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Home > ÀüÁ¦Ç°º¸±â > DNA/RNA Á¤Á¦ ¡¤ Ç¥½Ä > DNA£¯RNA Ç¥½Ä > Label IT Nucleic Acid Modifying Reagent

Label IT Nucleic Acid Modifying Reagent

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(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 3925
Label IT AMINE Nucleic Acid Labeling Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
Labels 25 ug
(Package)
°¡°Ý¹®ÀÇ   ml012_label_it_nucleic_acid_modifying_kit (1).pdf
Mirus
MIR 3900
Label IT AMINE Nucleic Acid Labeling Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
Labels 100 ug
(Package)
°¡°Ý¹®ÀÇ  

  • DNA³ª RNA ÁÖÇüÀÇ º¯Çü - nucleic acidÀÇ amine ±×·ì¿¡ Á÷Á¢ °øÀ¯ °áÇÕ
  • One-step Chemical Method - nucleic acid modificationÀÇ ³óµµ(¹Ðµµ)¸¦ ÀÏ°üµÇ°í ½±°í Á¶Àý
  • Covalent Mechanism - ¿µ±¸ÀûÀÌ°í ºñÆı«ÀûÀÎ nucleic acid modificationÀ¸·Î proteinÀ̳ª peptide¿Í °áÇÕÇϰųª À¯¸®Ç¥¸éÀ̳ª bead ¶Ç´Â plate¿¡ ºÎÂø °¡´É
Kit Components
Label IT Reagent, Label IT Reconstitution Solution, Labeling Buffer, Solutions and G50 Spin Columns.



Figure 1. The Label IT¢ç Nucleic Acid Modifying Kit. The Label IT nucleic acid modifying reagent is composed of three regions: the reactive NH2 modifying group (green), the linker (yellow) which facilitates electrostatic interactions with nucleic acids and the reactive alkylating group (blue) that covalently attaches the Label IT modifying reagent to any reactive heteroatom within nucleic acids. Attachment of the Label IT modifying reagent to nucleic acids does not alter the structure of the nucleic acids or affect downstream hybridization performance. Once labeled, the nucleic acids can be coupled to reactive substrates of other chemical entities through the added NH2 reactive groups.



Figure 2. Demonstration of the Functionality of Amine Groups on Modified DNA. Plasmid DNA (5.6 kb) was reacted with Label IT Amine Reagent at the ratios (w:w) of labeling reagent to DNA indicated and purified by ethanol precipitation. Five ¥ìg of the modified DNA, in triplicate, was reacted with 10 mM NHS ester TM-Rhodamine and 100 mM NaHCO3 (pH 8 - 8.5) for 1 hour at room temperature in the dark. The DNA was again ethanol precipitated, washed extensively, and assayed for labeling efficiency.

Keyword : MIR3900, MIR3925, v3900,v3925