• DNA나 RNA 주형의 변형 - nucleic acid의 amine 그룹에 직접 공유 결합
• One-step Chemical Method - nucleic acid modification의 농도(밀도)를 일관되고 쉽고 조절
• Covalent Mechanism - 영구적이고 비파괴적인 nucleic acid modification으로 protein이나 peptide와 결합하거나 유리표면이나 bead 또는 plate에 부착 가능

Kit Components

Label IT Reagent, Label IT Reconstitution Solution, Labeling Buffer, Solutions and G50 Spin Columns.



Figure 1. The Label IT® Nucleic Acid Modifying Kit. The Label IT nucleic acid modifying reagent is composed of three regions: the reactive NH2 modifying group (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT modifying reagent to any reactive heteroatom within nucleic acids. Attachment of the Label IT modifying reagent to nucleic acids does not alter the structure of the nucleic acids or affect downstream hybridization performance. Once labeled, the nucleic acids can be coupled to reactive substrates of other chemical entities through the added NH2 reactive groups.



Figure 2. Demonstration of the Functionality of Amine Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT Amine Reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Five μg of the modified DNA, in triplicate, was reacted with 10 mM NHS ester TM-Rhodamine and 100 mM NaHCO3 (pH 8 - 8.5) for 1 hour at room temperature in the dark. The DNA was again ethanol precipitated, washed extensively, and assayed for labeling efficiency.