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¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ

Transfer Vectors

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
AB Vector
AV200
pVL1393
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV201
pAcAB3
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV202
pAB-bee
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV203
pAB-bee-8xHis
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV204
pAB-bee-FH
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV205
pAB-6xHis
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV206
pAB-GST
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV207
pAB-MBP
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV208
pAB-6xHis-MBP¢â
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV209
pVL-GFP
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  
AB Vector
AV210
pVL-FH
°ü·ÃÇмú ±¸¸ÅÇϱâ
25 ¥ìg
°¡°Ý¹®ÀÇ  

Á¦Ç°¼³¸í
º» transfer vector ½Ã¸®Áî´Â ¸ñÀû À¯ÀüÀÚ¸¦ Ŭ·Î´× ÇÑ ÈÄ, ProFold¢â baculovirus DNA¿Í ÇÔ²² °ïÃæ ¼¼Æ÷ÁÖ (Sf9, Sf21µî)¿¡ co-transfection Çϸé polyhedrin ºÎÀ§ÀÇ homologous recombinationÀ» ÅëÇØ ¸ñÀûÀ¯ÀüÀÚ¸¦ ¹ßÇöÇÏ´Â recombinant baculovirus¸¦ È¿À²ÀûÀ¸·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
¸ñÀû À¯ÀüÀÚ´Â recombinant baculovirusÀÇ °ïÃæ¼¼Æ÷ °¨¿° ¸»±â¿¡ ¸ñÀû´Ü¹éÁúÀÇ ´ë·® ¹ßÇöÀ» À¯µµÇÏ´Â °­·ÂÇÑ polyhedrin promoter ¶Ç´Â p10 promoterÀÇ Á¦¾î¸¦ ¹Þ´Â´Ù. µ¿½Ã¿¡ ¹ßÇö½Ãų ¸ñÀû À¯ÀüÀÚÀÇ ¼ö, ER ¼ö¼Û ¿©ºÎ, fusion tag¿¡ µû¶ó ´Ù¾çÇÑ transfer vector¸¦ ¼±ÅÃÇÒ ¼ö ÀÖ´Ù.

Ç¥1. °¢ vectorÀÇ Æ¯Â¡

Á¦Ç°¸í

Code

Vector size

Ư¡

pVL1393

AV200

9,639 bp

ÀϹÝÀûÀÎ transfer vector
- Polyhedrin promoterÀÇ Á¦¾î ÇÏ¿¡ ¸ñÀû À¯ÀüÀÚ ¹ßÇö

pAcAB3

AV201

10,096 bp

Subunit ±¸¼º ´Ü¹éÁú¿¡ ÃÖÀû; ¸ñÀû ´Ü¹éÁúÀ» 3°³±îÁö µ¿½Ã ¹ßÇö °¡´É
- Polyhedrin promoterÀÇ Á¦¾î ÇÏ¿¡ 1°³, 2°³ÀÇ p10 promoter Á¦¾î ÇÏ¿¡ °¢°¢ 1°³¾¿, ÃÖ´ë 3°¡Áö ¸ñÀû À¯ÀüÀÚ ¹ßÇö °¡´É

pAB-bee¢â

AV202

9,932 bp

ER ¼ö¼Û¼º ºÐºñ ´Ü¹éÁú, ¸·´Ü¹éÁú ¹ßÇö¿¡ ÃÖÀû
- Polyhedrin promoter
- Honeybee melitin signal sequence, MSC, 8 x His tag ·Î±¸¼º
- ¸ñÀû À¯ÀüÀÚÀÇ N ¸»´Ü¿¡ melitin signal sequence ºÎ°¡ÇÏ¿© °ïÃæ¼¼Æ÷³»¿¡¼­ ER·Î È¿À²ÀûÀÎ ¼ö¼Û °¡´É

