¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
Inducible System ¡¤ Yeast hybrid
>
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> Tet-On 3G Cell Line
Tet-On 3G Cell Line
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
631183
HeLa Tet-On 3G Cell Line
Each
(Package)
2,134,000¿ø
,
Clontech
631182
HEK 293 Tet-On 3G Cell Line
Each
(Package)
2,134,000¿ø
,
Clontech
631195
CHO Tet-On 3G Cell Line
Each
(Package)
2,134,000¿ø
,
Clontech
631197
NIH/3T3 Tet-On 3G Cell Line
Each
(Package)
2,134,000¿ø
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
[ÁÖÀÇ]
µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
NOTE:
We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.
Tet-On Advanced and Tet-On inducible cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium (Figure 1; 1, 2). Our Tet-Off¢ç Advanced and Tet-Off inducible cell lines are also available, which are activated by removing Dox from the culture medium.
These rigorously tested clonal cell lines:
Stably express either the second-generation Tet-On Advanced transactivator protein or the original Tet-On transactivator protein
Allow you to induce expression of your gene-of-interest by adding doxycycline (Dox) to the culture of transfected cells
Can save you up to two months of time in developing a complete Tet-controlled system.
Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTight in pTRE-Tight), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.
Our PC12 Tet-On Cell Line responds normally to nerve growth factor (NGF). For more details, see our report Neural Differentiation in Tet-Inducible PC12 Cell Lines.
References
1. Urlinger, S., et al. (2000) Proc. Natl. Acad. Sci. USA 97(14):7963-7968.
2. Gossen, M., et al. (1995) Science 268:1766-1769.
Components
Tet-On or Tet-On Advanced Cell Line
U2-OS-Luc Tet-On Control Cell Line
Tet System Approved FBS (50 ml)
Storage Conditions
-196¡ÆC
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