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Inducible System ¡¤ Yeast hybrid
>
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
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Clontech
631164
Tet-On 3G Inducible Expression System (with ZsGreen1)
Each
1,983,000¿ø
,
,
,
,
Clontech
631165
Tet-On 3G Inducible Expression System (with mCherry)
Each
1,983,000¿ø
,
,
,
,
,
,
Clontech
631167
Tet-On 3G Inducible Expression System (EF1¥á Version)
Each
1,983,000¿ø
,
,
,
Clontech
631168
Tet-On 3G Inducible Expression System
Each
1,983,000¿ø
,
,
,
,
Clontech
631187
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System
each
3,415,000¿ø
,
,
Clontech
631188
Retro-X Tet-On 3G Inducible Expression System
Each
3,046,000¿ø
,
,
,
,
,
Clontech
631348
Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)
each
2,338,000¿ø
,
,
,
Clontech
631337
Tet-On 3G Bidirectional Inducible Expression System
Each
2,204,000¿ø
,
,
,
Clontech
631338
Tet-On 3G Bidirectional Inducible Expression System (with mCherry)
Each
2,338,000¿ø
,
,
,
Clontech
631339
Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)
Each
2,338,000¿ø
,
,
,
Clontech
631340
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version)
Each
2,204,000¿ø
,
,
,
Clontech
631341
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)
Each
2,338,000¿ø
,
,
,
Clontech
631342
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)
Each
2,338,000¿ø
,
,
,
Clontech
631363
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha Version)
Each
3,415,000¿ø
,
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
AD Insert ÁõÆø¿ë Prime..
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
Tet-On Inducible Expression - The 3rd Generation
The Tet-On 3G Tetracycline Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The 3G system offers a significant improvement over Tet-On and Tet-On Advanced by further reducing basal expression and increasing sensitivity to doxycycline, a tetracycline analogue (Table I, Figures 1-3).
Tet-On 3G: Lowest Background Ever, Highest Sensitivity, Highest Fold Induction
Lowest Background
Mutations within the PTRE3G promoter have reduced background expression from this inducible promoter by 5-20 fold compared to our previous tightest promoter PTight (Figure 1).
Highest Sensitivity
Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox), so that at very low Dox concentrations (between 5-10 ng/ml), expression can be 100-150 fold higher when using a Tet-On 3G system, compared to Tet-On Advanced (Figure 3). An overnight exposure to as little as 10 ng/ml of Dox generates very high expression levels (Figure 4).
Highest Fold Induction
Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher (Figure 2).
We have observed unprecedented fold induction levels even in transient cotransfection experiments-up to a 27,000 fold difference between the induced and uninduced expression levels in MCF7 cells (not shown).
To learn about the mechanism of tetracycline-controlled inducible expression and the changes made to both the transactivator and TRE promoter creating the Tet-On 3G Inducible Expression System, visit our Tet-On 3G resource site or read the inducible expression technical note.
Figure 1. The PTRE3G promoter results in significantly reduced basal expression.
HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On Advanced Inducible Expression Systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G System produces far lower background expression in the absence of Dox (inset).
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