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Transfection ½Ã¾à
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È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
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Trans
IT¢ç-Lenti Transfection Reagent
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
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IT¢ç-Lenti Transfection Reagent
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(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 6600
Trans
IT¢ç-Lenti Transfection Reagent
1.5 §¢
°¡°Ý¹®ÀÇ
Mirus
MIR 6603
Trans
IT¢ç-Lenti Transfection Reagent
0.3 §¢
°¡°Ý¹®ÀÇ
Mirus
MIR 6604
Trans
IT¢ç-Lenti Transfection Reagent
0.75 §¢
°¡°Ý¹®ÀÇ
Mirus
MIR 6605
Trans
IT¢ç-Lenti Transfection Reagent
5 x 1.5 §¢
°¡°Ý¹®ÀÇ
Mirus
MIR 6606
Trans
IT¢ç-Lenti Transfection Reagent
10 x 1.5 §¢
°¡°Ý¹®ÀÇ
Mirus
MIR 6620
TransduceIT¢â Transduction Reagent
1 §¢
°¡°Ý¹®ÀÇ
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
High Performance
- Provide up to eight-fold higher functional titers
Simple Protocol
- No media change required, single harvest
Animal Origin Free -
Regulatory friendly
Á¦Ç°¼³¸í
Trans
IT¢ç-Lenti Transfection Reagent´Â adherent HEK 293T ¼¼Æ÷¿¡ packaging vector¿Í lentiviral vector¸¦ È¿À²ÀûÀ¸·Î co-transfectionÇÏ¿© recombinant lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ´Â transfection ½Ã¾àÀÌ´Ù.
Transduce
IT¢â Transduction Reagent´Â hexadimethrine bromide ¼ö¿ë¾×À¸·Î retroviral/lentiviral transduction È¿À²À» ³ôÀÌ°íÀÚ ÇÒ ¶§ »ç¿ëÇÑ´Ù.
º¸Á¸ - 20 ¡É
Àû¿ë¿¹
1. High Functional Titers with TransIT¢ç-Lenti Transfection Reagent.
Adherent 293T/17 cells were transfected in a 6-well plate with pLKO.1-puro-CMV-TurboGFP¢â transfer vector and the Lentivirus Packaging Mix powered by MISSION¢ç (1:1 ratio, 2 ¥ìg/well) with the following reagents:
Trans
IT¢ç-Lenti (3:1, vol:wt), Lipofectamine¢â 2000 (3:1), Lipofectamine¢â 3000 (3:1:1), 25 kDa PEI (6:1), or CaPO4 precipitation (4 ¥ìg pDNA/well). The supernatant was harvested, filtered (0.45 ¥ìm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 ¥ìg/ml
Transduce
IT¢â and GFP expression was measured 72 hours post-transduction using guava easyCyte¢â 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.
2. High Transduction Efficiency with Unconcentrated Lentivirus Using
Trans
IT¢ç-Lenti.
(A) Lentivirus was produced with the
Trans
IT¢ç-Lenti Transfection Reagent (3:1, vol:wt) or Lipofectamine¢â 2000 using the MISSION¢ç vectors (pLKO.1-puro-CMV-TurboGFP¢â transfer vector and the Lentivirus Packaging Mix powered by MISSION¢ç). The supernatant was harvested, filtered (0.45 ¥ìm), and frozen. Lentivirus transductions were performed 5 days post-plating with iCell¢ç Motor Neurons (Cellular Dynamics International). For both
Trans
IT-Lenti and Lipofectamine¢â 2000, one microliter of unconcentrated supernatant was added per well of a 96-well plate. GFP efficiency was measured 72 hours post-transduction using guava easyCyte¢â 5HT Flow Cytometer. Error bars represent the SEM of duplicate wells. (B) iCell¢ç Motor Neurons were plated in Ibidy 35mm dishes and transduced withlentivirus produced using the
Trans
IT¢ç-Lenti Transfection Reagent and MISSION¢ç vectors. Images were captured at 72 hours post-transduction with a Zeiss Axiovert S100 inverted fluorescence microscope using a 63X objective under oil.
Keyword :
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
Trans
IT-VirusGEN¢ç Transfection Reagent
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
Trans
IT¢ç-Lenti Transfection Reagent
Trans
IT-PRO¢ç Transfection Reagent & Kit
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit