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Inducible System ¡¤ Yeast hybrid
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Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> Selection marker (Linear / Plasmid)
Selection marker (Linear / Plasmid)
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(ºÎ°¡¼¼º°µµ)
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»ç¿ëÀڸŴº¾ó
Clontech
631625
Linear Hygromycin Marker
2 ¥ìg
332,000¿ø
Clontech
631626
Linear Puromycin Marker
2 ¥ìg
332,000¿ø
Clontech
631601
pPUR Vector
25 ¥ìg
781,000¿ø
Clontech
631750
pTK-Hyg Vector
10 ¥ìg
806,000¿ø
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
Stable cell lineÀ» ¸¸µé±â À§ÇÏ¿© ¹ßÇö vector¿Í cotransfection
Linear Selection Marker´Â transfection½Ã ±âÁ¸º¸´Ù ÀûÀº ¾çÀÇ DNA »ç¿ë
Circular plasmid marker°¡ transfection¿¡ ÇÔ²² »ç¿ëµÇ¾úÀ» ¶§º¸´Ù ´õ ¸¹Àº positive colony ȹµæ
Linear Selection Marker´Â ¾ÈÁ¤ÀûÀÎ »ðÀÔ°ú ¹ßÇöÀÌ ÇÊ¿äÇÑ °æ¿ì ¹ßÇö vector¿Í ÇÔ²² cotransfectionÇÒ ¶§ ÀûÇÕÇÏ´Ù. À̵é marker´Â marker gene, SV40 promoter, SV40 polyadenylation signal·Î ±¸¼ºµÈ ª°í Á¤Á¦µÈ linear DNA´ÜÆíÀÌ´Ù.
Linear Selection Marker¸¦ ÇÔ²² cotransfection Çϸé selectable marker¸¦ ÇÔÀ¯ÇÑ single response vector ¶Ç´Â circular selectabe marker¿Í ÇÔ²² cotransfectionÇÏ´Â °Íº¸´Ù ´õ ¸¹Àº positive cloneµéÀ» ¾òÀ» ¼ö ÀÖ´Ù (Table I). ¶ÇÇÑ, ClontechÀº puromycin°ú hygromycin ´ëÇÑ standard plasmid ±â¹ÝÀÇ selectable marker¸¦ Á¦°øÇÏ°í ÀÖ´Ù.
Table I: Positive Clones Generated from Different Transfection Methods
Vector
# Positive Clones
% Positive Clones
Hygromycin
pTRE2hyg
8/27
30%
pTK-Hyg
*
2/5
40%
Linear Hyg
*
22/28
79%
Puromycin
pTRE2pur
4/11
36%
pPUR
*
3/7
42%
Linear Pur
*
6/8
75%
*
For these cotransfections the ratio of selection marker to expression vector (pTRE-Tight-Luc) was 1:20.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Linear Selection Markers (April 2003) Clontechniques XVIII(2):11.
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