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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > hiPSC/hESC > Xeno-Free 3D Spheroid DEF-CS Culture Medium
Xeno FreeÀÇ human iPS/ES¼¼Æ÷ÀÇ 3D¹è¾ç¿ë ¹èÁö

Xeno-Free 3D Spheroid DEF-CS Culture Medium

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Cellartis
Y30047
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium, w/o antibiotics
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1 Kit
(Package)
°¡°Ý¹®ÀÇ   x32556


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º» Á¦Ç°Àº Àΰ£ À¯·¡ ´Ù´É¼º Áٱ⠼¼Æ÷(human ES / iPS ¼¼Æ÷)ÀÇ 3D spheroid ¹è¾ç¿ë ¹èÁö·Î µ¿¹°À̳ª Àΰ£ À¯·¡ ¼ººÐÀ» Æ÷ÇÔÇÏÁö ¾Ê´Â chemically defined mediaÀÌ´Ù. ¹è¾ç¿ë±â ¸éÀû¿¡ ÀÇÁ¸ÇÏÁö ¾Ê°í, ¹è¾ç ½ºÄÉÀÏ ¾÷¿¡ À¯¿ëÇÏ´Ù. º» Á¦Ç°¿¡¼­ ¹è¾çÇÑ Àΰ£ À¯·¡ ´Ù´É¼º Áٱ⠼¼Æ÷´Â ºÐÈ­ À¯µµ ´É·Â ¹× ÇÙÇüÀ» À¯ÁöÇÏ°í ÀÖ´Ù.

¡Ø Basal Medium¿¡´Â GMP grade Á¦Ç°(Code Y30071)µµ ÀÖ´Ù.
º» Á¦Ç° ÀÌ¿ë ½Ã Cellartis¢ç DEF-CS¢â 500 Xeno-Free 3D Spheroid Additives (Code Y30048)¿Í Á¶ÇÕÇؼ­ »ç¿ëÇϽʽÿÀ.
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Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o antibiotics (Code Y30047)

Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics

500 ml

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid additives

1 Set; ¹èÁö ÷°¡Á¦
(Code Y30048)

- DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000¡¿)

500 §¡

- DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000¡¿)

200 §¡

- DEF-CS Xeno-Free 3D Spheroid Additive 3 (500¡¿)

400 §¡

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Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o antibiotics : 4¡É
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid additives£º-15¡É ÀÌÇÏ

Application data

±×¸² 1. High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates grown under 4% O2 perfusion conditions were sequentially passaged three times by mechanical disruption (protocol adapted from Otsuji et al. 2014); cells were counted at passage on days 4 and 7, and at the final time point on day 11, to determine the expansion factor relative to the initial seeding number.


±×¸² 2. High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). hiPS cell aggregates were differentiated into definitive endoderm cells using a four-day protocol, then further differentiated into either lung progenitors (left), hepatospheres (middle), or beta cells (right) via respective 11-15 day differentiation protocols. qPCR data (not shown) indicated increases in FOXA2 and NKX2.1 expression in lung progenitors; CYP3A4, AFP, and CYP1A in hepatospheres; and PDX1 and NKX6.1 in beta cells.


±×¸² 3. High-quality hiPS cell aggregates grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). Representative images of aggregates at days 1, 2, and 5 after seeding with 2.5 x 105 cells/ml. Viable cells were labeled with fluorescein diacetate (green) and dead cells with propidium iodide (red).


±×¸² 4. High-quality hiPS cell aggregates were grown with Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium in stirred tank bioreactors (in perfusion mode). After expansion of hiPS cells as aggregates, cells were harvested on day 4 and expression of OCT4, SSEA-4, TRA-1-60 (pluripotency markers) and SSEA-1 and SOX17 (differentiation markers) was analyzed by immunostaining.

Keyword : spheroid,iPS,ES,Áٱ⼼Æ÷¹è¾ç,3D,3Dculture,Áٱ⼼Æ÷¹èÁö,xeno-free

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