´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.

Selection marker (Linear / Plasmid)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631625
Linear Hygromycin Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
2 ¥ìg
332,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631626
Linear Puromycin Marker
°ü·ÃÇмú ±¸¸ÅÇϱâ
2 ¥ìg
332,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631601
pPUR Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
781,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33527
Clontech
631750
pTK-Hyg Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¥ìg
806,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33580, x32774

  • Stable cell lineÀ» ¸¸µé±â À§ÇÏ¿© ¹ßÇö vector¿Í cotransfection
  • Linear Selection Marker´Â transfection½Ã ±âÁ¸º¸´Ù ÀûÀº ¾çÀÇ DNA »ç¿ë
  • Circular plasmid marker°¡ transfection¿¡ ÇÔ²² »ç¿ëµÇ¾úÀ» ¶§º¸´Ù ´õ ¸¹Àº positive colony ȹµæ
Linear Selection Marker´Â ¾ÈÁ¤ÀûÀÎ »ðÀÔ°ú ¹ßÇöÀÌ ÇÊ¿äÇÑ °æ¿ì ¹ßÇö vector¿Í ÇÔ²² cotransfectionÇÒ ¶§ ÀûÇÕÇÏ´Ù. À̵é marker´Â marker gene, SV40 promoter, SV40 polyadenylation signal·Î ±¸¼ºµÈ ª°í Á¤Á¦µÈ linear DNA´ÜÆíÀÌ´Ù.
Linear Selection Marker¸¦ ÇÔ²² cotransfection Çϸé selectable marker¸¦ ÇÔÀ¯ÇÑ single response vector ¶Ç´Â circular selectabe marker¿Í ÇÔ²² cotransfectionÇÏ´Â °Íº¸´Ù ´õ ¸¹Àº positive cloneµéÀ» ¾òÀ» ¼ö ÀÖ´Ù (Table I). ¶ÇÇÑ, ClontechÀº puromycin°ú hygromycin ´ëÇÑ standard plasmid ±â¹ÝÀÇ selectable marker¸¦ Á¦°øÇÏ°í ÀÖ´Ù.
Table I: Positive Clones Generated from Different Transfection Methods
Vector # Positive Clones % Positive Clones
Hygromycin
pTRE2hyg 8/27 30%
pTK-Hyg* 2/5 40%
Linear Hyg* 22/28 79%
Puromycin
pTRE2pur 4/11 36%
pPUR* 3/7 42%
Linear Pur* 6/8 75%
* For these cotransfections the ratio of selection marker to expression vector (pTRE-Tight-Luc) was 1:20.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Linear Selection Markers (April 2003) Clontechniques XVIII(2):11.

Keyword :