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Home > ÀüÁ¦Ç°º¸±â > ¼¼Æ÷¤ýÀ¯ÀüÀÚ Ä¡·á ¿¬±¸ > T cell therapy ¿¬±¸ > LymphoONE¢ç T-Cell Expansion Xeno-Free Medium
T cell ¹è¾ç¹èÁö (Xeno-Free, Serum-Free)

LymphoONE¢ç T-Cell Expansion Xeno-Free Medium

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
WK552S
LymphoONE¢ç T-Cell Expansion Xeno-Free Medium, 1L Bottle
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 §¢
°¡°Ý¹®ÀÇ   WK552S_e.v1903.pdf, [Takara] LymphoONE T-Cell Expansion XF Medium »ç¿ë¹ý (plate¹è¾ç½Ã).pdf
Takara
WK551S
GT-T551 Culture medium, 1L Bottle
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 §¢
°¡°Ý¹®ÀÇ   WK551S_e.v1602.pdf



  • Anti-CD3 antibodyÀÇ Àڱذú IL-2 Á¸ÀçÇÏ¿¡¼­ T cellÀÇ ³ôÀº Áõ½ÄÀ² À¯Áö
  • Ç÷ûÀ» ÷°¡ÇÏÁö ¾Ê¾Æµµ ³ôÀº ¼ºÀ强 À¯Áö °¡´É
  • µ¿¹° À¯·¡ ¼ººÐÀ» Æ÷ÇÔÇÏÁö ¾Ê´Â Xeno-Free ¹èÁö
    (Human serum albumin (pharmaceutical-grade) ¹× recombinant human insulin Æ÷ÇÔ)
  • Endotoxin °Ë»ç¿Ï·á (endotoxin 0.3 EU/§¢ ÀÌÇÏ) ¹× mycoplasma, ¹«±Õ ½ÃÇè ¿Ï·á

£ªLymphoONE¢â T-Cell Expansion Xeno-Free Medium (Code WK552S)´Â ¹èÁö ¼ººÐÀÇ ÃÖÀûÈ­¸¦ ÅëÇØ Ç÷û, Ç÷ÀåÀ» ÷°¡ÇÏÁö ¾Ê¾Æµµ ±âÁ¸Á¦Ç°ÀÎ GT-T551 Culture medium (Code WK551S)º¸´Ù T cell Áõ½ÄÀ²À» ³ôÀÎ Á¦Ç°ÀÔ´Ï´Ù.
Á¦Ç°¼³¸í
LymphoONE¢â T-Cell Expansion Xeno-Free MediumÀº human T cellÀÇ È®´ë ¹è¾ç»Ó¸¸ ¾Æ´Ï¶ó, retrovirus vector, lentivirus vector¸¦ T cell¿¡ À¯ÀüÀÚ µµÀÔÇÏ´Â °æ¿ì¿¡µµ ÃÖÀûÀÇ ¹èÁöÀÌ´Ù. £¨¹èÁö¿¡´Â Human serum albumin (pharmaceutical-grade) ¹× recombinant human insulin ÀÌ¿ÜÀÇ ´Ü¹éÁúÀº ÷°¡µÇÁö ¾Ê¾Ò°í, cell ¹è¾ç¿¡ ±×´ë·Î »ç¿ë °¡´ÉÇϸç Ç÷û, Ç÷ÀåÀ» ÷°¡ÇÏ´Â °æ¿ì ¼¼Æ÷ Áõ½Ä·üÀ» ´õ ³ôÀÏ ¼ö ÀÖ´Ù.
T cellÀº Anti-CD3 mAb GMP grade (Code T210) stimulation ÈÄ, cytokine (IL-2, IL-5 µî)À» Æ÷ÇÔÇÑ LymphoONE¢â T-Cell Expansion Xeno-Free MediumÀ» ÀÌ¿ëÇÏ¿© ¹è¾çÇÔÀ¸·Î½á Áõ½Ä½Ãų ¼ö ÀÖ´Ù. Anti-CD3 antibody¸¦ ÀÌ¿ëÇÑ stimulation ½Ã RetroNectin¢çÀ» µ¿½Ã¿¡ »ç¿ëÇÔÀ¸·Î½á T cell È®´ë¹è¾ç È¿À²À» ³ôÀÏ ¼ö ÀÖ´Ù.
ÀÌ¿Í °°ÀÌ È®´ë ¹è¾çµÈ T cell Áý´Ü¿¡¼­´Â Anti-CD3 antibody ´Üµ¶À¸·Î »ç¿ëÇßÀ» ¶§ º¸´Ù naive T cellÀÇ ºñÀ²À̳ôÀº °ÍÀ¸·Î º¸°íµÇ°í ÀÖ´Ù (Âü°í ¹®Çå: Hosoi et al. 2014).
CellÀ» È®´ë ¹è¾çÇϱâ À§ÇÑ ¹è¾ç ¿ë±â·Î´Â ¼¼Æ÷ ¹è¾ç¿ë Çöó½ºÅ©, plate ¿Ü¿¡ °¡½º Åõ°ú¼º culture bagÀÎ CultiLife¢â 215 Culture bag (Code FU0005)¸¦ ÀÌ¿ëÇÑ ¿ÏÀü Æó¼â°è ¹è¾çÀÌ °¡´ÉÇÏ´Ù.
RetroNectin¢çÀ» ÀÌ¿ëÇÑ T cell È®´ë¹è¾ç ¹æ¹ý ¹× ¿¹´Â ¾Æ·¡ÀÇ ÆäÀÌÁö¸¦ Âü°í ¹Ù¶ø´Ï´Ù.


[LymphoONE¢â T-Cell Expansion Xeno-Free Medium]
T cell È®´ë¹è¾ç »ç¿ë ¿¹
1. T cellÀÇ È®´ë ¹è¾çÀÇ ¹èÁö ¼º´ÉÀÇ ºñ±³
[¹æ¹ý]
2¸íÀÇ °ø¿©ÀÚ*¿¡¼­ äÃëÇÑ Ç÷¾×¿¡¼­ Peripheral blood mononuclear cells (PBMC) ¹× Ç÷ÀåÀ» ºÐ¸®ÇÏ¿©, PBMC¸¦ Anti-CD3 mAb°¡ Æ÷ÇÔµÈ CultiLife 215 Culture bag¿¡¼­ stimulationÇϸç 4ÀÏ°£ ¹è¾ç ÈÄ, IL-2¸¦ Æ÷ÇÔÇÑ ´Ù¾çÇÑ ¸²ÇÁ±¸ ¹è¾ç¿ë ¹èÁö¿¡¼­ Àû´çÈ÷ Èñ¼®Çϸç 10ÀÏ°£ ¹è¾çÇÏ¿´´Ù.
* ½ÇÇè³»¿ëÀ» °íÁö ÈÄ µ¿ÀǸ¦ ¾ò¾î »ùÇÃÀ» äÃëÇÏ¿´½À´Ï´Ù.

[°á°ú]


LymphoONE¢â T-Cell Expansion Xeno-Free MediumÀº Ç÷û (human AB serum) À» ÷°¡ÇÏÁö ¾ÊÀº ¹è¾ç Á¶°Ç¿¡¼­µµ ¸Å¿ì ³ôÀº È®´ë¹è¾ç·üÀ» º¸¿´°í, ±âÁ¸ Á¦Ç°º¸´Ùµµ PBMC Áõ½Ä ´É·ÂÀÌ ¿ì¼öÇÑ °ÍÀ¸·Î È®ÀεǾú´Ù.