pAB-bee¢â-8xHis

AV203

9,965 bp

ER ¼ö¼Û¼º ºÐºñ ´Ü¹éÁú, ¸·´Ü¹éÁú ¹ßÇö¿¡ ÃÖÀû
- Polyhedrin promoter
- Honeybee melitin signal sequence, 8 x His tag, MSC, 8 x His tag ·Î ±¸¼º

pAB-bee¢â-FH

AV204

9,783 bp

ER ¼ö¼Û¼º ºÐºñ ´Ü¹éÁú, ¸·´Ü¹éÁú ¹ßÇö¿¡ ÃÖÀû
- Polyhedrin promoter
- Honeybee melitin signal sequence, MSC, FLAG tag, 8 x His tag ·Î ±¸¼º

pAB-6xHis¢â

AV205

9,698 bp

- Polyhedrin promoter
- 6 x His tag, Thrombin Àý´ÜºÎÀ§, MSC ·Î ±¸¼º

pAB-GST¢â

AV206

10,355 bp

- Polyhedrin promoter
- GST tag, MSC ·Î ±¸¼º

pAB-MBP¢â

AV207

10,793 bp

- Polyhedrin promoter
- MBP tag, Thrombin Àý´ÜºÎÀ§°¡ Æ÷ÇÔµÈ MSC ·Î ±¸¼º

pAB-6xHis-MBP¢â

AV208

10,833 bp

- Polyhedrin promoter
- 6 x His tag, MBP tag, Thrombin Àý´ÜºÎÀ§°¡ Æ÷ÇÔµÈ MSC ·Î ±¸¼º

pVL-GFP

AV209

10,371bp

- Polyhedrin promoter
- MSC, GFP Çü±¤´Ü¹éÁú ¼­¿­·Î ±¸¼º

pVL-FH

AV210

9,723 bp

- Polyhedrin promoter
- MCS, FLAG tag, 8 x His tag ·Î ±¸¼º

³»¿ë °¢ transfer vector 50 §¡ (25 §¶)
º¸Á¸ -20 ¡É
»ç¿ë»ó ÁÖÀÇ
Á¦Ç° ³»¿¡ »ç¿ë ¸Å´º¾óÀÌ Æ÷ÇԵǾî ÀÖÁö ¾Ê½À´Ï´Ù. Homologous recombination¿¡ µû¸¥ ÀϹÝÀûÀÎ recombinant baculovirus ±¸Ãà ¹æ¹ýÀÌ ÇÊ¿äÇÑ °æ¿ì BacPAK¢â Baculovirus Expression System (Code 631402) Á¦Ç°ÀÇ ¸Å´º¾ó (¸µÅ©)¸¦ Âü°íÇϽñ⠹ٶø´Ï´Ù.
°ü·ÃÁ¦Ç°
- gp64 protein ¿°»öÀ» ÅëÇÑ titration : BacPAK Baculovirus Rapid Titer Kit
- qPCRÀ» ÀÌ¿ëÇÑ ºü¸¥ titration : BacPAK qPCR Titration Kit
- Insect cell line : IPLB-Sf21 Insect Cells
- All-in-One baculovirus system : BacPAK Baculovirus Expression System
- °íÈ¿À² ´Ü¹éÁú ¹ßÇöÀ» À§ÇÑ baculvirus DNA : flashBAC¢â Baculovirus Expression System
- ÃÊ°£´Ü His-Tagged protein Á¤Á¦ Ä÷³ : Capturem¢â His-Tagged Purification ½Ã¸®Áî (mini, maxi, plate µî)

Keyword :

°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
>
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
>
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
>
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
>
Baculovirus DNA & transfer vector set
Baculovirus DNA package kit
>
¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ..
Baculovirus Titer Kit
>
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
>
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
>
Plaque Assay ¼øÈ­°¡ ºÒÇÊ¿äÇÑ È¿À²ÀûÀÎ Recomb..
ProEasy Baculovirus Vectors
>
Sf9, Sf21 cell¿¡ ÃÖÀûÈ­µÈ Transfection reage..
ProFectin
>
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ­ È¿À² Çâ»ó
ProFold Baculovirus Vectors
>
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
>
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors