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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > Hepatocyte > iPS Cell to Hepatocyte Differentiation System
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iPS Cell to Hepatocyte Differentiation System

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Cellartis
Y30035
Cellartis Definitive Endoderm Diff Kit with DEF-CS
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
(Package)
°¡°Ý¹®ÀÇ   x32557, x32561
Cellartis
Y30050
Cellartis Hepatocyte Diff Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 kit
(Package)
°¡°Ý¹®ÀÇ   x32563
Cellartis
Y30051
Cellartis Hepatocyte Maintenance Medium
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ml x 2
°¡°Ý¹®ÀÇ   x32564
Cellartis
Y30055
Cellartis iPS Cell to Hepatocyte Differentiation System
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 kit
(Package)
°¡°Ý¹®ÀÇ   x32569


  • Àΰ£ ¸¸´ÉÁٱ⼼Æ÷·ÎºÎÅÍ °£¼¼Æ÷·Î ºÐÈ­ À¯µµ °¡´É
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  • ³ôÀº ºÐÈ­ À¯µµ È¿À²À» ÅëÇÏ¿© ±ÕÁúµµ°¡ ³ôÀº °£¼¼Æ÷ (90 % ÀÌ»ó) À¯µµ
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Cellartis iPS Cell to Hepatocyte Differentiation SystemÀº 3ÁÖ¸¸¿¡ iPS ¼¼Æ÷·ÎºÎÅÍ °£¼¼Æ÷·Î ºÐÈ­ À¯µµ ÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº ºÐÈ­ À¯µµÀÇ °¢ ´Ü°è¿¡¼­ ÇÊ¿äÇÑ ¹èÁö¿Í ready-to-use »óÅÂÀÇ ÄÚÆÿë¾×À» Æ÷ÇÔÇÏ°í ÀÖ´Ù. 3 ¡¿ 106°³ÀÇ iPS ¼¼Æ÷·ÎºÎÅÍ 5 ¡¿ 106 °³ (50 cm2 ¸éÀû »ó´ç)ÀÇ °£¼¼Æ÷¸¦ ºÐÈ­½Ãų ¼ö ÀÖ´Ù.
ºÐÈ­µÈ °£¼¼Æ÷´Â ÁÖ¿äÇÑ °£ ¸¶Ä¿ À¯ÀüÀÚ¸¦ ¹ßÇöÇÏ°í, »ç¿ëÇÑ iPS ¼¼Æ÷¿¡ ÀÇÁ¸ÇÑ CYP È°¼ºÀ» °¡Áö°í ÀÖ´Ù. °£¼¼Æ÷·Î ºÐÈ­ À¯µµ °¡´ÉÇÑ Àΰ£ iPS ¼¼Æ÷ (ChiPSC18)´Â º°µµ·Î ÆǸÅÇÏ°í ÀÖ´Ù.
ºÐÈ­ À¯µµ¸¦ ÅëÇؼ­ Á¦Á¶µÈ °£¼¼Æ÷´Â ½ÃÁß¿¡ ÆǸŵǰí ÀÖ´Â µ¿°á °£¼¼Æ÷º¸´Ù Àå±â°£ ¹è¾çÀÌ °¡´ÉÇϸç, Àå±â µ¶¼º ½ÃÇèÀ̳ª ¹ÙÀÌ·¯½º °¨¿° ½ÃÇè¿¡µµ ÀûÇÕÇÏ´Ù.

[Àΰ£ iPS ¼¼Æ÷·ÎºÎÅÍ °£¼¼Æ÷·ÎÀÇ ºÐÈ­ À¯µµ È帧]
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º» Á¦Ç°Àº Cellartis Definitive Endoderm Diff Kit with DEF-CS (Code Y30035)¿Í Cellartis Hepatocyte Diff Kit (Code Y30050)·Î ±¸¼ºµÇ¾î ÀÖ´Ù.

Cellartis Definitive Endoderm Diff Kit with DEF-CS (Code Y30035)

¡¤ Differentiation media 6 Á¾

¡¤ Coating ¿ë¾× 1 °³

¡¤ Cellartis DEF-CS 100 Culture System

Cellartis DEF-CS Basal Medium
100 ml
Cellartis DEF-CS COAT-1
800 ¥ìl
: Ç÷¹ÀÌÆ® ÄÚÆà (6 well plate ±âÁØ ¾à 16 well »ç¿ë°¡´É)
DEF-CS GF-1
300 ¥ìl
¹èÁö÷°¡Á¦(Growth factor)
DEF-CS GF-2
100 ¥ìl
DEF-CS GF-3
40 ¥ìl

