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ÀüÁ¦Ç°º¸±â
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NGS °ü·Ã
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mRNA-Seq (Single cell)
> Single cell RNA-seq¿¡ ÃÖÀûÀÎ SMART-Seq¢ç Single Cell Kit
Single cell Àü¿ëÀ¸·Î, ÃÖ°íÀÇ °¨µµ ¹× ¼º´ÉÀ¸·Î NGS ºÐ¼®
Single cell RNA-seq¿¡ ÃÖÀûÀÎ SMART-Seq¢ç Single Cell Kit
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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°¡°Ý
(ºÎ°¡¼¼º°µµ)
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»ç¿ëÀڸŴº¾ó
Clontech
R400750
SMART-Seq¢ç Single Cell PLUS Kit
48 ȸ
7,350,000¿ø
Clontech
R400751
SMART-Seq¢ç Single Cell PLUS Kit
96 ȸ
13,227,800¿ø
Clontech
634470
SMART-Seq¢ç Single Cell Kit
12 ȸ
1,634,400¿ø
2,043,000¿ø
05.02 ~ 06.30
Clontech
634471
SMART-Seq¢ç Single Cell Kit
48 ȸ
4,148,800¿ø
5,186,000¿ø
05.02 ~ 06.30
Clontech
634472
SMART-Seq¢ç Single Cell Kit
96 ȸ
7,576,000¿ø
9,470,000¿ø
05.02 ~ 06.30
Clontech
634473
SMART-Seq¢ç Single Cell Kit
480 ȸ
°¡°Ý¹®ÀÇ
05.02 ~ 06.30
Product index
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
[FAQ] RNA-seq
Cancer ¿¬±¸ °¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
SMARTer RNA-seqÀÇ HOT ..
mRNA-Seq (Single cell)
[Cancer Research] Canc..
Single cell-seq °¡À̵å
Single cell SMART-seq..
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
[Cancer Research] Canc..
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Cancer Research] Immu..
[Version 2] Human TCR ..
Human TCR a/b Profilin..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
Single cell-seq °¡À̵å
[FAQ] DNA-Seq
[Àû¿ë] Overview_ThruPL..
[Cancer Research] Canc..
DNA-seqÀÇ ´Ù¾çÇÑ ÃֽŠ..
ChIP-Seq / Meth-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[³í¹®] ThruPLEX¢ç for ..
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
[Cancer Research] Canc..
Single cell-seq °¡À̵å
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
[Cancer Research] HLA ..
[³í¹®] PicoPLEX¢ç ±â¼ú..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
[FAQ] Library index
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Citation
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) SMARTer¢ç Str..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
(Legacy) ThruPLEX¢ç Ta..
* 480ȸ ´ë¿ë·®ÀÇ SMART-Seq
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Single Cell Kit (Code 634473)ÀÇ ±¸¸Å¸¦ ¿øÇϽô ºÐÀº ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (Tel. 02-2081-2510)·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.
* PLUS kit´Â »ùÇ÷κÎÅÍ lllumina
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ºÐ¼®¿ë library Á¦ÀÛÀ» À§ÇÑ, library preparation kit¿Í single-use unique dual index (UDI)¸¦ Æ÷ÇÔÇϰí ÀÖ½À´Ï´Ù.
