TaKaRa
로그인  |   회원가입  |   ID/PW 찾기  
제품명/제품코드
사이트내검색
Home > 전제품보기 > 단백질 발현 · 정제 > His-tag 융합 단백질 정제 > Capturem His-Tagged Purification Miniprep Kit
Spin column으로 간편하게 His-tag 단백질 정제

Capturem His-Tagged Purification Miniprep Kit

-

제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
635710
Capturem™ His-Tagged Purification Miniprep Kit
관련학술 관심상품등록 구매하기 라이선스 
20 rxns
277,600원 
347,000원
가격할인
04.17 ~ 05.31
제조사 페이지로 바로가기
103486

Tip. 더욱 효율적인 단백질 추출을 위하여 xTractor Buffer 매뉴얼 (클릭)의 "Lysis protocol"을 참조하세요!
Tip. Maxi-scale (2.5mg/column)의 제품은 Capturem™ His-Tagged Purification Maxiprep Kit (Code 635713)을 참고하세요.

  • No-waiting workflow - 실온에서 5분이면 His-tag 단백질 정제 가능
  • High purity and yield - 1개의 column당 100ug, 농도 ~ 0.3 - 1 mg/ml의 정제 가능
  • Wide range of conditions - Native and denaturing conditions; with common additives (e.g., EDTA, DTT, βME, glycerol, TCEP, etc.)
  • Lysis buffer compatibility - xTractor Buffer 등 다양한 Lysis buffer 사용 가능
  • Different cell systems - Mammalian cell, bacterial cell 등 다양한 샘플 적용 가능
제품설명
Next-generation membrane technology makes the His-Tagged Purification Miniprep Kit a revolutionary solution for recombinant protein purification, capable of outperforming conventional methods under a wide range of conditions. Where resin columns fall short, this new system provides high capacity and purity in just five minutes, from loading sample application through elution. Novel nylon membranes have been assembled into spin columns, creating a no-waiting, room-temperature workflow compatible with both mammalian and bacterial cells. Exceptional performance is consistent under a wide range of research settings, including denaturing conditions, in the presence of additives (e.g., βME, EDTA, DTT, glycerol, TCEP, etc.; see table below), and when performing tandem purification of his-tagged proteins.



그림 1. Protein purification miniprep workflow. Each mini spin column can be loaded with up to 800 μl of lysate (yielded from 2?5 ml of culture). His-tagged protein is first bound to the membrane, followed by washing with 300 μl wash buffer, and elution with 300 μl elution buffer. Over 90% of the bound protein can be eluted with as little as 100 μl elution buffer. Each step is followed by spinning the tube for 1 min at 11,000 x g. The working bed volume of the membrane is <2 μl. This entire purification is complete in <5 min.



그림 2. Purification of GFPuv under native and denaturing conditions. Spin columns were loaded with 800 μl of cell lysate, and all steps were performed with appropriate buffers for native and denaturing conditions. 8 M urea or 6 M guanidine were included in appropriate samples.
Reagents Compatible with His-Tagged Purification Miniprep Kits

Reagent

Compatibility*

MOPS

200 mM

HEPES

200 mM

Tris

200 mM

EDTA

10 mM

Beta-mercaptoethanol

30 mM

DTT

10 mM

TCEP

5 mM

Guanidine-HCl

6 M

Urea

8 M

Nonionic detergent (Triton X-100)

2%

Nonionic detergent (Tween 20)

2%

Anionic detergent (SDS)

1%

Arginine

500 mM

Glycine

100 mM

Histidine

20 mM

Sodium chloride

2 M

Imidazole

40 mM

Glycerol

10%

적용
  • Biochemical/enzymatic assays
  • Pull-down or other protein interaction assays
  • Confirmation screening
  • Protein/ligand affinity analysis
  • Crystallography or other structural analyses
  • Antibody production
  • Toxicology studies
  • Protein transfer to live cells
구성품 (자세한 내용은 CoA를 참조하세요)
  • 20 ea Mini Spin Columns
  • 2 x 15 ml xTractor Buffer
  • 10 ml Wash Buffer
  • 10 ml Elution Buffer

Keyword :
-
His-tag 융합 단백질 정제
> His-tag 융합 단백질 정제제품 선택 가이드
> His-tag purification Overview
차세대 His-tag purification system
> 차세대 단백질 정제, Capturem™
Spin column으로 간편하게 His-tag 단백질 정제
> Capturem His-Tagged Purification Miniprep Kit
실온에서 15분이면 고용량의 His-tag 단백질 정제가..
> Capturem™ His-Tagged Purification Maxiprep Kit
96well plate type으로 여러 샘플을 한꺼번에 정제
> Capturem His-Tagged Purification 96
Histidine tag 융합 단백질의 고순도 정제에
> TALON Resin
> TALON Resin(Magnetic Bead)
TALON Resin 충진한 Pre-packed type
> HisTALON Cartridges & Gravity Columns
대장균 배양액으로부터 One-step으로 His-tag 융합 ..
> TALON Single Step Columns
대장균으로부터 His-tag 융합 단백질 추출에,xTract..
> TALON xTractor Buffer Kit
Histidine tag 융합 단백질의 고수율로 정제
> His60 Ni Superflow Resin & Gravity Columns
His60 Ni resin 충진제 Pre-packed typ
> His60 Ni Superflow Cartridges & Gravity Columns
> His60 Ni Magnetic Beads
4℃에서 활성을 띄는 HN-tag를 갖고 있는 단백질 분..
> HRV 3C Protease
-