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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > In-Fusion Cloning > [Plus] In-Fusion¢ç Cloning

[Plus] In-Fusion¢ç Cloning

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Clontech
638909
In-Fusion¢ç HD Cloning Plus
LMOÁ¦Ç°  ¶óÀ̼±½º 
10 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32737, x32740, x33613
Clontech
638910
In-Fusion¢ç HD Cloning Plus
LMOÁ¦Ç°  ¶óÀ̼±½º 
50 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638911
In-Fusion¢ç HD Cloning Plus
LMOÁ¦Ç°  ¶óÀ̼±½º 
100 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638920
In-Fusion¢ç HD Cloning Plus
LMOÁ¦Ç°  ¶óÀ̼±½º 
96 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638916
In-Fusion¢ç HD Cloning Plus CE
LMOÁ¦Ç°  ¶óÀ̼±½º 
10 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32737, x32740, x33613
Clontech
638917
In-Fusion¢ç HD Cloning Plus CE
LMOÁ¦Ç°  ¶óÀ̼±½º 
50 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638918
In-Fusion¢ç HD Cloning Plus CE
LMOÁ¦Ç°  ¶óÀ̼±½º 
100 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638919
In-Fusion¢ç HD Cloning Plus CE
LMOÁ¦Ç°  ¶óÀ̼±½º 
96 ȸ
Á¾¸Å
Á¾¸ÅÀÏ :2023.02.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â


¡Ø º» Á¦Ç°Àº Á¾¸ÅµÇ¾ú½À´Ï´Ù. ¼º´ÉÀÌ Çâ»óµÈ In-Fusion® Snap Assembly ½Ã½ºÅÛÀ¸·Î ´ëüµÇ¾ú½À´Ï´Ù.
¡Ø In-Fusion Cloning »ý»ý Èı⺸±â

Product Name

Cat. No.

Size

Purification Kit Included

Stellar Competent Cells Included

CloneAmp HiFi PCR Premix Included

In-Fusion HD Cloning Plus

638909

10 rxns

Spin Columns

Yes

Yes

638910

50 rxns

Spin Columns

Yes

Yes

638911

100 rxns

Spin Columns

Yes

Yes

638920

96 rxns

Spin Columns

Yes

Yes

In-Fusion HD Cloning Plus CE

638916

10 rxns

Cloning Enhancer

Yes

Yes

638917

50 rxns

Cloning Enhancer

Yes

Yes

638918

100 rxns

Cloning Enhancer

Yes

Yes

638919

96rxns

Cloning Enhancer

Yes

Yes


Cloning Kit-PCR Based Seamless DNA Cloning with In-Fusion HD Cloning Plus
In-Fusion CloningÀº ¾î¶² PCR ´ÜÆíÀ̳ª º¹¼öÀÇ DNA ´ÜÆíµµ ¼±ÇüÈ­µÈ vector(linearized vector)¿¡ 15ºÐ(In-Fusion HD EcoDry´Â 30ºÐ)ÀÇ ¹ÝÀÀÀ¸·Î directional cloningÀÌ °¡´ÉÇÏ´Ù (Figure 1). ¶ÇÇÑ PCR ´ÜÆíÀº Á¦ÇÑÈ¿¼Ò󸮳ª phosphorylation°ú °°Àº Ãß°¡ÀûÀº ó¸® ´Ü°è°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù. In-Fusion È¿¼Ò´Â ¼±ÇüÈ­µÈ vector ¸»´Ü°ú PCR·Î ÁõÆøµÈ PCR ´ÜÆí(insert)ÀÇ ¸»´ÜÀÇ 15bp homologue sequence¸¦ ÀνÄÇÏ¿© À¶ÇÕ½ÃŲ´Ù. ÀÌ 15 bpÀÇ À¶ÇÕ¼­¿­Àº ¿øÇÏ´Â sequence¸¦ ÁõÆøÇϰíÀÚ ÇÏ´Â PCR primer¿¡ µðÀÚÀÎÇÏ¿© ²¸ ³ÖÀ¸¸é ½±°Ô ¸¸µé ¼ö ÀÖ´Ù.

ÀÌ ½ÇÇè¹æ¹ýÀ» »ç¿ëÇÏ¸é ¾Æ·¡¿Í °°Àº ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
  • Subcloning ¾øÀ̵µ º¹¼öÀÇ DNA ´ÜÆíÀ» ¿øÇÏ´Â vector¿¡ Cloning
  • ³»ºÎÀÇ Æ¯Á¤ ºÎºÐ¸¸ ±³Ã¼ÇÏ´Â Modular expression vector Á¦ÀÛ
  • ºÒÇÊ¿äÇÑ ¿°±â°¡ ¾ø´Â Fusion protein ±¸Ãà
  • DNA ´ÜÆíÀÇ Á¦°Å³ª ±³Ã¼
  • InsertÀÇ point mutationÀ̳ª Á¦ÇÑÈ¿¼Ò »çÀÌÆ® ÷°¡
  • À¯ÀüÀÚ³» Çü±¤ ´Ü¹éÁúÀÇ tag ±³Ã¼
  • cDNA·Î UTRs÷°¡



Figure 1. The In-Fusion cloning protocol.



Figure 2. The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).



Figure 3. The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

In-Fusion HD Cloning Plus Components
All In-Fusion HD Cloning Plus kits contain the In-Fusion HD enzyme premix and reagents for control experiments. In-Fusion HD Cloning Plus kits also include:
  • CloneAmp HiFi High Fidelity PCR Premix
    CloneAmp HiFi PCR Premix is a convenient 2X master mix that provides exceptionally accurate, efficient and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid set-up of PCR reactions, and facilitates successful cloning.
  • Stellar Competent Cells
    An E. coli HST08 strain is included that provides high transformation efficiency (greater than 5 x 108 cfu/¥ìg) and compliments the efficiency of the In-Fusion HD Cloning Plus kit.
  • Cloning Enhancer or NucleoSpin Gel and PCR Clean-Up
        o Cloning Enhancer (CE) is an easy-to-use enzyme premix that removes background plasmid DNA and PCR residue, eliminating
          the need for PCR insert purification prior to cloning. In-Fusion HD Cloning Plus CE kits are ideal for cloning when there is a single
          PCR product on the gel.
        o Alternatively, NucleoSpin Gel and PCR Clean-Up is suitable for gel extraction as well as PCR purification.

Keyword : infusion, In-fusion, ÀÎÇ»Á¯, ÀÎÇ»Àü, in fusion,Ŭ·Î´×, cloning, mutagenesis, mutation,soeasy,so easy