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Inducible System ¡¤ Yeast hybrid
>
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> Lenti-X Tet-On 3G Inducible Expression System
Lenti-X Tet-On 3G Inducible Expression System
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Clontech
631187
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System
each
(Package)
3,551,000¿ø
,
,
Clontech
631363
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha Version)
Each
(Package)
3,551,000¿ø
,
,
Clontech
631351
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (Bicistronic Version)
each
(Package)
3,551,000¿ø
,
,
Clontech
631349
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (with mCherry)
each
3,551,000¿ø
,
,
Clontech
631350
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (with ZsGreen1)
each
(Package)
3,551,000¿ø
,
,
Clontech
631354
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, Bicistronic)
each
(Package)
3,551,000¿ø
,
,
Clontech
631352
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, mCherry)
each
3,551,000¿ø
,
,
Clontech
631353
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, ZsGreen1)
each
(Package)
3,551,000¿ø
,
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
Ư¡
³·Àº ¹é±×¶ó¿îµå, ³ôÀº À¯µµ¹ßÇö È¿À²
Lenti-X¢â Packaging Single Shots (VSV-G)
Æ÷ÇÔ: °íÈ¿À², ¾ÈÁ¤¼º ³ôÀº ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡ ¿ëÀÌ
TRE3G promote·Î Á¤¹ÐÇÏ°Ô À¯ÀüÀÚ¹ßÇö Á¶Àý
Applications
ÇüÁúÀüȯÀÌ ¾î·Á¿î cell-type¿¡¼ tetracycline À¯µµ ¹ßÇö
Tet-On
¢ç
Inducible Expression - The 3rd Generation
Tet-On
¢ç
3G Tetracycline Inducible Gene Expression SystemsÀº °¡Àå °·ÂÇÏ°í Àû¿ë¼ºÀÌ ¶Ù¾î³ 3¼¼´ë Æ÷À¯·ù À¯µµ ¹ßÇö ½Ã½ºÅÛÀ¸·Î Á¤¹ÐÇÏ°Ô À¯ÀüÀÚ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖµµ·Ï ±âÁ¸ Á¦Ç°ÀÇ ¼º´ÉÀ» °³¼±ÇÏ¿´´Ù. º» ½Ã½ºÅÛÀ» ÀÌ¿ëÇϸé Á» ´õ °¡¿ªÀûÀÌ°í Á¤·®ÀûÀ̸ç ÀçÇö¼º ÀÖ°Ô ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖ´Ù. Hermann Bujard¿Í Manfred Gossen, Wolfgang Hillen ¿¬±¸ ±×·ì(1,2)¿¡ ÀÇÇØ °³¹ßµÈ 3G ½Ã½ºÅÛÀº promoter º¯ÇüÀ¸·Î ¹é±×¶ó¿îµå¸¦ ÇöÀúÇÏ°Ô ÁÙÀÌ°í transactivator protein °³¼±À» ÅëÇØ Dox¿¡ ´ëÇÑ ¹Î°¨µµ¸¦ ÇÑÃþ ³ôÀÎ Çõ½ÅÀûÀÎ Tet ½Ã½ºÅÛÀÌ´Ù.
Tet-On
¢ç
3G Inducible SystemÀÇ °³¿ä
Tet-On
¢ç
3G transactivator proteinÀ» ¹ßÇöÇÏ¸é¼ TRE3G promoter (PTRE3G)·Î ¸ñÀû À¯ÀüÀÚ¸¦ Á¶ÀýÇÏ´Â Tet-On
¢ç
3G ¼¼Æ÷´Â µ¶½Ã»çÀÌŬ¸°(Dox; tetracycline analog)ÀÌ Ã·°¡µÇ¾úÀ» ¶§ ¸ñÀû À¯ÀüÀÚ¸¦ °í¹ßÇö ½ÃŲ´Ù. Tet-On
¢ç
3G proteinÀº Dox°¡ °áÇÕÇϸé ÇüÅÂÀûÀÎ º¯È°¡ »ý°Ü PTRE3G ³»ÀÇ tet operator (tet O)¿¡ °áÇÕÇÏ°Ô µÈ´Ù (±×¸² 1). »õ·Î¿î ½Ã½ºÅÛÀº ±âÁ¸ÀÇ ³·Àº ¼öÁØÀÇ TetR-based system (Clontech¿¡¼´Â ÆǸÅÇÏÁö ¾Ê´Â Á¦Ç°)°ú´Â ´Þ¸®, Tet-On
¢ç
±â¼úÀ» Àû¿ëÇÏ¿© transcriptionÀ» ÀúÇØÇϱ⠺¸´Ù´Â ÃËÁø½ÃÅ°´Â °ÍÀ¸·Î ±Øµµ·Î ³·Àº ±âÀú¹ßÇö°ú ³ôÀº ¸ñÀû À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇÏ´Ù. »Ó¸¸ ¾Æ´Ï¶ó ÇÑÃþ ´õ »¡¶óÁø ¹ÝÀÀ½Ã°£ µîÀÇ ÀåÁ¡À» °®°í ÀÖÀ¸¸ç ƯÈ÷ in vivo ½ÇÇè¿¡ ÃÖÀûÀÌ´Ù.
±×¸² 1. The Tet-On
¢ç
3G Systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G Promoter.
±âº»ÀûÀÎ Á¦Ç° ±¸¼ºÇ°
General System Components
10ug regulator vector
10ug response vector
10ug pLVX-TRE3G-Luc Control Vector
16rxns Lenti-X¢â Packaging Single shots (VSV-G)
3rxns Lenti-X¢â GoStix Plus
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