제품설명
Ingenio® Electroporation Kit는 핵산도입이 어려운 mammalian cell, 특히 primary cell에 대하여 합리적인 비용으로 높은 효율의 핵산도입과 저독성을 실현한 electroporation전용 시약이다. 본 제품은 Electroporation solution, cuvettes, Cell Dropers로 구성되어 있고, Bio-Rad, Lonza, BTX, Eppendolf 등의 electroporation 장비에 최적의 효율을 가지도록 고안되었기 때문에 별도의 전용장비를 구매할 필요가 없다.
Ingenio® 0.2㎝ cuvettes는 Amaxa® Nucleofector® II/2b Device에 적합하고, Ingenio® 0.4 ㎝ cuvettes는 Bio-Rad Gene Pulser Xcell™ Electroporator에 적합하다.
- Ingenio® Pulse Condition Recommendations (pdf)
- Electroporating Primary cells and Other Hard to Transfect Cell Types using Ingenio® Electroporation Solution (pdf)
- Electroporation of Human Induced Pluripotent Stem (iPS) Cells with Ingenio® Electroporation Solution (pdf)
내용
Ingenio® Electroporation Kits with cuvettes
Code |
구성품 |
적용가능한
기기 |
Ingenio® Solution |
Cuvettes |
Cell Droppers |
MIR 50112 |
6.25 ㎖ |
0.2 ㎝cuvettes × 25개 |
25 개 |
- Lonza Amaxa® Nucleofector®
II/2b Device |
MIR 50115 |
12.5 ㎖ |
0.2 ㎝cuvettes × 50개 |
50 개 |
MIR 50118 |
12.5 ㎖×2 |
0.2 ㎝cuvettes × 100개 |
100 개 |
MIR 50113 |
6.25 ㎖ |
0.4 ㎝cuvettes × 25개 |
25 개 |
- Bio-Rad Gene Pulser Xcell™ Electroporator
- Harvard-BTX |
MIR 50116 |
12.5 ㎖ |
0.4 ㎝cuvettes × 50개 |
50 개 |
MIR 50119 |
12.5 ㎖×2 |
0.4 ㎝cuvettes × 100개 |
100 개 |
Ingenio® Electroporation Solution
Code |
용량 |
MIR 50111 |
6.25 ㎖ |
MIR 50114 |
12.5 ㎖ |
MIR 50117 |
12.5 ㎖×2 |
보존
Ingenio® Solution : 4℃
Ingenio® Cuvettes : 실온
Ingenio® Cell Dropers : 실온
실험 예
1. Lonza Amaxa® Nucleofector® II/2b Device에 적용예
Ingenio
® Electroporation Kits were used to transfect indicated cell types using Amaxa
® Nucleofector
® II Device. Cells were assayed at 24 hours by flow cytometry and reported as percentage of live cell population.
*Primary cell type
2. Bio-Rad Gene Pulser Xcell™ Electroporator에 적용예
Cells were electroporated with an EGFP reporter vector using the Ingenio
® Electroporation Solution and a Gene Pulser Xcell™ Eukaryotic System. EGFP expressing cells were identified 24 hours
post-electroporation by flow cytometry and presented as a percentage of the live cell population.
관련제품
- 만능 Transfection reagent: TransIT®-X2 Dynamic Delivery System
- Cas9 protein: Guide-it™ Recombinant Cas9 (Electroporation-Ready)
- 정확도 높은 Cloning용 PCR 효소: PrimeSTAR® GXL DNA Polymerase
