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Transfection ½Ã¾à
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µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
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Trans
IT¢ç-Oligo Transfection Reagent
Trans
IT¢ç-Oligo Transfection Reagent
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Mirus
MIR 2164
Trans
IT-Oligo Transfection Reagent
0.4 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 2160
Trans
IT-Oligo Transfection Reagent
1 ml
°¡°Ý¹®ÀÇ
Mirus
MIR 2165
Trans
IT-Oligo Transfection Reagent
1 ml¡¿5
°¡°Ý¹®ÀÇ
Mirus
MIR 2166
Trans
IT-Oligo Transfection Reagent
1 ml¡¿10
°¡°Ý¹®ÀÇ
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
Unique Formulation
- Maximize transfection performance of oligonucleotides into a wide range of cells.
Low Cellular Toxicity
- Maintain cell density and reduce experimental biases.
High Efficiency Delivery
- Achieve high transfection efficiency in cells to ensure experimental success.
Figure 1. The TransIT-Oligo Reagent Achieves High Transfection Efficiency.
HeLa cells transfected using TransIT-Oligo Reagent and Label IT Cy™3 and Label IT Fluorescein labeled phosphothioate DNA oligos in complete media for 24 hours.
Figure 2. The
Trans
IT-Oligo Reagent Effectively Transfects a 2'OMe RNA Oligo that Blocks a Cryptic Splice Site.
The HeLa-Luc 705 reporter cell line (Kang et al. 1998, 37:6235) used in this study contains a luciferase reporter construct that has the ©¬-globin 705 intron inserted into the luciferase ORF. A mutation present at position 705 of the ©¬-globin intron activates two cryptic splice sites within the intron that lead to the production of a spliced luciferase mRNA that is disrupted by a small intron with an in-frame stop codon, thus preventing translation of functional luciferase protein. The transfection of a 2'OMe oligonucleotide complementary to the cryptic 705 splice site inhibits splicing at the cryptic splice sites enabling the complete removal of the ©¬-globin intron and production of a mRNA with a complete, uninterrupted luciferase ORF.
The HeLa-Luc 705 cell line was transfected with increasing amounts of the anti-705 splice site 2'OMe RNA oligo at the indicated final concentrations using the
Trans
IT-Oligo Transfection Reagent. The cells were harvested 24 hours post-transfection and assayed for luciferase activity. The increase in luciferase activity indicates effective delivery of the anti-705 splice site RNA oligo using the
Trans
IT-Oligo Reagent.
Keyword : V2160,MIR2160,V2164,MIR2164,V2165T,MIR2165,V2166,MIR2166
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
Large RNA & CRISPR guide RNA¿ëtransfection ..
Trans
IT¢ç-mRNA Transfection Kit
Trans
IT¢ç-Oligo Transfection Reagent
mRNA, sgRNA, siRNAµî ´Ù¾çÇÑ RNA transfection..
Xfect¢â RNA Transfection Reagent
Xfect¢â Protein Transfection Reagent