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TransIT
®-AAViator Transfection System (Code MIR 73750)의
대용량 제품 (15L cell culture용)은 다카라코리아 고객지원 (02-2081-2510,
support@takara.co.kr)로 문의 바랍니다.
- 높은 감염활성을 가지는 고품질의 AAV 생산에 최적
- HEK293 세포 배양 시스템에 최적 (adherent 및 suspension 배양 모두 적용)
- 연구용 시약부터 전임상 단계, 임상용 GMP grade까지 라인업
제품 설명
TransIT®- AAViator Transfection System은 Mirus의 입증된 polymer, lipid technology을 이용하여 개발된 AAV 생산을 위한 맞춤 시스템이다. 본 제품을 사용하면, 타사의 Polymer 단일 시약에 비해 AAV 생산량을 증가시킬 수 있고, 쉽게 scale up 할 수 있으며, 플라스미드 DNA 사용량을 최대 50% 아낄 수 있는 장점이 있다.
TransIT®- AAViator Transfection System은 새로운 TransIT®-AAViator Transfection Reagent와 RevIT™ AAV Enhancer로 구성되어 있으며, 함께 사용하여 AAV를 최대로 생산할 수 있다.
그림1. 타사 제품 대비 높은 AAV 생산율을 보이는 TransIT®-AAViator System
Viral Production 2.0 cells grown in Viral Production Medium were transfected with pAAV-WPRE-GFP, pAAV-Helper and pAAV-RepCap (AAV5, AAV8, AAV9) at a 1:1:1 plasmid concentration ratio with the following reagents: TransIT®-AAViator Transfection reagent (1.25:1,vol:wt), Polymer F (1:1) , or Polymer P (1:1). RevIT™-AAV Enhancer (1 μl/mL) was added at the time of transfection. All AAV serotypes were harvested at 72 hours post-transfection using chemical lysis. Genome titers were determined via dPCR using primers and a probe targeting the CMV promoter region. Total assembled capsids were determined using the AAV9, AAV8 or AAV5 Titration ELISA Kits, and the Percent Full Capsids were determined by dividing the number of genome copies by total assembled capsids for each condition. The error bars represent the standard deviation of duplicate wells.)
그림2. Plasmid DNA 사용량에 관계없이 높은 수율로 AAV를 생산하는TransIT-AAViator System
Viral Production 2.0 cells grown in Viral Production Medium were transfected with pAAV-WPRE-GFP, pAAV-Helper and pAAV-RepCap at a 1:1:1 plasmid concentration ratio with TransIT®-AAViator Transfection reagent (1.25:1,vol:wt). RevIT™-AAV Enhancer (1 ㎕/㎖) was added at the time of transfection. AAV was harvested at 72 hours post-transfection using chemical lysis. Genome titers were determined via dPCR using primers and a probe targeting the CMV promoter region. The error bars represent the standard deviation of duplicate wells.
보존
TransIT®-AAViator Transfection Reagent / RevIT™ AAV Enhancer: -10~-30℃ 보존
관련제품
