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Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö

Cell & Sup. Purification Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6676
AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes)
°ü·ÃÇмú ±¸¸ÅÇϱâ
4ȸ
(Package)
1,105,600¿ø 
1,382,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6676-6678_e.v2108Da.pdf
Takara
6677
AAVpro¢ç Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
(Package)
648,000¿ø 
810,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6676-6678_e.v2108Da.pdf
Takara
6678
AAVpro¢ç Purification Pack Maxi (All Serotypes)
°ü·ÃÇмú ±¸¸ÅÇϱâ
4ȸ
(Package)
580,000¿ø 
725,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6676-6678_e.v2108Da.pdf

  • AAV packaging cell°ú ¹è¾ç »óµî¾× ¸ðµÎ¿¡¼­ AAV Á¤Á¦
  • ´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ Àû¿ë °¡´É
  • ³ôÀº ¼öÁØÀÇ AAV recovery¿Í Á¤Á¦ È¿À²
  • µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ÃÊ °£´Ü AAV ÃßÃâ ¹æ½Ä äÅÃ
    : Ultracentrifugation ȤÀº freeze-thaw¿Í °°Àº º¹ÀâÇÑ ¹æ¹ý ºÒÇÊ¿ä
  • All-in-one kit : AAV Á¤Á¦¿¡ ÇÊ¿äÇÑ ¸ðµç ±¸¼º Æ÷ÇÔ
Á¦Ç° ¼³¸í
Adeno-Associated Virus (AAV)´Â ºÐ¿­, ºñºÐ¿­ ¼¼Æ÷¿¡ À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϸç, ƯÈ÷ ºñºÐ¿­ ¼¼Æ÷¿¡¼­´Â Àå±â°£ À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ, ¸é¿ª¿ø¼º (immune response)ÀÌ ³·¾Æ in-vivo ½ÇÇè¿¡ ÃÖÀûÀÌ´Ù. ÀÌ·¯ÇÑ AAV¸¦ ½ÇÇè µ¿¹°¿¡ µµÀÔÇÒ ¶§¿¡´Â, packaging cell°ú ¹è¾ç ¹èÁö·ÎºÎÅÍ À¯·¡ÇÑ ºÒ¼ø¹°µéÀ» Á¦°ÅÇØ¾ß ÇÑ´Ù.

AAV Á¤Á¦¿¡´Â ÀϹÝÀûÀ¸·Î CsCl2/iodixanol gradient ultracentrifugation ¹ýÀ» »ç¿ëÇÏÁö¸¸ °íµµÀÇ ¼÷·ÃµÈ ±â¼úÀ» ÇÊ¿ä·Î Çϸç, ȸ¼öÀ²ÀÌ ³·°í, ½Ã°£ÀÌ ¿À·¡ ¼Ò¿äµÇ´Â ´ÜÁ¡ÀÌ ÀÖ´Ù.

AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes) (Code 6676)Àº T225 flask 5°³ (1ȸ ´ç)¿¡¼­ ¹è¾çÇÑ AAV packaging cell°ú ¹è¾ç »óµî¾×¿¡¼­ ´Ù¾çÇÑ serotypeÀÇ AAV¸¦ ½±°Ô Á¤Á¦ÇÒ ¼ö ÀÖÀ¸¸ç, ¾à 7½Ã°£ÀÌ ¼Ò¿äµÈ´Ù. AAV ÃßÃâ °úÁ¤Àº µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ ¸Å¿ì °£´ÜÇÑ ¹æ½ÄÀ» ÀÌ¿ëÇÏ°í ÀÖÀ¸¸ç, ultracentrifugation ȤÀº freeze-thaw¿Í °°Àº º¹ÀâÇÑ ¹æ¹ýÀ» ÇÊ¿ä·Î ÇÏÁö ¾Ê´Â´Ù
AAV ÃßÃâ ¹× Á¤Á¦ workflow
Á¦Ç° ¼º´É È®ÀÎ
1) AAVÀÇ Á¤Á¦ ¹× ¼øµµ ÃøÁ¤
ZsGreen1 Çü±¤´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â °¢ serotypeÀÇ AAV (AAV1, AAV2, AAV5, AAV6)¸¦ packaging ÇßÀ¸¸ç, AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes) (Code 6676)À» ÀÌ¿ëÇØ ÃÑ 5°³ÀÇ T225 flask¿¡¼­ ¹è¾çµÈ packaging cell°ú ¹è¾ç »óµî¾×À¸·ÎºÎÅÍ AAV¸¦ ÃßÃâ ¹× Á¤Á¦ÇÏ¿´´Ù. ÀÌ·¸°Ô Á¤Á¦ÇÑ AAV¸¦ AAVpro¢ç Titration Kit (for Real Time PCR) (Code 6233)·Î ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿©, 1X109 vg/lane À¸·Î SDS-PAGE¸¦ ¼öÇàÇÏ¿´´Ù.
±× °á°ú, °¢ serotype º° ¸ðµç AAV¿¡¼­ AAV capsid proteinÀÎ VP1, VP2, VP3 band¸¦ È®ÀÎÇÏ¿´´Ù.


