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Recombinant Lentivirus 생산에 최적

TransIT®-Lenti Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Mirus
 MIR 6600
 TransIT®-Lenti Transfection Reagent
관련학술 구매하기 샘플신청  		1.5 ㎖
 가격문의   MIR 6600_transit-lenti-reagent-protocol.pdf
Mirus
 MIR 6603
 TransIT®-Lenti Transfection Reagent
관련학술 구매하기 		0.3 ㎖
 가격문의  
Mirus
 MIR 6604
 TransIT®-Lenti Transfection Reagent
관련학술 구매하기 		0.75 ㎖
 가격문의  
Mirus
 MIR 6605
 TransIT®-Lenti Transfection Reagent
관련학술 구매하기 		5 x 1.5 ㎖
 가격문의  
Mirus
 MIR 6606
 TransIT®-Lenti Transfection Reagent
관련학술 구매하기 		10 x 1.5 ㎖
 가격문의  
Mirus
 MIR 6620
 TransduceIT™ Transduction Reagent
관련학술 구매하기 		1 ㎖
 가격문의   MIR 6620_transduceit-reagent-pds.pdf



  • High Performance - Provide up to eight-fold higher functional titers
  • Simple Protocol - No media change required, single harvest
  • Animal Origin Free - Regulatory friendly
제품설명
TransIT®-Lenti Transfection Reagent는 adherent HEK 293T 세포에 packaging vector와 lentiviral vector를 효율적으로 co-transfection하여 recombinant lentivirus를 생산할 수 있는 transfection 시약이다.
TransduceIT™ Transduction Reagent는 hexadimethrine bromide 수용액으로 retroviral/lentiviral transduction 효율을 높이고자 할 때 사용한다.
보존 - 20 ℃
적용예
1. High Functional Titers with TransIT®-Lenti Transfection Reagent.


Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 2 μg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine™ 2000 (3:1), Lipofectamine™ 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 μg pDNA/well). The supernatant was harvested, filtered (0.45 μm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 μg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

2. High Transduction Efficiency with Unconcentrated Lentivirus Using TransIT®-Lenti.

(A) Lentivirus was produced with the TransIT®-Lenti Transfection Reagent (3:1, vol:wt) or Lipofectamine™ 2000 using the MISSION® vectors (pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION®). The supernatant was harvested, filtered (0.45 μm), and frozen. Lentivirus transductions were performed 5 days post-plating with iCell® Motor Neurons (Cellular Dynamics International). For bothTransIT-Lenti and Lipofectamine™ 2000, one microliter of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent the SEM of duplicate wells. (B) iCell® Motor Neurons were plated in Ibidy 35mm dishes and transduced withlentivirus produced using the TransIT®-Lenti Transfection Reagent and MISSION® vectors. Images were captured at 72 hours post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.

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