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Xfect™ Protein Transfection Reagent

제조사	제품코드	제품명	용량	가격 (부가세별도)	비고	사용자매뉴얼
Clontech	631323	Xfect Protein Transfection Reagent	30 rxns (Package)	899,000원
Clontech	631324	Xfect Protein Transfection Reagent	100 rxns (Package)	1,967,000원

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Overview
다양한 세포/범용적인 도입..
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- 광범위의 포유류 세포에 목적 단백질을 도입 가능
- 지극히 낮은 세포 독성
- 마우스 ES세포나 사람 조혈계 세포 등에도 고효율에 도입
- 심플한 프로토콜에 의해 2시간 이내에 도입 가능

제품설명

Xfect Protein Transfection Reagent는 Clontech이 독자적으로 개발한 단백질용 transfection 시약이다. 목적 단백질이 세포막을 통과 할 수 있도록 세포 투과성 펩티드(CPP；cell-penetrating peptide)를 채용해 목적 단백질을 고효율로 세포로 도입할 수 있다. 또한 세포 독성이 낮고 단백질의 활성을 유지한 상태로 세포에 도입할 수 있다(그림 1). 본 제품은 기존의 단백질 도입 효율이 낮다고 알려진 human 유래의 부유 세포나 mouse 유래 ES세포 등에도 효과적이다. Plasmid DNA를 이용한 유전자 도입시 plasmid DNA로부터 mRNA 전사를 거쳐 목적 단백질이 세포내에서 발현 될 때까지 12~24시간을 필요로 하지만, 본 제품을 이용하면 활성이 유지된 단백질을 1~2시간만에 세포에 직접 도입하는 것이 가능하다.

그림 1. Simple, rapid protein transfection with Xfect Protein Transfection Reagent. Xfect’s cell-penetrating activity enables proteins to be transported across membranes of mammalian cells.

그림 2. Xfect Protein Transfection Reagent delivers up to 8X more active b-galactosidase into HeLa cells than the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent or Product C was used to transfect Jurkat cells with various amount of b-galactosidase (b-gal) according to the manufacturers'recommended protocol. One hour post-transfection, the cells were assayed for b-gal activity using Clontech’s Luminescent b-galactosidase Detection Kit II (Cat. No. 631712). Xfect Protein Transfection Reagent displayed a far higher signal for b-gal than did Product C. Panel B. HeLa cells transfected with 4 μg of b-gal demonstrated higher efficiency and a higher amount of b-gal protein per cell when transfection was performed with Xfect Protein Transfection Reagent (middle panel) than with Product C (righthand panel).

그림 3. Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent yields higher transfection efficiencies than Product C across a broad range of mammalian cells, including a number of rodent and human cell lines, hard-to-transfect human suspension cells, and mouse embryonic stem cells. The cells were transfected with 4 ㎍ of recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express) using Xfect Protein Transfection Reagent or Product C according to the manufacturers' recommended protocol. Transfection efficiency was assessed by flow cytometry one hour post-transfection. Panel B. HeLa transfected with 5 ㎍ of rAcGFP1 using Product C or Xfect Protein Transfection Reagent.

그림 4. Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells. HeLa cells were seeded in 96-well plate (3 x 10⁴ cells per well) one day prior to transfection. The cells were transfected with 0.25 ㎍ of beta-galactosidase using Product P or Xfect Protein Transfection Reagent for 4 hours. A WST-1 proliferation assay was performed to assess cytotoxicity in the transfected samples relative to untreated HeLa cells.

구성품

· Xfect Protein Transfection Reagent
· Xfect Protein Buffer
· Deionized H₂O
· Beta-Galactosidase Control

Keyword :

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