TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö

Overview_ThruPLEX Application

-

Low Input DNA Library Preparation (For All Purpose)
ThruPLEX¢ç DNA-Seq Kit (Code R400674)
for fragmented dsDNA from any sample source (gDNA, cfDNA, ChIP DNA, cDNA, FFPE DNA)

High Performance
  • ±Ø¼Ò·®ÀÇ DNA ºÐ¼® : 50 pg ~ 50 ng DNA input
  • ´Ù¾çÇÑ »ùÇà Àû¿ë °¡´É : gDNA, cfDNA, ctDNA, ChIP DNA, FFPE DNA, cDNA
  • Compatible with major target enrichment platform
    (Agilent SureSelect¢ç, Roche Nimblegene¢ç, IDT ¿Ü)

Fast & Easy Protocol
  • 15ºÐ Hands-on-time : 15ºÐ ÀÛ¾÷ ½Ã°£, ÃÑ 2½Ã°£À̸é Illumina¢ç NGS Library Á¦ÀÛ ¿Ï·á
  • 3 Step in single tube (single-well) : Æ©ºê À̵¿ ¾øÀÌ ÇϳªÀÇ Æ©ºê¿¡¼­ 3¹øÀÇ ½ÇÇ轺ÅÜ
  • No intermediate purification : Beads purification °úÁ¤À» ÁÙ¿© ½±°í °£ÆíÇÏ°Ô

Applications
  • Whole Genome Sequencing (WGS)
  • Targeted DNA-Sequencing
  • RNA-Seq (cDNA-seq)
  • ChIP-Seq
  • Cell-free DNA-Seq (cfDNA-Seq, ctDNA-Seq)
  • FFPE DNA-Seq
  • Metagenome

ThruPLEX¢ç DNA-Seq KitÀ» ÀÌ¿ëÇÑ ´Ù¾çÇÑ ºÐ¼® »ç·Ê (Korean)
´Ù¾çÇÑ ½ÇÇè¿¡ ¸ðµÎ Àû¿ëÇÒ ¼ö ÀÖ´Â NGS Library Preparation Kit¸¦ ãÀ¸½Ã³ª¿ä?
»ùÇÃÀÌ ¼Ò·®ÀÌ¶ó¼­ ¾î¶»°Ô ºÐ¼®À» ÁøÇàÇÒ ¼ö ÀÖÀ»Áö °í¹Î ÁßÀ̽Ű¡¿ä?

Low input NGS Library Preparation¿¡ ThruPLEX¢ç DNA-Seq KitÀ» ÀÌ¿ëÇÑ Àû¿ë»ç·Ê¸¦ È®ÀÎÇϼ¼¿ä! ¿©·¯ºÐÀÇ ½ÇÇèÀÇ °ÝÀ» ³ô¿©µå¸³´Ï´Ù.

Applications

Àû¿ë »ç·Ê

µ¥ÀÌÅÍ Á¦°ø

ChIP-Seq

¼Ò·®ÀÇ ¼¼Æ÷ (10,000°³ÀÇ ¼¼Æ÷)·ÎºÎÅÍ ChIP-seq ºÐ¼® ±â¹ý È®¸³

Prof. Shirahige Katsuhiko (The University of Tokyo)

ChIP-Seq

¹ßÅé°³±¸¸® Ãʱ⠹è¾Æ¿¡¼­ ¹ßÇöÇÏ´Â ¹ß»ý Á¦¾î ÀÎÀÚ¿¡ ´ëÇÑ ChIP-Seq ºÐ¼® ¹æ¹ýÀÇ È®¸³

ÀÌÈ­Çבּ¸¼Ò (RIKEN)

ChIP-Seq
(CUT&RUN-seq)