Cellartis Hepatocyte Diff Kit (Code Y30050)
¡¤ Hepatocyte Thawing and Seeing Medium (1)
25 ml
¡¤ Hepatocyte Progenitor Medium (2)
50 ml
¡¤ Hepatocyte Maturation Medium Base (3A)
24 ml ¡¿ 2 ea
¡¤ Hepatocyte Maturation Medium Supplement (3B)
2.6 ml
¡¤ Hepatocyte Maintenance Medium (4)
50ml ¡¿ 4 ea *
¡¤ Hepatocyte Coating
7.5 ml
*11ÀÏ°£ ¹è¾ç À¯Áö ÇÒ ¼ö ÀÖ´Â ¿ë·®ÀÔ´Ï´Ù. ±× ±â°£ ÀÌ»óÀÇ ¹è¾ç¿¡´Â º°µµÀÇ Cellartis Hepatocyte Maintenance Medium (Code Y30051)¸¦ ÀÌ¿ëÇØ ÁֽʽÿÀ.
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¡¤ Cellartis Definitive Endoderm Diff Kit with DEF-CS (Code Y30035)
   ¡¤ Differentiation media : 4 ¡É
   ¡¤ Coating : -20 ¡É
   ¡¤ Cellartis DEF-CS 100 Culture System
      DEF-CS Basal Medium (100 ml) : 4 ¡É
      DEF-CS COAT-1 : -20 ¡É
      DEF-CS GF-1 GF-2, GF-3 : -20 ¡É
¡¤ Cellartis Hepatocyte Diff Kit (Code Y30050) : - 20 ¡É


±×¸² 1. °£¼¼Æ÷·ÎÀÇ ºÐÈ­ À¯µµ°úÁ¤½Ã ¸é¿ªÁ¶Á÷¿°»ö °á°ú
Cellartis Definitive Endoderm ChiPSC18 cells°ú Cellartis Hepatocyte Diff KitÀ» »ç¿ëÇÏ¿© ºÐÈ­ À¯µµÇÑ °£¼¼Æ÷¸¦ 14ÀÏ°ú 21ÀÏ¿¡ Ãʱ⠰£ ¸¶Ä¿ÀÎ HNF4¥á (red, nuclear)¿Í CK18 (green)À¸·Î ¿°»öÇß´Ù. 28ÀÏ¿¡´Â °£ ƯÀÌÀû ¸¶Ä¿ÀÎ CYP3A (red, cytoplasmic)¿Í Albumin (green)ÀÇ ¹ßÇöÀÌ Áõ°¡µÇ°í ÀÖ¾î °£¼¼Æ÷°¡ ¼º¼÷µÇ°í ÀÖÀ½À» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù. ¼¼Æ÷ÇÙÀº DAPI (blue)·Î ¿°»öµÇ¾ú´Ù.


±×¸² 2. °£¼¼Æ÷·ÎÀÇ ºÐÈ­ À¯µµ °úÁ¤½Ã À¯ÀüÀÚ ¹ßÇö ÇÁ·ÎÆÄÀϸµ
Cellartis Definitive Endoderm ChiPSC18 cells°ú Cellartis Hepatocyte Diff KitÀ» »ç¿ëÇÏ¿© ºÐÈ­ À¯µµÇÑ ¼¼Æ÷¿¡ ´ëÇØ °£¸¶Ä¿ À¯ÀüÀÚ ¹× CYP À¯ÀüÀÚÀÇ mRNA ¹ßÇö·®À» RT-qPCR·Î ºÐ¼®Çß´Ù. ±× °á°ú 11ÀÏ°ºÎÅÍ 32ÀÏ°±îÁö °£¼¼Æ÷·Î ºÐÈ­µÇ¾îÀÖ´Â °ÍÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.


±×¸² 3. °£¼¼Æ÷·ÎÀÇ ºÐÈ­ À¯µµ °úÁ¤½Ã CYP È°¼ºÀÇ º¯È­
Cellartis Definitive Endoderm ChiPSC18 cells°ú Cellartis Hepatocyte Diff KitÀ» »ç¿ëÇÏ¿© ºÐÈ­ À¯µµÇÑ ¼¼Æ÷¿¡ ´ëÇØ CYP È°¼ºÀ» ÃøÁ¤Çß´Ù. ±× °á°ú 21ÀÏ°ºÎÅÍ 32ÀÏ°±îÁö CYP1A, CYP3, CYP2C9ÀÇ È°¼ºÀÌ È®ÀεǾú´Ù. CYP1AÀº acetaminophen, CYP3A´Â 1-OH-Midazolam, CYP2C9´Â 4-OH-DiclofenacÀÇ »ý¼ºÀ» ÃøÁ¤ÇÏ¿´´Ù.
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1. One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent
   Stem Cells

   Asplund A, et. al. Stem Cell Rev. 2016 Feb;12(1):90-104
°ü·ÃÁ¦Ç°
- Human albumin ÃøÁ¤¿ë pre-coated EIA kit: Human Albumin EIA Kit
- Mouse albumin ÃøÁ¤¿ë pre-coated EIA kit: Mouse Albumin EIA Kit

Keyword : °£¼¼Æ÷,Áٱ⼼Æ÷ºÐÈ­,Áٱ⼼Æ÷À¯µµ,Hepatocyte,°£µ¶¼º,toxicitytest,°£¼¼Æ÷ºÐÈ­,HepaRG,HepG2