æ (TEM)À» ÅëÇØ, ºÐ¸® ÈÄ EV ÇüÅ À¯Áö È®ÀÎ

 

Code

Ä÷³ ´ç Á¤Á¦ ¼öÀ²

Pre-ClearingÀ» À§ÇÑ º°µµ ±¸¸Å

Miniprep kit

635741

ÃÖ´ë 1010 yield

Amicon Ultra-0.5 Centrifugal Filter Unit, 100-KDa MWCO (Merck, Cat. No. UFC510024), (Thermo Fisher Scientific)

Maxiprep kit

635748

ÃÖ´ë 1011 yield

ºÒÇÊ¿ä (Á¦Ç° ³» Æ÷ÇÔ)

Á¦Ç°¼³¸í
Capturem¢â Extracellular Vesicle Isolation Kit´Â ¼Ò·®ÀÇ Ã¼¾× »ùÇ÷κÎÅÍ exosome°ú °°Àº EV (Extracellular vesicle)¸¦ °£ÆíÇÏ°í ½Å¼ÓÇÏ°Ô ºÐ¸®ÇÒ ¼ö ÀÖ´Ù. Ư¼öÇÏ°Ô Á¦ÀÛµÈ Capturem¢â spin columnÀ» »ç¿ëÇϸé Ç×ü¸¦ »ç¿ëÇÏÁö ¾Ê°í, lectin ±â¹ÝÀÇ °áÇÕ¹°ÁúÀ» ÀÌ¿ëÇØ Ç÷Àå, Ÿ¾×, ³úô¼ö¾×°ú °°Àº ü¾× ȤÀº ¼¼Æ÷ ¹è¾ç »óÃþ¾×À¸·ÎºÎÅÍ 30ºÐ À̳»¿¡ °í¼øµµ·Î ³óÃàµÈ EV¸¦ ºÐ¸®ÇÒ ¼ö ÀÖ´Ù. ȸ¼öµÈ EV´Â ´Ü¹éÁú ºÐ¼®, À¯ÀüÀÚ ºÐ¼®, Àü»çü ºÐ¼®¿¡ Àû¿ëÇϱ⿡ ÃæºÐÇÑ ¾çÀÌ´Ù.


±×¸² 1. Capturem¢â Extracellular Vesicle Isolation Kit¸¦ ÀÌ¿ëÇÑ ºü¸¥ EV Á¤Á¦ °úÁ¤.
Pre-clearing°úÁ¤¿¡¼­ »ùÇà ³» ¿À¿° ¹°ÁúÀ» Á¦°ÅÇϰí, EV°¡ ¾Æ´Ñ ´Ü¹éÁúÀ» Á¦°ÅÇϱâ À§ÇÏ¿© filtrationÀ» ÇÑ´Ù. ±× ÈÄ, »ùÇà ³»ÀÇ EVÀ» CapturemÀÇ membrane¿¡ °áÇÕ½ÃŲ ÈÄ, °¢ ´Ü°è¿¡ ÀûÇÕÇÑ ¹öÆÛ¸¦ ÀÌ¿ëÇØ Wash, Elute °úÁ¤À» ÁøÇàÇÑ´Ù. ¸ðµç °úÁ¤¿¡´Â 2ºÐ°£ÀÇ ÂªÀº ¿ø½ÉºÐ¸®°¡ ÇÊ¿äÇÏ´Ù. (*Filtration ½Ã°£Àº »ùÇÃÀÇ Á¾·ù¿Í ¾ç¿¡ µû¶ó ´Þ¶óÁú ¼ö ÀÖ´Ù.)


±×¸² 2. ºÐ¸®µÈ EV (Extracellular Vesicle)ÀÇ Å©±â º° ºÐÆ÷ ºñ±³.
Capturem¢â Extracellular Vesicle Isolation Kit¿Í ÃÊ¿ø½ÉºÐ¸®±â¸¦ ÀÌ¿ëÇØ, Ç÷Àå »ùÇà (N=3, 500 §¡)¿¡¼­ EV¸¦ ºÐ¸®ÇÏ¿© ³ª³ë ÀÔÀÚ ÃßÀû ºÐ¼® (NTA)À» ½Ç½ÃÇÏ¿´´Ù. EV Å©±âÀÇ Æò±ÕÀ» °ËÁ¤¼±, Ç¥ÁØ ¿ÀÂ÷¸¦ »¡°£¼±À¸·Î Ç¥½ÃÇÏ¿´´Ù. ÃÊ¿ø½ÉºÐ¸®±â¸¦ ÀÌ¿ëÇÏ¿© ºÐ¸®µÈ EV (Extracellular Vesicle) »ùÇÿ¡ ºñÇØ Capturem¢âÀ» »ç¿ëÇßÀ» ¶§, ÀϹÝÀûÀÎ EVÀÇ Å©±â ±âÁØÀ¸·Î »çÀÌÁî ºÐÆ÷ ¹üÀ§°¡ Á¼¾Ò´Ù. ÀÌ´Â Capturem¢âÀ» »ç¿ëÇßÀ» ¶§ º¸´Ù °í¼øµµÀÇ EV°¡ ºÐ¸®µÇ¾úÀ½À» ¾Ë ¼ö ÀÖ¾ú´Ù.


