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Yeast Transformation System 2

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
630439
Yeastmaker¢â Yeast Transformation System 2
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
(Package)
652,000¿ø 
815,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33370, x33213
Clontech
630440
Yeastmaker¢â Carrier DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿5
573,600¿ø 
717,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
630440-PA06729[0].pdf

  • ³ôÀº transformation È¿À²
  • Plasmid ¹× library ±Ô¸ðÀÇ transformation¿¡ ÀûÇÕ
  • ÃÖÀûÈ­µÈ carrier DNA¿Í YPD Plus¸¦ ÇÔ²² »ç¿ëÇϸé transformantionÀÇ È¿À²À» ³ôÀÏ ¼ö ÀÖ´Ù.
Á¦Ç°¼³¸í
Yeastmaker¢â Yeast Transformation System 2´Â yeast competent cellÀ» ÁغñÇÏ°í transformation Çϱâ À§ÇÑ °íÈ¿À²ÀÇ polyethylene glycol (PEG)/LiAc ¹æ¹ýÀ» ±â¹ÝÀ¸·Î ÇÏ´Â Á¦Ç°ÀÌ´Ù. Á¦Ç° ³»¿¡ Æ÷ÇԵǾîÀÖ´Â YPD Plus Liquid Medium°ú º°µµ·Î ÆǸÅÇÏ°í ÀÖ´Â Yeast maker Carrier DNA (Cat. No. 630440)¿Í ÇÔ²² »ç¿ëÇϸé Á¶ÀÛ °úÁ¤ÀÌ °£´ÜÇÏ¿© ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â ´Ù¸¥ ¹æ¹ýº¸´Ù Á» ´õ È¿À²ÀûÀÌ°í È®½ÇÇÏ°Ô ÇüÁú Àüȯ½Ãų ¼ö ÀÖ´Ù.
ÀÌ Á¦Ç°Àº 0.1~1.0 ¥ìgÀÇ plasmid DNA¸¦ »ç¿ëÇÏ¿© 50 ȸ Á¤µµ ÇüÁú Àüȯ ÇÒ ¼ö ÀÖÀ¸¸ç, 1~10 ¥ìgÀÇ plasmid DNA¸¦ »ç¿ëÇÏ¿© 15 ȸ ÇüÁú Àüȯ ÇÒ ¼ö ÀÖ´Ù.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ

Box 1:
  • 2 x 1 ml Yeastmaker¢â Carrier DNA, (10 mg/ml)
  • 20 ¥ìl pGBT9 (0.1 ¥ìg/¥ìl; positive control plasmid)

Box 2:
  • 2 x 50 ml 50% PEG
  • 50 ml 1 M LiAc (10X)
  • 50 ml 10X TE Buffer
  • 50 ml YPD Plus Liquid Medium

Keyword :