2) Á¤Á¦µÈ AAVÀÇ °¨¿° È¿À² ºñ±³
1)¿¡¼­ Á¤Á¦ÇÑ serotype º° AAV¸¦ 293T cell°ú HeLa cell¿¡ °¢°¢ transduction ÇÑ ÈÄ °¨¿° È¿À²À» Æò°¡ÇÏ¿´´Ù. Á¤Á¦ÇÑ AAV¸¦ °¢ ¼¼Æ÷¿¡ 50,000 vg/cell ·Î °¨¿°½ÃŲ 3ÀÏ ÈÄ flow cytometry·Î ºÐ¼®ÇÑ °á°ú, º» Á¦Ç°À» ÀÌ¿ëÇØ Á¤Á¦ÇÑ ÈÄ¿¡µµ °¨¿° ´É·ÂÀ» À¯ÁöÇÏ°í ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.


3) ±âÁ¸ Á¦Ç°°úÀÇ Á¤Á¦ È¿À² ºñ±³
´Ù¾çÇÑ serotypeÀÇ AAV¿¡¼­ AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes) (Code 6676)À» »ç¿ëÇØ Á¤Á¦ÇÑ ¾çÀ» 100%·Î ÇßÀ» ¶§ ±âÁ¸ Á¦Ç°ÀÎ AAVpro¢ç Purification Kit Maxi (All Serotypes) (Code 6666)À¸·Î Á¤Á¦ÇÑ AAVÀÇ ¾çÀ» ºñ±³ÇÏ¿´´Ù. AAV´Â serotypeÀ̳ª À¯ÀüÀÚ¿¡ µû¶ó ¼¼Æ÷ ¹ÛÀÇ ¹èÁö¿¡µµ ¸¹Àº ¾çÀÌ Á¸ÀçÇÏ´Â °æ¿ì°¡ È®ÀεȴÙ. ÀÌ Á¦Ç°À» »ç¿ëÇÏ¸é ¼¼Æ÷ ¹ÛÀÇ ¹èÁö¿¡ Á¸ÀçÇÏ´Â AAV±îÁö ȸ¼öÇØ, ¼¼Æ÷ ¾ÈÀÇ AAV¸¸À» Á¤Á¦ÇÏ´Â Á¦Ç°¿¡ ºñÇØ ´õ¿í °í¼öÀ²·Î AAV¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
³»¿ë
AAVpro¢ç Cell & Sup. Purification Kit Maxi (All Serotypes) (Code 6676)
- AAVpro¢ç Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes) (Code 6677)
- AAVpro¢ç Purification Pack Maxi (All Serotypes) (Code 6678)

AAVpro¢ç Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes) (Code 6677)
- AAV Extraction Solution C

8 §¢

- AAV Extraction Solution D

32 §¢

- AAV Extraction enhancer

80 §¢

- Concentrating Solution*1

115 §¢ X 2

- Dissolving Solution

50 §¢

*1 Concentrating Solution¸¦ 4¡ÆC¿¡¼­ º¸°üÇßÀ» ¶§ ħÀü¹°ÀÌ ¹ß»ýÇÒ ¼ö ÀÖÀ¸³ª, ÀÌ´Â ½Ã¾àÀÇ Ç°ÁúÀ̳ª ¼º´É¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â´Ù. ¸¸ÀÏ Ä§Àü¹°ÀÌ ¹ß»ýÇÑ °æ¿ì, 40¡ÆC¿¡¼­ ¿ÏÀüÈ÷ ³ìÀÎ ÈÄ »ç¿ëÇÑ´Ù.

