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Tet-On 3G 유도 발현 시스템

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Clontech
 631164
 Tet-On 3G Inducible Expression System (with ZsGreen1)
관련학술 구매하기 라이선스  		Each
 1,983,000원  제조사 페이지로 바로가기 x33302, x33594, x33597, x33151, x33200
Clontech
 631165
 Tet-On 3G Inducible Expression System (with mCherry)
관련학술 구매하기 라이선스  		Each
 1,983,000원  제조사 페이지로 바로가기 x33302, x33594, x99952, x33596, x99953, x33200, x33151
Clontech
 631167
 Tet-On 3G Inducible Expression System (EF1α Version)
관련학술 구매하기 라이선스  		Each
 1,983,000원  제조사 페이지로 바로가기 x33362, x33584, x33151, x33200
Clontech
 631168
 Tet-On 3G Inducible Expression System
관련학술 구매하기 라이선스  		Each
 1,983,000원  제조사 페이지로 바로가기 x33302, x33584, x33594, x33151, x33200
Clontech
 631187
 Lenti-X™ Tet-On® 3G Inducible Expression System
관련학술 구매하기 라이선스  		each
 3,415,000원  제조사 페이지로 바로가기 x32766, x32786, x33200
Clontech
 631188
 Retro-X Tet-On 3G Inducible Expression System
관련학술 구매하기 LMO제품  라이선스  		Each
 3,046,000원  제조사 페이지로 바로가기 x33346, x33548, x33557, x33558, x33014, x33200
Clontech
 631348
 Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)
관련학술 구매하기 라이선스  		each
 2,338,000원  제조사 페이지로 바로가기 x33362, x33594, x33597, x33151
Clontech
 631337
 Tet-On 3G Bidirectional Inducible Expression System
관련학술 구매하기 라이선스  		Each
 2,204,000원  제조사 페이지로 바로가기 x33302, x33586, x33588, x33151
Clontech
 631338
 Tet-On 3G Bidirectional Inducible Expression System (with mCherry)
관련학술 구매하기 라이선스  		Each
 2,338,000원  제조사 페이지로 바로가기 x33302, x33588, x33589, x33151
Clontech
 631339
 Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)
관련학술 구매하기 라이선스  		Each
 2,338,000원  제조사 페이지로 바로가기 x33302, x33588, x33590, x33151
Clontech
 631340
 Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version)
관련학술 구매하기 라이선스  		Each
 2,204,000원  제조사 페이지로 바로가기 x33362, x33586, x33588, x33151
Clontech
 631341
 Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)
관련학술 구매하기 라이선스  		Each
 2,338,000원  제조사 페이지로 바로가기 x33362, x33588, x33589, x33151
Clontech
 631342
 Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)
관련학술 구매하기 라이선스  		Each
 2,338,000원  제조사 페이지로 바로가기 x33362, x33588, x33590, x33151
Clontech
 631363
 Lenti-X™ Tet-On® 3G Inducible Expression System (EF1alpha Version)
관련학술 구매하기 라이선스  		Each
 3,415,000원  제조사 페이지로 바로가기 x32766, x32786, x33200

Tet-On Inducible Expression - The 3rd Generation
The Tet-On 3G Tetracycline Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The 3G system offers a significant improvement over Tet-On and Tet-On Advanced by further reducing basal expression and increasing sensitivity to doxycycline, a tetracycline analogue (Table I, Figures 1-3).
표 1
Tet-On 3G: Lowest Background Ever, Highest Sensitivity, Highest Fold Induction
Lowest Background
Mutations within the PTRE3G promoter have reduced background expression from this inducible promoter by 5-20 fold compared to our previous tightest promoter PTight (Figure 1).

Highest Sensitivity
Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox), so that at very low Dox concentrations (between 5-10 ng/ml), expression can be 100-150 fold higher when using a Tet-On 3G system, compared to Tet-On Advanced (Figure 3). An overnight exposure to as little as 10 ng/ml of Dox generates very high expression levels (Figure 4).

Highest Fold Induction
Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher (Figure 2).

We have observed unprecedented fold induction levels even in transient cotransfection experiments-up to a 27,000 fold difference between the induced and uninduced expression levels in MCF7 cells (not shown).

To learn about the mechanism of tetracycline-controlled inducible expression and the changes made to both the transactivator and TRE promoter creating the Tet-On 3G Inducible Expression System, visit our Tet-On 3G resource site or read the inducible expression technical note.
Figure 1. The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On Advanced Inducible Expression Systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G System produces far lower background expression in the absence of Dox (inset).

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