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siRNA 도입 용 시약

TransIT-TKO Transfection Reagent

제조사	제품코드	제품명	용량	가격 (부가세별도)	비고
Mirus	MIR 2154	TransIT-TKO Transfection Reagent	0.4 ml	가격문의	11.01 ~ 12.27
Mirus	MIR 2150	TransIT-TKO Transfection Reagent	1.5 ml	가격문의	11.01 ~ 12.27
Mirus	MIR 2155	TransIT-TKO Transfection Reagent	1.5 ml×5	가격문의	11.01 ~ 12.27
Mirus	MIR 2156	TransIT-TKO Transfection Reagent	1.5 ml×10	가격문의	11.01 ~ 12.27

Product index

Overview
다양한 세포/범용적인 도입..
효율증대를 위한 특수도입시..
세포에 따른 효율적인 유전..
Electroporation 관련
- Ingenio® solution & k..
- Ingenio® cuvette, 소..
도입물질에 따른 도입시약
in-vivo delivery 시스템
- TransIT Hydrodynamic D..
독성 제거 - Transfection ..
- MiraCLEAN® Endotoxin ..
siRNA Transfection 시약
siRNA 발현 · 분석
- pSINsi vector 시리즈
- Synthetic siRNA Quanti..
small RNA Cloning
RNAi 관련 Kit
- 14-30 ssRNA Ladder Mar..
- siRNA Ladder Marker

광범위한 세포에 siRNA를 고효율로 도입 가능
낮은 세포 독성으로 배지교환 불필요
높은 Knock-down 효율로, 재현성 높은 실험 결과

Technical Report: High Efficiency siRNA Delivery In Vitro (pdf) (클릭)
Publication: Delivery of small interfering RNA to mammalian cells in culture (클릭)

제품설명

TransIT-TKO는 짧은 dsRNA (siRNA)을 진핵세포에 transfection하기 위해서 최적화된 시약이다. 세포에 대한 독성이 낮고, 높은 효율로 siRNA를 도입할 수 있으므로 표적 유전자의 발현을 Knock-down 하는 실험에 사용할 수 있다. 또한 혈청을 포함하는 배지에도 transfection이 가능하며, 배지교환이 불필요하므로 시간을 절약할 수 있다.
24 well plate의 경우 본 시약 1 ml으로 약 500회분의 transfection을 실시할 수 있다. 그리고 TransIT-LT1 (Code MIR 2304/MIR 2300)과 함께 이용하면 siRNA 와 plasmid DNA의 co-transfection이 가능하다.

Figure 1. High Efficiency Knockdown and Low Toxicity Using TransIT-TKO^® Reagent in HeLa Cells. Firefly and sea pansy luciferase reporter vectors were co-transfected into HeLa cells using TransIT^®-LT1 Reagent. Cells were incubated for at least 4 hours, split into 24-well plates, allowed to adhere and transfected with 25 nM of either a non-targeting siRNA or an anti-firefly luciferase siRNA using the indicated reagents with the volumes noted beneath each well. Luciferase expression, normalized to non-targeting siRNA control (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.

Figure 2. Delivery of Fluorescently-Labeled siRNA Using TransIT-TKO^® Transfection Reagent. HeLa (70% confluence) cells in 12-well plates were transfected with TransIT-TKO^® Transfection Reagent (3 μl/well) and Label IT^® siRNA Tracker Fluorescein-labeled siRNA duplexes (GREEN, 50 nM final concentration in the well). The cells were incubated 24 hours post-transfection then fixed and counterstained with TO-PRO^®-3 (nuclei, BLUE) (Life Technologies) and Alexa Fluor^® 546 Phalloidin (actin, RED) (Life Technologies). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope.

표 1. TransIT-TKO를 이용한 목적 유전자 Knock-down 효율

세포	Target Gene	Knock-Down efficiency (qRT-PCR)
BNL CL.2 (mouse liver)	MAPK1	80 %
BNL CL.2 (mouse liver)	MAPK3	83 %
HeLa (human cervix)	Lamin A / C	80 %
HeLa (human cervix)	GAPDH	80 %
Hepa1c1c7 (mouse liver)	MAPK1	80 %
	MAPK3	75 %
	MEK1	75 %
	PTEN	80 %
HepG2 (human liver)	MAPK1	80 %
NIH 3T3-L1	MAPK1	70 %
NIH 3T3-L1	MAPK3	70 %
Secondary Human Astrocytes	Lamin A / C	80 %
Primary Mouse Hepatocytes	ABC A1	70 %
Primary Mouse Hepatocytes	Lamin A / C	81 %

보존 4℃

Keyword : V2154,MIR2154,siRNA,V2150,MIR2150,V2155,MIR2155,V2156,MIR2156

siRNA Transfection 시약

도입 효율 확인 용 표식 Control

Label IT Delivery Control

Label IT siRNA Tracker Intracellular Localization Kit

Mouse 생체 내 세포 용 siRNA 도입 시약

TransIT-QR Hydrodynamic Delivery Solution

siRNA 도입 용 시약

TransIT-siQUEST Transfection Reagent

siRNA 도입 용 시약

TransIT-TKO Transfection Reagent

Plasmid DNA와 siRNA/miRNA를 효과적으로 deliv..

TransIT-X2 Dynamic Delivery System