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event
  : [θ] Lentivirus Cost Effectiveϰ ϱ Lenti-X ø
ۼ : 2024.06.05
 ۼ : īڸ

lentivirus transduction ؼ upstream ܰ lentivirus ̿ܿ downstream ܰ Ȯϰ ȿ Ǿ մϴ. īڸƿ Lentivirus titration, concentration, purification, transduction (enhancer) پ Cost Effectiveϰ ִ ǰ Ǹϰ ֽϴ. ȿ Kit ѹ ذ ð ϼ. 

 


Titration

Titer ϸ ̷ ִ ȰϿ ð ֽϴ. ϱ ؼ titration Ȯ MOI ڸ Ű ߿մϴ.

 

Lenti-X

 qRT-PCR Titration Kit

(Code 631235)

Lenti-X p24 Rapid Titer Kit (Single Wash)

(Code 631476)

Lenti-X GoStix Plus

(Code 631280 )

Ÿ

p24

lentiviral RNA

P24

ҿð

4ð

1.5ð

10

qRT-PCR

ELISA

Lateral flow

copies/ (viral RNA)

ng/(p24)

ng/(p24)

Ȯ

 

Concentration

Lentivirus 󵵰 ̸ ؼ ϰ մϴ. ̶ ultracentrifuge ϴ ⸦ ʿ ϸ ð ɸ մϴ. Lenti-X Concentrator (Code 631231 ) ϸ Ϲ ɺи ̸ ϰ ذ ֽϴ ( ).

Feature

Lenti-X Concentrator

(Code 631231)

Ultracentrifugation

Easily scalable

Yes

No

Specialized Equipment

No

Yes

Time Required

~1 hr

4 hr to overnight

Ease-of-Use

++++

+

Yield

90%

90%

 

Purification

Lentivirus in vivo ϴ 쿡 purification ʼԴϴ. ̶ filter ϴ lentivirus ڸ ջŰ 찡 ֽϴ. Lenti-X Maxi Purification Kit (Code 631233) gravity column ϱ lentivirus ջ ּȭϸ鼭 ȸ lenvirus ֽϴ.


Coming Soon!!

ο transduction enhancer, Lenti-X Transduction Sponge

Microfluidics lentivirus transduction ϴ ǰ õ˴ϴ. Spinoculation̳, chemical enhancer (e.g. Polybrene) ʰ ּȭϸ鼭 ð ȿ ȿ transduction ִ ǰԴϴ. Transduction cell-line (, ) primary cell پ ִ ǰ̴ Ź帳ϴ.


Lenti-X Transduction Spongeܰ

г A: ǰ ܰ ( 24)

г B: Ȯ (X 150), 20~300 microfluidics

г C: ɼ 24 well plate ű

г D: ̷ mixture ÷ 1ð incubationϴ

г E: Transduction (16~24ð incubation) 15 Ʃ ű

 

ǰ õ ǻ  (02-2081-2510, support@takara.co.kr ٶϴ 


÷: Lentivirus Cost Effectiveϰ ϱ Lenti-X ø.pdf