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¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ¿ª°¡ ÃøÁ¤

Baculovirus Titer Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631406
BacPAK Baculovirus Rapid Titer Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
5 ȸ
(Package)
1,007,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32503, x32504



  • Baculovirus ¹ßÇö ½ÇÇèÀ» ÃÖ´ë 6 ÀϱîÁö ´ÜÃà
  • ±î´Ù·Î¿î plaque ºÐ¼® °úÁ¤ ºÒÇÊ¿ä
  • ÀϹÝÀûÀ¸·Î ³Î¸® ÀÌ¿ëµÇ´Â (AcMNPV ±â¹Ý) baculovirus ¹ßÇö ½Ã½ºÅÛ¿¡µµ »ç¿ë °¡´É
BacPAK Baculovirus Rapid Titer Kit´Â baculovirus ¹ßÇö ½ÇÇè °úÁ¤ Áß ÀϹÝÀûÀ¸·Î °¡Àå ¸¹Àº ½Ã°£ÀÌ ¼Ò¿äµÇ´Â baculovirus stockÀÇ ¿ª°¡¸¦ ÃøÁ¤ÇÏ´Â ºü¸£°í °£´ÜÇÑ ¹æ¹ýÀ» Á¦°øÇÑ´Ù. ÀÌ kit´Â Ç¥ÁØ ¸é¿ªÇÐÀû ºÐ¼®¹ýÀ» »ç¿ëÇÏ¿© 48½Ã°£ À̳»¿¡ baculovirusÀÇ ¿ª°¡¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤ÇÏ´Â ¹Ý¸é, plaque°ú endpoint dilution ºÐ¼®¹ý°ú °°Àº ¹æ¹ýÀº 4-8 ÀÏÀÌ ¼Ò¿äµÈ´Ù.

BacPAK Baculovirus Expression System¿¡¼­ °¨¿°µÈ ¼¼Æ÷´Â plaque¸¦ Çü¼ºÇϱâ Àü¿¡ ¹ÙÀÌ·¯½º Ç׿øÀ» ¿À·¡ ¹ßÇö½ÃÅ°¹Ç·Î BacPAK Rapid Titer ºÐ¼®¹ýÀº ¾ÆÁÖ ÂªÀº ¹è¾ç ±â°£ ÈÄ ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù (1). ¶ÇÇÑ, ÃøÁ¤µÈ ¿ª°¡´Â ´Ù¸¥ ¹æ¹ýÀ¸·Î ÃøÁ¤µÈ °ª°ú ºñ±³ °¡´ÉÇÏ´Ù. ÀÌ kit´Â 104 pfu/ml ÀÌ»óÀÇ ¿ª°¡¸¦ °®´Â ¾î¶² ¹ÙÀÌ·¯½º¿Íµµ ÇÔ²² »ç¿ë °¡´ÉÇϸç, ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â AcMNPV ±â¹ÝÀÇ baculovirus ¹ßÇö ½Ã½ºÅÛ°úµµ ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ´Ù.

Rapid Titer ¸é¿ªºÐ¼®¹ýÀº ¹ÙÀÌ·¯½º¿¡ °¨¿°µÈ ¼¼Æ÷¸¦ Á¤È®ÇÏ°Ô È®ÀÎÇϱâ À§ÇÏ¿© AcMNPV envelope glycoprotein (gp64)¿¡ ´ëÇÑ primary monoclonal antibody¸¦ »ç¿ëÇÑ´Ù. 2Â÷ HRP-conjugated antibody´Â ±¤ÇÐ Çö¹Ì°æÀ¸·Î °¨¿°µÈ ¼¼Æ÷¸¦ ½Ã°¢È­ÇÏ°í ¹ÙÀÌ·¯½º ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖµµ·Ï ÇÑ´Ù.

BacPAK Rapid Titer Kit´Â 5 ȸÀÇ ¿ª°¡ ºÐ¼®À» À§ÇÑ ½Ã¾àÀ» Æ÷ÇÔÇÏ°í ÀÖ´Ù (ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â À¯±â ¿ë¸Å Á¦¿Ü).


Figure 1. Identification of infection foci in a lawn of Sf21 cells, using the BacPAK Baculovirus Rapid Titer Kit. Cells were immunostained with the gp64 primary antibody and an HRP-conjugated secondary antibody, and visualized by light microscopy.


Figure 2. Flow chart of the two-day BacPAK Baculovirus Rapid Titer Kit procedure.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Volkman, L. E. & Goldsmith, P. A. (1982) Appl. Envir. Microbiol. 44 (1):227-233.

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