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ÆĶóÇÉ ÀýÆíÀ¸·Î ºÎÅÍ DNA ÃßÃâ
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TaKaRa DEXPAT¢â
Dr. GenTLE¢â (from Yea..
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Label-ON Reagents
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TaKaRa DEXPAT™ ´Â FFPE (Formalin fixed paraffin embedded tissue) »ùÇÿ¡¼ PCR¿ë templateÀ¸·Î »ç¿ë °¡´ÉÇÑ DNA¸¦ 1 stepÀ¸·Î ÃßÃâÇÒ ¼ö ÀÖ´Â DNA ÃßÃ⠽þàÀÌ´Ù. ±âÁ¸ÀÇ FFPE »ùÇà Á¤Á¦ ¹æ¹ýÀº ½Ã°£ÀÌ ¿À·¡ °É¸®°í ½ÇÇè °úÁ¤ÀÌ º¹ÀâÇßÁö¸¸ ÀÌ Á¦Ç°À» »ç¿ëÇÏ¸é ½Å¼ÓÇÏ°í °£ÆíÇÏ°Ô DNA¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù (±×¸² 1.).ÀÌ Á¦Ç°ÀÇ »ç¿ë½Ã ÀåÁ¡Àº ¾Æ·¡¿Í °°´Ù.
- Å» ÆĶóÇÉ °úÁ¤ »ý·«
- 25ºÐ¸¸¿¡ ÃßÃâ ¿Ï·á
- PCR¿ë templateÀ¸·Î »ç¿ë °¡´ÉÇÑ DNA ÃßÃâ °¡´É (400 bp ÀÌÇÏÀÇ Å¸°Ù ÁõÆø¿¡ »ç¿ë °¡´É)
- µ¶¼º¹°Áú ¹Ì»ç¿ë
- Site-specific extraction¹ýÀ¸·Î ºÐ¼® Á¤È®µµ Áõ°¡
[±×¸² 1. DEXPAT°ú Á¾·¡ÀÇ FFPE »ùÇà Á¤Á¦ ¹æ¹ý ºñ±³]
ÀÌ Á¦Ç°Àº Ư¼ö °è¸éÈ°¼ºÁ¦¿Í PCR ÀúÇع°Áú ÈíÂø ¼öÁö°¡ ÃÖÀû ³óµµ·Î È¥ÇյǾî ÀÖ´Ù. FFPE »ùÇÿ¡ ÀÌ Á¦Ç°À» ÷°¡ÇÏ¿© 100¡É¿¡¼ 10ºÐ°£ °¡¿ ÈÄ 4¡É¿¡¼ ¿ø½ÉºÐ¸® ÇÏ´Â °ÍÀ¸·Î PCR¿ë template DNA¸¦ Á¶Á¦ÇÒ ¼ö ÀÖ´Ù.
´Ü, FFPE »ùÇÿ¡¼ ÃßÃâµÈ DNA´Â »ùÇÃÀÇ FFPE Á¦ÀÛÀ» À§ÇÑ fixation ¹× paraffin embedding °úÁ¤¿¡¼ ºÐÇصǾúÀ» °¡´É¼ºÀÌ ³ôÀ¸¹Ç·Î, ´ëºÎºÐ 400 bp ¹Ì¸¸ÀÇ PCR ÁõÆø¸¸ÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ ÃßÃâµÈ DNAÀÇ quality´Â FFPE »ùÇÃÀÇ »óÅ¿¡ Å« ¿µÇâÀ» ¹Þ´Â´Ù.
[ÁÖÀÇ] ÀÌ Á¦Ç°Àº Æ÷À¯·ù Á¶Á÷ÀÇ FFPE »ùÇÿ¡¼ DNA¸¦ ÃßÃâÇϱâ À§ÇÑ Á¦Ç°À¸·Î, ¼¼±Õ ¹× Áø±ÕÀÇ DNA ÃßÃâ¿¡´Â »ç¿ëÇÒ ¼ö ¾ø´Ù.
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TaKaRa DEXPAT Easy
10 §¢ x 5 °³
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- »ç¿ëÀÌ ´õ¿í Æí¸®ÇØÁø FFPE »ùÇà DNA ÃßÃ⠽þà
TaKaRa DEXPAT™ Easy (Code 9104)
- Spin columnÀ» »ç¿ëÇÑ FFPE »ùÇà DNA ÃßÃâ
TaKaRa MiniBEST FFPE DNA Extraction Kit (Code 9782A)
- PCR inhibitor¿¡ ÀúÇ×¼ºÀÌ ¶Ù¾î³ È¿¼Ò
MightyAmp™ DNA Polymerase Ver.3 (Code R076A)
- PCRÀÇ Á¤È®µµ°¡ Áß¿äÇÑ ½ÇÇè¿¡¼´Â
PrimeSTAR
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GXL Premix (Code R051A)
- Àü±â¿µµ¿½Ã ¹ü¿ëÀûÀ¸·Î »ç¿ë °¡´ÉÇÑ No.1 agarose
SeaKem® LE Agarose (Code 50004)
Keyword :
Genomic DNA ÃßÃâ ¡¤ Á¤Á¦
Universal Genomic DNA Extraction Kit
Plant Genomic DNA Extraction Kit
FFPE »ùÇ÷κÎÅÍ genomic DNA¸¦ ÃßÃâÇÏ´Â
FFPE DNA Extraction Kit
Bacteria Genomic DNA Extraction Kit Ver.3.0
´Ù¾çÇÑ »ùÇÿ¡ »ç¿ëÇÏ´Â DNA °£ÀÌ ÃßÃâ½Ã¾à
MightyPrep reagent for DNA
½Ä¹° genomic DNA °£ÀÌÃßÃ⠽þà
Plant DNA Isolation Reagent
ÆĶóÇÉÀýÆí¿ë 1 step DNA °£ÀÌÃßÃ⠽þà (tube ..
TaKaRa DEXPAT¢â Easy
ÆĶóÇÉ ÀýÆíÀ¸·Î ºÎÅÍ DNA ÃßÃâ
TaKaRa DEXPAT¢â
È¿¸ð·Î ºÎÅÍ °í¼øµµÀÇ Genomic DNA ÃßÃâ
Dr. GenTLE¢â (from Yeast) High Recovery