AAVpro¢ç Purification Pack Maxi (All Serotypes) (Code 6678)
- Precipitator A*2

5 §¢

- Precipitator B

2.5 §¢

- Millex-HV 0.45 §­

4 pcs

- Amicon Ultra-15, 100 kDa

4 pcs

- Suspension Buffer

60 §¢ X 2

- Cryonase Cold-active Nuclease

500 §¡

*2 Precipitator A¸¦ ³·Àº ¿Âµµ¿¡¼­ º¸°üÇßÀ» ¶§ ħÀü¹°ÀÌ ¹ß»ýÇÒ ¼ö ÀÖÀ¸³ª, ÀÌ´Â ½Ã¾àÀÇ Ç°ÁúÀ̳ª ¼º´É¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â´Ù. ¸¸ÀÏ Ä§Àü¹°ÀÌ ¹ß»ýÇÑ °æ¿ì, 37¡ÆC¿¡¼­ ¿ÏÀüÈ÷ ³ìÀÎ ÈÄ »ç¿ëÇÑ´Ù.
°ü·ÃÁ¦Ç°

Keyword : AAV,Adeno-associated virus,extract,extraction,ÃßÃâ,purification,Á¤Á¦,concentration,³óÃà,transduction,°¨¿°,in-vivo,in vivo,invivo,Àκñº¸,µ¿¹°½ÇÇè,½ÇÇ赿¹°,¸é¿ª¿ø¼º

Adeno-Associated Virus (AAV)
>
Adeno-associated virus Á¦ÀÛ¿¡ ÃÖÀûÀÎ packagi..
Packaging (293T Cell Line)
>
Helper Free ½Ã½ºÅÛ¿¡ ÀÇÇÑ ´Ù¾çÇÑ serotypeÀÇ ..
AAV1, 2, 5, 6 Vector System
>
Cre-Recombinase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2, ..
CRE Recombinase System
>
¥â-galactosidase À¯ÀüÀÚ¸¦ žÀçÇÑ AAV1, AAV2,..
LacZ System
>
Çü±¤´Ü¹éÁúÀÌ Æ÷ÇÔµÈ AAV vector
pAAV-ZsGreen1 Vector
>
Adeno-associated virus delivery-CRISRP/Cas9 ..
CRISPR/Cas9 Vector System
>
SaCas9¸¦ ÀÌ¿ëÇÑ AAV mediated CRISPR Genome E..
CRISPR/SaCas9 Vector System (AAV2)
>
AAV2 Delivery Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Inducible System (AAV2)
>
Cell°ú ¹è¾ç ¹èÁö¿¡¼­ AAV ÃßÃâºÎÅÍ Á¤Á¦±îÁö
Cell & Sup. Purification Kit
>
´Ù¾çÇÑ serotypeÀÇ AAV Á¤Á¦¿¡ ÃÖÀû
Purification Kit Maxi/Midi (All Serotypes)
>
AAV Serotype 2 Á¤Á¦ kit
Purification Kit (AAV2 Àü¿ë)
>
AAV¸¦ È¿À²ÀûÀ¸·Î ³óÃà
AAVpro¢ç Concentrator
>
ºü¸£°í Á¤È®ÇÑ AAV ¿ª°¡ÃøÁ¤ (qPCR)
AAVpro¢ç Titration Kit (for Real Time PCR)
>
°£ÆíÇÑ AAV ÃßÃâ½Ã¾à
Extraction Solution
>
±âÁ¸ÀÇ AAV ÃßÃâ ¹æ¹ýÀ¸·Î Á¤Á¦±îÁö 4½Ã°£ ³» ..
AAV Freeze-thaw extraction solution