Customer spotlight: profiling transcription factors with CUT&RUN sequencing

The lab of Shelley Berger, the University of Pennsylvania

Cell-free DNA

ThruPLEX¢ç DNA-Seq KitÀ» ÀÌ¿ëÇÑ Plasma cell-free DNA (cfDNA) ¼­¿­ ºÐ¼®

The University of Tsukuba

Metagenome

ThruPLEX¢ç DNA-Seq KitÀ» ÀÌ¿ëÇÏ¿© ¹Ì·®ÀÇ °Ëü·ÎºÎÅÍ Á¤È®ÇÑ ¹Ì»ý¹° Çؼ®

´ÙÄ«¶ó¹ÙÀÌ¿À

DMS-Seq

ThruPLEX¢ç DNA-Seq KitÀ» ÀÌ¿ëÇÑ Ãâ¾ÆÈ¿¸ðÀÇ DMS-Seq ºÐ¼® ±â¹ý

ÀÌÈ­Çבּ¸¼Ò(RIKEN), ¸¶ÀÌÅ©·Î¹ÙÀÌ¿È ¿¬±¸ÆÀ

* Àû¿ë»ç·Ê Á¦¸ñÀ» Ŭ¸¯ÇÏ½Ã¸é µ¥ÀÌÅ͸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ´Â ÆäÀÌÁö·Î À̵¿ÇÕ´Ï´Ù.


Technical Note of ThruPLEX¢ç DNA-Seq Kit (English)


Applications

Technical Note

DNA-Seq

Using Covaris microTUBE-15 to shear samples for ThruPLEX library preparation

ChIP-Seq

Library generation for ChIP-seq analysis of transcription factors with the ThruPLEX DNA-seq kit

ChIP-Seq

Low cell number ChIP-seq using the ThruPLEX DNA-seq kit as a tool for epigenetic profiling

FFPE DNA-Seq

A streamlined solution for generating high-quality NGS libraries from FFPE DNA samples

Exome-Seq

Low-input whole-exome sequencing using ThruPLEX technology and Agilent SureSelect Target Enrichment Systems

* Á¦¸ñÀ» Ŭ¸¯ÇÏ½Ã¸é µ¥ÀÌÅ͸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ´Â ÆäÀÌÁö·Î À̵¿ÇÕ´Ï´Ù.


[Âü°í] DNA-Seq Kit Á¦Ç°¸®½ºÆ®


* Á¦Ç°¸íÀ» Ŭ¸¯ÇÏ½Ã¸é »ó¼¼ ³»¿ëÀ» È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.

Code

Á¦Ç°¸í

¿ë·®

Input Sample

ÁÖ¿ä ¿ëµµ

R400674

ThruPLEX¢ç DNA-Seq Kit

24ȸ

50 pg - 50 ng DNA

* ¹ü¿ëÀûÀÎ Low input DNA-Seq
* ChIP DNA-Seq
* FFPE DNA-Seq
* Cell-free DNA-Seq

R400679

ThruPLEX¢ç Plasma-Seq Kit

24ȸ

1 ng - 30 ng cfDNA

* Cell-free DNA-Seq
* Plasma DNA Analysis
* Liquid Biopsy Analysis

R400584

ThruPLEX¢ç Tag-seq 6S (12) Kit

12ȸ

1 ng - 50 ng DNA

* NGS library with unique
   molecular tags
* Low frequency gene °ËÃâ

R300671

PicoPLEX¢ç Single Cell WGA Kit

24ȸ

15 pg - 50 pg DNA
1 - 10 cells

* Whole Genome Amplification
* Single Cell WGA
* Single Cell CNV °ËÃâ
* PGS/PGD (Array, PCR)

R300669

PicoPLEX¢ç Gold Single Cell DNA-seq Kit

24ȸ

15 pg - 30 pg DNA
1 - 5 cells

* Single cell DNA-Seq
* Single cell CNV, SNV °ËÃâ
* PGS/PGD (NGS)

R500653

microRNA-Seq Kit

16ȸ

100 ng - 1 ug RNA
2 ng - 200 ng miRNA

* microRNA-Specific NGS