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TransIT-PRO¢ç Transfection Reagent & Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 5740
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 ml
°¡°Ý¹®ÀÇ   transit-pro-transfection-reagent-protocol.pdf
Mirus
MIR 5750
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
°¡°Ý¹®ÀÇ  



Á¦Ç°¼³¸í
Trans IT-PRO Transfection Reagent´Â ºÎÀ¯ ¼¼Æ÷ÀÎ 293 cell°ú CHO cell¿¡¼­ ´Ü¹éÁúÀ» »ý»êÇϵµ·Ï °³¹ßµÈ animal-origin free DNA transfection ½Ã¾àÀÌ´Ù. º» Á¦Ç°Àº ´Ù¾çÇÑ ¹èÁö¿¡ Àû¿ë °¡´ÉÇϸç, transfection ÈÄ ¹èÁö ±³È¯ÀÌ ÇÊ¿äÇÏÁö ¾Ê°í transient transfectionÀ̳ª stable transfection ¸ðµÎ¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
  • Suspension CHO¿Í 293 cell¿¡¼­ °í¼öÀ² ´Ü¹éÁú ¹ßÇöÀ» À§ÇÑ transfection reagent
  • ´Ù¾çÇÑ CHO ¹èÁö Á¶¼º¿¡¼­ ȣȯ¼º ¿ì¼ö
  • ÀçÇö¼ºÀÌ ³ôÀº ´Ü¹éÁú ¹ßÇöÀ» ½ÇÇö
  • CHO cell line ´Ü¹éÁú »ý»êÀ» À§ÇÑ ÃÖÀûȸµÈ transfection & ¹èÁö ½Ã½ºÅÛ - CHOgro¢ç High Yield Expression System
Transfection ÈÄ ½Ã°£°æ°ú¿¡ µû¸¥ ¹ßÇöÀ² ºñ±³

±×¸² 1. Achieve High Protein Yields Using TransIT-PRO¢ç Transfection Kit in Suspension 293 Cells. Ten different secreted (non-antibody) proteins were transiently expressed in FreeStyle¢â 293-F cells (Life Technologies) using TransIT-PRO (1.5:1) or 293fectin¢â (Life Technologies, 2:1) transfection reagents according to manufacturer¡¯s protocol. Cells were grown in FreeStyle¢â 293 Expression Medium at transfected at a density of 1 x 106 cells/ml. The scale of the transfection for each protein varied between 1-6 L of culture. 
º¸Á¸ : -20¡É
°ü·Ã Á¦Ç°
- Suspension CHO cellÀ» »ç¿ëÇÑ Ç×ü/´Ü¹éÁú ´ë·® ¹ßÇö ½Ã½ºÅÛ: CHOgro¢ç High Yield Expression System
- Ç×ü/´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛ ±¸ÃàÀ» À§ÇÑ HTS cloning: In-Fusion¢ç Snap Assembly Master Mix
- Plate ŸÀÔÀÇ competent cell: Stellar¢â Competent Cells (96 well plate)
- Error ¾ø´Â insert PCRÀ» À§ÇÑ high fidelity PCR È¿¼Ò: PrimeSTAR¢ç Max DNA Polymerase Ver.2

Keyword : V5700,MIR5700,V5760,MIR5760

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