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Home > 전제품보기 > Gene transfer (virus) > Recombinant Lentivirus 관련 > Lenti-X™ Packaging single shots

Lenti-X™ Packaging single shots

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비고 사용자매뉴얼
Clontech
631275
Lenti-X™ Packaging Single Shots (VSV-G)
관련학술 관심상품등록 구매하기 라이선스 
16 회
1,456,000원  제조사 페이지로 바로가기 119724
Clontech
631276
Lenti-X™ Packaging Single Shots (VSV-G)
관련학술 관심상품등록 구매하기 라이선스 
96 회
3,446,000원  제조사 페이지로 바로가기
Clontech
631277
Lenti-X™ Packaging Single Shots (Integrase Deficient)
관련학술 관심상품등록 구매하기 라이선스 
16 회
1,441,000원  제조사 페이지로 바로가기 103226
Clontech
631278
Lenti-X™ Packaging Single Shots (Ecotropic)
관련학술 관심상품등록 구매하기 라이선스 
16 회
1,441,000원  제조사 페이지로 바로가기 103226

* Lenti-X™ Packaging Single shots은 기존의 Lenti-X™ HTX Packaging System의 업그레이드 제품입니다.
  • convenience with high performance
  • Consistently high titers (107-108 IFU/ml)
  • Simple protocol-Just add your lentiviral DNA, mix, and add to 293T cells
  • No optimization or additional transfection reagent required

Lenti-X™ Packaging Single Shots (VSV-G)는 간단하고 효율적으로 고역가의 렌티바이러스를 제작할 수 있는 Clontech의 4세대 Lentiviral packaging system이다. Lenti-X™ Packaging Single Shots (VSV-G)는 VSV-G 타입의 pseudotype Lenti-X™ Lentivral packaging plasmid에 최적화되어있는 Xfect™ Transfection reagent가 미리 포함된, pre-aliquoted, lyophilized 시약이기 때문에 실험 과정 중에서 trasnfection reagent를 따로 사용하지 않아도 되는 편리한 제품이다.



그림 1. The Lenti-X™ Packaging Single Shots (VSV-G) protocol.



그림 2. Lenti-X™ Packaging Single Shots (VSV-G) consist of single tubes of lyophilized Xfect™ Transfection Reagent premixed with VSV-G pseudotyped lentiviral packaging plasmids. To produce high-titer lentivirus, simply add your lentiviral vector in sterile water to a tube, mix, and add to 293T cells.



그림 3. Consistent, high-efficiency transfections lead to high titers. A lentiviral vector containing the ZsGreen1 gene was packaged according to the Lenti-X™ Single Shots protocol in four independent experiments. Briefly, 7 μg of pLVX-ZsGreen1 plasmid was added to each of four Lenti-X™ Single Shots; the tubes were vortexed for 20 sec and incubated at room temperature for 10 min. Then, the mixture was added to cultured Lenti-X™ 293T cells that were approximately 80% confluent. Forty-eighty hours after transfection, the cells were imaged by fluorescence microscopy (Panel A, top) and light microscopy (Panel A, bottom). After images were taken, the supernatant was harvested and used infect HT1080 cells for titer determination (Panel B, IFU/ml).
적용
- Lentiviral transduction of a broad range of hard-to-transfect cell types
- Easily create stable cell lines
- Create VSV-G pseudotyped lentivirus
구성품 (자세한 내용은 CoA를 참조하세요)
* Lenti-X™ Packaging Single Shots (VSV-G) (Code 631275)
   1 box containing 16 Lenti-X™ Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X™ Packaging Single Shots (VSV-G) (Code 631276)
   1 box containing 96 Lenti-X™ Packaging Single Shots (VSV-G), in a foil pouch

* Lenti-X™ Packaging Single Shots (Ecotropic) (Code 631277)
   1 box containing 16 Lenti-X Packaging Single Shots (Ecotropic), in a foil pouch

* Lenti-X™ Packaging Single Shots (Integrase deficient) (Code 631278)
   1 box containing 16 Lenti-X™ Packaging Single Shots (Integrase Deficient), in a foil pouch

Keyword : Lentivirus,Lenti-virus,packaging,Lentiviral,Lentivral delivery,Lenti
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Recombinant Lentivirus 관련
> Clontech Lentiviral Product 선택 가이드
> [Overview] Lenti-X Packaging Single Shots
> RetroNectin® – Viral transduction, T-cell expansion 적용례
> Lenti-X Lentivirus 유전자 발현 시스템
> Lenti-X™ Packaging single shots
> Lentivirus Tet-Inducible expression 시스템
Single vector의 Lenti-viral Tet 유도발현 시스템
> Lenti-X Tet One Inducible Expression System
> Lenti-X Tet-On 3G Inducible Expression System
> Lentiviral Vector Systems with an EF-1 Alpha Promo
> Lenti-X Repoter 시스템
> Lentiviral Vectors for Fluorescent Protein Express
> 신속한 Lentivirus Titer 측정 키트
> Lenti-X 293T Cell Lines
> Lenti-X Lentivirus 농축 시약
> Lenti-X GoStix (간이 Lentivirus 역가 측정 시약)
> Ecotropic Receptor Booster
> Lenti-X Maxi Purification Kit
> Lenti-X Accelerator Starter Kit
> Lenti-X Integration Site Analysis Kit
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