Robust, full-length chemistry
- SMART-seq2¸¦ Æ÷ÇÔÇÑ ±âÁ¸ÀÇ ¸ðµç single cell full-length ºÐ¼®¹ýÀ» ¶Ù¾î³Ñ´Â ¼º´É
Easy, plate-based workflow
- FACS µîÀ¸·Î ºÐ¸®ÇÑ single cell¿¡ ¹Ù·Î Àû¿ë °¡´É
Single-tube workflows -
cDNA ÇÕ¼ººÎÅÍ library Á¦ÀÛ °úÁ¤À» °¢°¢ single tube¿¡¼ ÁøÇàÇÒ ¼ö ÀÖ¾î, »ùÇà ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù ÃÖ¼ÒÈ
Unparalleled sensitivity and reproducibility
- ³ôÀº À¯ÀüÀÚ °ËÃâ È¿À²À» ÀÌ¿ëÇÑ ÃÖ°í ¼º´ÉÀÇ ´ÜÀÏ ¼¼Æ÷ RNA-seq (ƯÈ÷, RNA ÇÔ·®ÀÌ ¸Å¿ì ³·Àº ¼¼Æ÷ ¹× ÇÙ¿¡ Àû¿ë)
High library yield -
³ôÀº yieldÀÇ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ¾î, NovaSeq system°ú °°Àº high-throughput sequencer¿¡ Àû¿ëÇϰųª, ¿©·¯ Â÷·Ê ºÐ¼® °¡´É
Highly scalable workflow
- ´ë·® (High-throughput) ½ÇÇè Àû¿ë°¡´É, ÀÚµ¿È ±â±â Àû¿ë¿¡ ÃÖÀû
Á¦Ç°¼³¸í
SMART-Seq
¢ç
Single Cell Kit (SSsc)¿Í SMART-Seq
¢ç
Single Cell PLUS Kit (SSsc PLUS)´Â ³ôÀº ¹Î°¨µµ¿Í ÀçÇö¼ºÀ¸·Î single cell ȤÀº single nuclei¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. SSsc PLUS Á¦Ç°À» ÀÌ¿ëÇϸé, single cell¿¡¼ RNA¸¦ ÃßÃâÇÏÁö ¾Ê°í, ¹Ù·Î oligo(dT) primer¸¦ ÀÌ¿ëÇØ °íǰÁú full-length cDNA¸¦ Á¦Á¶ÇÑ ÈÄ, lllumina
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Ç÷§ÆûÀÇ library Á¦ÀÛ±îÁö ÁøÇàÇÒ ¼ö ÀÖ´Ù. Á¦Ç°À» ÀÌ¿ëÇÏ¿© 2 pg total RNA¿Í RNA ÇÔ·®ÀÌ ³·Àº °ÍÀ¸·Î ¾Ë·ÁÁ® ÀÖ´Â ¸»ÃÊÇ÷¾× ´ÜÇÙ¼¼Æ÷ (PBMCs)¿Í °°Àº single cell¿¡¼ ¿ì¼öÇÑ °á°ú¸¦ È®ÀÎÇÏ¿´´Ù.
º» Á¦Ç°Àº single cell ºÐ¼®À» À§ÇØ SMART (Switching Mechanism at 5' End of RNA Template) ±â¼úÀ» ¾÷±×·¹À̵åÇÏ¿© °³¹ßµÈ ½Ã½ºÅÛÀ¸·Î, ´õ ¸¹Àº read¿Í À¯ÀüÀÚ¸¦ °ËÃâÇÒ ¼ö ÀÖ´Ù. SSsc´Â ³ôÀº ÀçÇö¼º°ú gene-body coverage »Ó ¾Æ´Ï¶ó GC-rich Àü»çüµµ Á¤È®ÇÏ°Ô cDNA·Î ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
SMART-Seq
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Single Cell PLUS Kit (SSsc PLUS)¿¡ Æ÷ÇԵǾî ÀÖ´Â library preparation kit´Â enzymatic fragmentation¿Í stem-loop adaptor¸¦ ÇÔ²² »ç¿ëÇÏ¿©, 2½Ã°£ ³» single tube¿¡¼ purification °úÁ¤ ¾øÀÌ cDNA·ÎºÎÅÍ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. µû¶ó¼, »ùÇà ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù¸¦ ÃÖ¼ÒÈ ÇÒ ¼ö ÀÖ´Ù. ÀÌ °úÁ¤À» ÅëÇØ Á¦ÀÛµÈ library´Â SMART-Seq2 µî ±âÁ¸ÀÇ ¹æ¹ý ¹× ´ç»ç ±âÁ¸ Á¦Ç° (SMART-Seq
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v4)¿¡ ºñÇØ ÀçÇö¼ºÀÌ ³ô°í, ÈξÀ ¿ì¼öÇÒ »Ó ¾Æ´Ï¶ó, ³ôÀº yield·Î Á¦À۵Ǿî high-throughput sequencer¿¡ Àû¿ëÇϰųª, ¿©·¯ Â÷·Ê ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
±×¸² 1. SMART-Seq
¢ç
Single Cell Kit¿Í SMART-Seq2 ¹ý°úÀÇ °á°ú ºñ±³
SMART-Seq2À» ÀÌ¿ëÇßÀ» ¶§ º¸´Ù SMART-Seq
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Single Cell KitÀ» ÀÌ¿ëÇßÀ» ¶§, mitochondria À¯·¡ À¯ÀüÀڷκÎÅÍ ÈξÀ ´õ ³ôÀº mapped reads¿Í º¸´Ù ¸¹Àº À¯ÀüÀÚ°¡ °ËÃâµÊÀ» È®ÀÎÇÏ¿´´Ù.
Single cells from the lymphoblastoid cell line GM12878 were processed with the SMART-Seq
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Single Cell Kit (SSsc; 18 cells) or the Smart-seq2 method (Smart-seq2; 20 cells) using 19 cycles of PCR. RNA-seq libraries were generated and sequences analyzed (after normalizing all samples to 1.75 million paired-end reads).
(Panel A)
The read distribution was different between the two chemistries, with a drastically higher number of reads mapping to the mitochondrial genome (and therefore, fewer reads available for gene identification) with Smart-seq2 chemistry.
(Panel B)
More genes were detected in the cells processed with SSsc.
±×¸² 2.
SMART-Seq
¢ç
Single Cell Kit
¿Í ´ç»ç ±âÁ¸ Á¦Ç°ÀÎ SMART-Seq v4¿ÍÀÇ ºñ±³
´ç»ç ±âÁ¸ Á¦Ç°ÀÎ SMART-Seq
¢ç
v4À» ÀÌ¿ëÇßÀ» ¶§ º¸´Ù SMART-Seq
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Single Cell KitÀ» ÀÌ¿ëÇßÀ» ¶§,
´õ ³ôÀº cDNA yield¸¦ ¾ò¾ú°í, reads¿¡ °ü°è¾øÀÌ °ËÃâµÇ´Â À¯ÀüÀÚ ¼ö°¡ 60% ÀÌ»óÀÓÀ» È®ÀÎÇÏ¿´´Ù.
(Panel A)
12 single cells from lymphoblastoid cell line GM22601 were processed with SSv4 or SSsc using 19 cycles of PCR. All samples were normalized to 1.25 million paired-end reads. The cDNA yield generated with SSsc was drastically higher than that generated with SSv4.
(Panel B)
About 50 single PBMCs from one donor were processed with SSv4 or SSsc. About 60% more genes were detected in the cells processed with SSsc, regardless of the number of reads used for the analysis.
±×¸² 3.
SSsc PLUS
¸¦ ÀÌ¿ëÇÑ library¿¡¼ Nextera
¢ç
XTº¸´Ù ´õ ³ôÀº yield¿Í À¯ÀüÀÚ °ËÃâ
(Panel A)
The SMART-Seq
¢ç
Single Cell Kit was used to produce cDNA, in triplicate, from 10 pg of Mouse Brain Total RNA. Illumina
¢ç
-compatible libraries were then generated using either the SMART-Seq
¢ç
Library Prep Kit portion of the SSsc PLUS kit or Nextera
¢ç
XT. Libraries were sequenced on a NextSeq 500. The reads were normalized to 4M paired-end reads and analyzed as described in the technical note.
(Panel B)
Library yields obtained with the SSsc PLUS kit are higher than yields produced with Nextera
¢ç
XT.
(Panel C)
The SSsc PLUS kit also identified more genes compared to Nextera
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XT.
Àû¿ë
Single cell À¯·¡ full-length transcriptome ºÐ¼® (transcript isoforms, gene fusions, point mutations µî)À» À§ÇØ, cDNA ÇÕ¼º °úÁ¤¿¡¼ »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇØ ÇÕ¼ºÇÑ cDNA´Â SMART-Seq Library Prep kit (SSsc PLUS kit ³» Æ÷ÇÔ, º°µµ ±¸¸Å ºÒ°¡) ¸¦ ÀÌ¿ëÇÏ¿© Illumina
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ºÐ¼®¿ë sequencing library·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
Keyword : Single cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,full length,mRNA,mRNA-seq,cDNA,cDNA-seq,NGS,Next Generation Sequencing,SMART,,smart-seq,Rubicon,·çºñÄÜ
mRNA-Seq (Single cell)
[Cancer Research] Cancer single cell analysis
Single cell-seq °¡À̵å
Single cell SMART-seq¢ç ³í¹® ¸®½ºÆ®
Single cell Àü¿ëÀ¸·Î, ÃÖ°íÀÇ °¨µµ ¹× ¼º´ÉÀ¸..
Single cell RNA-seq¿¡ ÃÖÀûÀÎ SMART-Seq¢ç Single Cell Kit
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À» À§ÇÑ mRNA-Seq (SSv4)
NEW!
SSv4 ±â¹Ý, High-throughput mRNA-seq ºÐ¼®
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ÀÌ¿ëÇÑ ºÐ¼® (SSv4-HT)
3¡¯ Differential Expression (DE) ºÐ¼®À» High..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´ÜÀÇ ¼¿ ºÐ¼® (SSv4-DE)