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DNA/RNA Á¤Á¦ ¡¤ Ç¥½Ä
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Agarose Gel·Î ºÎÅÍ ÇÙ»ê ȸ¼ö
> Agarose Gel DNA Extraction Kit
Agarose Gel DNA Extraction Kit
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Takara
9762A
TaKaRa MiniBEST Agarose Gel DNA Extraction Kit
50 ȸ
99,000¿ø
Takara
9762B
TaKaRa MiniBEST Agarose Gel DNA Extraction Kit
200 ȸ (9762A x 4)
356,000¿ø
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TaKaRa MiniBEST Agarose Gel DNA Extraction Kit
Àº agarose gel¿¡¼ DNA ´ÜÆíÀ» ½Å¼ÓÇÏ°Ô Á¤Á¦Çϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù. º» Kit´Â agarose gelÀ» ¿ëÇؽÃÅ°´Â Àü¸Å buffer¿Í DNA fragment¸¦ Á¤Á¦Çϱâ À§ÇÑ columnÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù. Buffer´Â °ÇÑ ¹öÆÛ Æ¯Â¡À» °®°í ÀÖÀ¸¸ç Spin column membrane¿¡ ÃÖÀûÈµÈ pH°ªÀ» °®´ÂÁö ½±°Ô È®ÀÎÇÒ ¼ö ÀÖ´Â pH indicator¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. Ãß°¡·Î buffer´Â °¡¿¾øÀÌ ½Ç¿Â(15~25¡É)¿¡¼ gelÀ» ºü¸£°Ô ³ìÀÏ ¼ö ÀÖ´Ù. 20ºÐÀ̳» ½ÇÇè Àü °úÁ¤À» ¼öÇàÇÒ ¼ö ÀÖ´Ù. Spin ColumnÀº ÃÖ´ë 20 ¥ìg±îÁö DNA¸¦ °áÇÕ½Ãų ¼ö ÀÖ°í 50 bp~20 kbpÀ̳»ÀÇ DNA ´ÜÆíÀ» È¿°úÀûÀ¸·Î ȸ¼öÇÒ ¼ö ÀÖ´Ù. ȸ¼öÀ²Àº ¾à 50~80% Á¤µµµÈ´Ù. ±×·¯³ª DNA ´ÜÆíÀÌ 20~50 kbp»çÀ̸é ȸ¼öÀ²Àº ´õ ¶³¾îÁú ¼ö ÀÖ´Ù. º» kitÀ¸·Î Á¤Á¦ÇÑ DNA´Â ligation, PCR, sequencing°ú °°Àº ¸ðµç Á¾·ùÀÇ ºÐÀÚ»ý¹°ÇÐ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë
1¹ÝÀÀ ´ç 300 mgÀÇ agarose gel(1% ³óµµÀÇ agarose ±âÁØ)À» ó¸®ÇÒ ¼ö ÀÖ´Ù.
º» KitÀº Reagent Set¿Í Column SetÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
Reagent Set
Buffer GM
£ª1
50 ml
Buffer WB
£ª2
24 ml
Elution Buffer
2ml x 2
£ª1
: Contain strong denaturant. Be careful to avoid contacting with eyes and skin. In the case of such contact, wash immediately with plenty
water and seek medical advice.
£ª2
: Before the first use of the kit, add 56 ml of 100% ethanol to Buffer WB.
Column Set
Spin Columns
50
Collection tubes
50
¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% Ethanol
2. Sterilized water or Tris-HCl (pH8.0)
3. 3 M sodium acetate (pH5.2)
º¸°ü ¹× ¿î¼Û
1. The kit can be stored at room temperature (15 - 25¡É).
2. The kit is shipped at room temperature (15 - 25¡É).
3. Precipitate may occur in the Buffer GM under low temperature. Please warm with a 37¡É to dissolve and use after returning to room
temperature.
Keyword :
Agarose Gel·Î ºÎÅÍ ÇÙ»ê ȸ¼ö
Agarose Gel DNA Extraction Kit
³î¶ó¿ï Á¤µµ·Î °£Æí, ½Å¼ÓÇÑ DNA ´ÜÆí ȸ¼ö ½Ã..
FlashGel¢â System for DNA Recovery
Agarose Gel·Î ºÎÅÍ ÇÙ»ê ȸ¼ö chip
TaKaRa RECOCHIP