2. RetroNectin¢ç co-stimulation¿¡ ÀÇÇÑ T cell È®´ë¹è¾ç ¿¹
[¹æ¹ý]
Peripheral blood mononuclear cells (PBMC)¸¦ Anti-CD3 mAb¿Í RetroNectin¢çÀÌ ¸ðµÎ Æ÷ÇÔµÈ CultiLife¢â 215 Culture bag¿¡¼­ co-stimulationÇϸç 4ÀÏ°£ ¹è¾ç ÈÄ, IL-2¸¦ Æ÷ÇÔÇÑ ¸²ÇÁ±¸ ¹è¾ç¿ë ¹èÁö¿¡¼­ Ç÷û (human AB serum)ÀÇ Æ÷ÇÔ ¶Ç´Â ¹ÌÆ÷ÇÔ Á¶°ÇÀ¸·Î 10ÀÏ°£ ¹è¾çÇÏ¿´´Ù.
Ç÷û ÷°¡ Á¶°ÇÀ¸·Î Day 0~Day 7Àº 1%ÀÇ Ç÷ûÀ» Æ÷ÇÔ, Day 7~Day 10Àº 0.5%ÀÇ Ç÷ûÀ» Æ÷ÇÔÇÏ´Â Á¶°Ç¿¡¼­ ¹è¾çÇÏ¿´´Ù.

[°á°ú]

Ç÷û (Human AB serum)À» ÷°¡ÇÏÁö ¾ÊÀº ¹è¾ç Á¶°Ç¿¡¼­µµ ¸Å¿ì ³ôÀº È®´ë ¹è¾ç·üÀ» º¸¿´°í, È®´ë ¹è¾çµÈ T cell Áß¿¡´Â naive T cellÀÇ ºñÀ²ÀÌ Å¸»ç¹èÁö ´ëºñ ³ôÀº °ÍÀ» È®ÀÎÇÏ¿´´Ù.
º¸Á¸ 2~8¡É
Reference
Takara Bio°¡ °³¹ßÇÑ "RetroNectin¢ç stimulation T cell expansion"´Â ¼Ò·®ÀÇ PBMC¿¡¼­ T cellÀ» È¿À²ÀûÀ¸·Î Áõ½ÄÇÒ ¼ö ÀÖ´Â ¹æ¹ýÀÔ´Ï´Ù. T cell µîÀÇ ÀÓÆı¸¸¦ ÀÌ¿ëÇÏ´Â ¼¼Æ÷ ÀÇ·á, À¯ÀüÀÚ Ä¡·áÀÇ ¼¼Æ÷ °¡°ø ±â¼ú·Î¼­ ÀÓ»ó¿¡ ÀÀ¿ëµÇ°í ÀÖ½À´Ï´Ù.
* º» ¹æ¹ýÀ» ¿¬±¸ ¸ñÀû ÀÌ¿ÜÀÇ ¸ñÀûÀ¸·Î ½Ç½ÃÇÏ´Â °æ¿ì °³º°ÀûÀ¸·Î Takara Bio¿ÍÀÇ ¶óÀ̼±½º °è¾à ü°áÀÌ ÇÊ¿äÇÕ´Ï´Ù.

Keyword :

T cell therapy ¿¬±¸
>
Retrovirus ¹× LentivirusÀÇ transduction enha..
RetroNectin¢ç (Recombinant Human Fibronectin Fragment)
>
T cell stimulation¿ë Ab
Anti-CD3 mAb GMP grade
>
T cell ¹è¾ç¹èÁö (Xeno-Free, Serum-Free)
LymphoONE¢ç T-Cell Expansion Xeno-Free Medium
>
¸é¿ª ¹ÝÀÀ ½ºÅ©¸®´× ¹× ¸ð´ÏÅ͸µÀ» À§ÇÑ
Cytokine GoStix¢â Plus assays
>
¸²ÇÁ±¸ ¹è¾ç¿ë ¹èÁö
GT-T551 Culture medium
>
Primary T cell ¹è¾ç¿¡ ÃÖÀû
¼¼Æ÷ ¹è¾ç¿ë °¡½º Åõ°ú¼º Bag