±×¸² 3. ´Ù¾çÇÑ »ùÇÿ¡¼­ÀÇ EV (Extracellular Vesicle) ºÐ¸® ¿¹.
¸ðÀ¯, Ÿ¾×, ³úô¼ö¾× (CSF), Ç÷û, ¼Òº¯, ¼¼Æ÷ ¹è¾ç »óÃþ¾×¿¡¼­ Capturem¢â Extracellular Vesicle Isolation Kit¸¦ ÀÌ¿ëÇØ EV¸¦ ºÐ¸®ÇÑ ÈÄ, ³ª³ë ÀÔÀÚ ÃßÀû ºÐ¼® (NTA)À» ½Ç½ÃÇÏ¿´´Ù. EVÀÇ Æò±Õ Å©±â¸¦ °ËÁ¤¼±, Ç¥ÁØ ¿ÀÂ÷¸¦ »¡°£¼±À¸·Î Ç¥½ÃÇÏ¿´´Ù. ¸ðµç »ùÇÿ¡¼­ EV°¡ ºÐ¸®µÇ¾úÀ¸¸ç, Capturem¢âÀÌ ´Ù¾çÇÑ »ùÇÃÀÇ EV ºÐ¸®¿¡ ÀÌ¿ëµÉ ¼ö ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.


±×¸² 4. Exosome marker¿Í Non-exosome ´Ü¹éÁúÀÇ °ËÃâ
Capturem¢â Extracellular Vesicle Isolation Kit¿Í ÃÊ¿ø½ÉºÐ¸®±â¸¦ ÀÌ¿ëÇØ Ç÷Àå¿¡¼­ EV¸¦ ºÐ¸®ÇÑ ÈÄ, exosome markerÀÎ CD63, CD9, Alix¿Í non-exosome ´Ü¹éÁúÀÎ calnexin, albuminÀ» western blottingÀ¸·Î °ËÃâÇÏ¿´´Ù. Capturem¢âÀ» ÀÌ¿ëÇØ ºÐ¸®ÇÑ EV¿¡¼­´Â exosome markerÀÎ CD63, CD9, AlixÀÇ ¹ßÇöÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú°í, non-exosome ´Ü¹éÁúÀÎ calnexin, albuminÀÇ ¹ßÇöÀÌ »ó´ëÀûÀ¸·Î ³·¾Ò´Ù.


±×¸² 5. RNA ¼öÀ²ÀÇ ºñ±³
Ç÷ûÀ» ÀÌ¿ëÇÏ¿© µÎ °¡Áö ¹æ¹ýÀ¸·Î EV¸¦ ÃßÃâÇÏ¿´´Ù (Capturem¢â 50 §¡, ÃÊ¿ø½ÉºÐ¸®±â 300 §¡). ºÐ¸®µÈ EV¿¡¼­ NucleoSpin miRNA Plasma (MN)À» ÀÌ¿ëÇØ miRNA¸¦ Á¤Á¦ÇÑ ÈÄ, Bioanalyzer¿¡¼­ RNA ¼öÀ²À» È®ÀÎÇß´Ù (Internal standard = 25 nt peak). µÎ ¹æ¹ýÀ¸·Î °¢°¢ ºÐ¸®ÇÑ EV¿¡¼­ ÃßÃâÇÑ RNA ¾çÀº Â÷À̰¡ ¾ø¾ú°í, µ¿ÀÏÇÑ ³óµµ°¡ È®ÀεǾú´Ù.
Àû¿ë
  • Next-generation sequencing (NGS)
  • Real Time PCR
  • Proteomics analysis
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
º¸Á¸ ½Ç¿Â

Keyword : Capturem,Exosome,¿¢¼ÒÁ»,Extracellular vesicle,EV,¼¼Æ÷ ¿Ü ¼ÒÆ÷,Exosome analysis,Protein analysis,´Ü¹éÁú ºÐ¼®,protein idenfication,´Ü¹éÁúµ¿Á¤,Protein isolation,´Ü¹éÁú ºÐ¸®

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