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Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû

TransIT¢ç-Lenti Transfection Reagent

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 6600
TransIT¢ç-Lenti Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1.5 §¢
°¡°Ý¹®ÀÇ   transit-lenti-reagent-protocol.pdf
Mirus
MIR 6603
TransIT¢ç-Lenti Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.3 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6604
TransIT¢ç-Lenti Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.75 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6605
TransIT¢ç-Lenti Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 x 1.5 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6606
TransIT¢ç-Lenti Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 x 1.5 §¢
°¡°Ý¹®ÀÇ  
Mirus
MIR 6620
TransduceIT¢â Transduction Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 §¢
°¡°Ý¹®ÀÇ   transduceit-reagent-pds.pdf



  • High Performance - Provide up to eight-fold higher functional titers
  • Simple Protocol - No media change required, single harvest
  • Animal Origin Free - Regulatory friendly
Á¦Ç°¼³¸í
TransIT¢ç-Lenti Transfection Reagent´Â adherent HEK 293T ¼¼Æ÷¿¡ packaging vector¿Í lentiviral vector¸¦ È¿À²ÀûÀ¸·Î co-transfectionÇÏ¿© recombinant lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ´Â transfection ½Ã¾àÀÌ´Ù.
TransduceIT¢â Transduction Reagent´Â hexadimethrine bromide ¼ö¿ë¾×À¸·Î retroviral/lentiviral transduction È¿À²À» ³ôÀÌ°íÀÚ ÇÒ ¶§ »ç¿ëÇÑ´Ù.
º¸Á¸ - 20 ¡É
Àû¿ë¿¹
1. High Functional Titers with TransIT¢ç-Lenti Transfection Reagent.


Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP¢â transfer vector and the Lentivirus Packaging Mix powered by MISSION¢ç (1:1 ratio, 2 ¥ìg/well) with the following reagents: TransIT¢ç-Lenti (3:1, vol:wt), Lipofectamine¢â 2000 (3:1), Lipofectamine¢â 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 ¥ìg pDNA/well). The supernatant was harvested, filtered (0.45 ¥ìm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 ¥ìg/ml TransduceIT¢â and GFP expression was measured 72 hours post-transduction using guava easyCyte¢â 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

2. High Transduction Efficiency with Unconcentrated Lentivirus Using TransIT¢ç-Lenti.

(A) Lentivirus was produced with the TransIT¢ç-Lenti Transfection Reagent (3:1, vol:wt) or Lipofectamine¢â 2000 using the MISSION¢ç vectors (pLKO.1-puro-CMV-TurboGFP¢â transfer vector and the Lentivirus Packaging Mix powered by MISSION¢ç). The supernatant was harvested, filtered (0.45 ¥ìm), and frozen. Lentivirus transductions were performed 5 days post-plating with iCell¢ç Motor Neurons (Cellular Dynamics International). For bothTransIT-Lenti and Lipofectamine¢â 2000, one microliter of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency was measured 72 hours post-transduction using guava easyCyte¢â 5HT Flow Cytometer. Error bars represent the SEM of duplicate wells. (B) iCell¢ç Motor Neurons were plated in Ibidy 35mm dishes and transduced withlentivirus produced using the TransIT¢ç-Lenti Transfection Reagent and MISSION¢ç vectors. Images were captured at 72 hours post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.

Keyword :

È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
>
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
TransIT-VirusGEN¢ç Transfection Reagent
>
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
TransIT¢ç-Lenti Transfection Reagent
>
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
>
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
>
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit