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Lenti-X Tet-On 3G Inducible Expression System

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
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Clontech
631187
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System
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3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631363
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha Version)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
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3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631351
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (Bicistronic Version)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
each
(Package)
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631349
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (with mCherry)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
each
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631350
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (with ZsGreen1)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
each
(Package)
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631354
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, Bicistronic)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
each
(Package)
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631352
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, mCherry)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
each
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200
Clontech
631353
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, ZsGreen1)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
each
(Package)
3,551,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32766, x32786, x33200

Ư¡
  • ³·Àº ¹é±×¶ó¿îµå, ³ôÀº À¯µµ¹ßÇö È¿À²
  • Lenti-X¢â Packaging Single Shots (VSV-G) Æ÷ÇÔ: °íÈ¿À², ¾ÈÁ¤¼º ³ôÀº ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡ ¿ëÀÌ
  • TRE3G promote·Î Á¤¹ÐÇÏ°Ô À¯ÀüÀÚ¹ßÇö Á¶Àý
Applications
  • ÇüÁúÀüȯÀÌ ¾î·Á¿î cell-type¿¡¼­ tetracycline À¯µµ ¹ßÇö
Tet-On¢ç Inducible Expression - The 3rd Generation
Tet-On¢ç 3G Tetracycline Inducible Gene Expression SystemsÀº °¡Àå °­·ÂÇÏ°í Àû¿ë¼ºÀÌ ¶Ù¾î³­ 3¼¼´ë Æ÷À¯·ù À¯µµ ¹ßÇö ½Ã½ºÅÛÀ¸·Î Á¤¹ÐÇÏ°Ô À¯ÀüÀÚ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖµµ·Ï ±âÁ¸ Á¦Ç°ÀÇ ¼º´ÉÀ» °³¼±ÇÏ¿´´Ù. º» ½Ã½ºÅÛÀ» ÀÌ¿ëÇϸé Á» ´õ °¡¿ªÀûÀÌ°í Á¤·®ÀûÀ̸ç ÀçÇö¼º ÀÖ°Ô ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖ´Ù. Hermann Bujard¿Í Manfred Gossen, Wolfgang Hillen ¿¬±¸ ±×·ì(1,2)¿¡ ÀÇÇØ °³¹ßµÈ 3G ½Ã½ºÅÛÀº promoter º¯ÇüÀ¸·Î ¹é±×¶ó¿îµå¸¦ ÇöÀúÇÏ°Ô ÁÙÀÌ°í transactivator protein °³¼±À» ÅëÇØ Dox¿¡ ´ëÇÑ ¹Î°¨µµ¸¦ ÇÑÃþ ³ôÀÎ Çõ½ÅÀûÀÎ Tet ½Ã½ºÅÛÀÌ´Ù.
Tet-On¢ç 3G Inducible SystemÀÇ °³¿ä
Tet-On¢ç 3G transactivator proteinÀ» ¹ßÇöÇϸ鼭 TRE3G promoter (PTRE3G)·Î ¸ñÀû À¯ÀüÀÚ¸¦ Á¶ÀýÇÏ´Â Tet-On¢ç 3G ¼¼Æ÷´Â µ¶½Ã»çÀÌŬ¸°(Dox; tetracycline analog)ÀÌ Ã·°¡µÇ¾úÀ» ¶§ ¸ñÀû À¯ÀüÀÚ¸¦ °í¹ßÇö ½ÃŲ´Ù. Tet-On¢ç 3G proteinÀº Dox°¡ °áÇÕÇϸé ÇüÅÂÀûÀÎ º¯È­°¡ »ý°Ü PTRE3G ³»ÀÇ tet operator (tet O)¿¡ °áÇÕÇÏ°Ô µÈ´Ù (±×¸² 1). »õ·Î¿î ½Ã½ºÅÛÀº ±âÁ¸ÀÇ ³·Àº ¼öÁØÀÇ TetR-based system (Clontech¿¡¼­´Â ÆǸÅÇÏÁö ¾Ê´Â Á¦Ç°)°ú´Â ´Þ¸®, Tet-On¢ç ±â¼úÀ» Àû¿ëÇÏ¿© transcriptionÀ» ÀúÇØÇϱ⠺¸´Ù´Â ÃËÁø½ÃÅ°´Â °ÍÀ¸·Î ±Øµµ·Î ³·Àº ±âÀú¹ßÇö°ú ³ôÀº ¸ñÀû À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇÏ´Ù. »Ó¸¸ ¾Æ´Ï¶ó ÇÑÃþ ´õ »¡¶óÁø ¹ÝÀÀ½Ã°£ µîÀÇ ÀåÁ¡À» °®°í ÀÖÀ¸¸ç ƯÈ÷ in vivo ½ÇÇè¿¡ ÃÖÀûÀÌ´Ù.


±×¸² 1. The Tet-On¢ç 3G Systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G Promoter.
±âº»ÀûÀÎ Á¦Ç° ±¸¼ºÇ°
General System Components
  • 10ug     regulator vector
  • 10ug     response vector
  • 10ug     pLVX-TRE3G-Luc Control Vector
  • 16rxns   Lenti-X¢â Packaging Single shots (VSV-G)
  • 3rxns     Lenti-X¢â GoStix Plus

Keyword :