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> TaKaRa Bradford Protein Assay Kit
Bradford ¹æ½ÄÀÇ ´Ü¹éÁú Á¤·® Kit
TaKaRa Bradford Protein Assay Kit
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Takara
T9310A
TaKaRa Bradford Protein Assay Kit
500ȸ
154,400¿ø
193,000¿ø
09.02 ~ 10.31
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
Bradford ¹æ½ÄÀÇ Protein assay Kit
°£´ÜÇÑ Á¶ÀÛÀ¸·Î ºü¸£°Ô ³ÐÀº ¹üÀ§, 1~ 1,000 ¥ìg/§¢, ´Ü¹éÁú Á¤·® °¡´É
Á¦Ç° ¼³¸í
TaKaRa Bradford Protein Assay Kit´Â coomassie dye¸¦ »ç¿ëÇÏ´Â Bradford ¹æ½ÄÀÇ Á¦Ç°À¸·Î, °£´ÜÇÑ ¹æ¹ýÀ¸·Î ½Å¼ÓÇÏ°Ô 1~1,000 ¥ìg/§¢ÀÇ ´Ü¹éÁú ³óµµ¸¦ Á¤·®ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº Coomassie dye°¡ ´Ü¹éÁú°ú °áÇÕÇÒ ¶§ Èí±¤µµ°¡ º¯ÇÏ´Â ¿ø¸®(465nm¡æ595nm)¸¦ ÀÌ¿ëÇÑ´Ù. ÀÌ º¯È´Â ¿ë¾× ³»ÀÇ ´Ü¹éÁúÀÇ ¾ç°ú ºñ·ÊÇϱ⠶§¹®¿¡, 585nmÀÇ Èí±¤µµ¸¦ ÃøÁ¤ÇÏ¿© ¿ë¾×ÀÇ ´Ü¹éÁú ³óµµ¸¦ Á¤·®ÇÒ ¼ö ÀÖ´Ù. ´Ü¹éÁú°ú »ö¼Ò º¹ÇÕü´Â ¹ÝÀÀ ½ÃÀÛ ÈÄ 5ºÐ¿¡¼ ÃÖ´ë 60ºÐ±îÁö ¾ÈÁ¤ÇÏ´Ù.
º» Á¦Ç°Àº »ùÇÿ¡ ȯ¿øÁ¦°¡ Á¸ÀçÇÏ¿©µµ ÃøÁ¤ °¡´ÉÇÏÁö¸¸, °è¸éÈ°¼ºÁ¦°¡ Á¸ÀçÇÑ´Ù¸é Á¤·®°ªÀÌ ºÎÁ¤È®ÇÏ°Ô µÇ¹Ç·Î ÁÖÀÇ°¡ ÇÊ¿äÇÏ´Ù(°øÁ¸¹°Áú¿¡ ÀÇÇÑ ¿µÇâ¿¡ ´ëÇؼ´Â <Ç¥.1> ÂüÁ¶).
¶ÇÇÑ ´Ü¹éÁú¿¡ µû¸¥ ¹ß»ö·üÀÌ ´Ù¸£±â ¶§¹®¿¡ ÁÖÀÇÇØ¾ß ÇÑ´Ù (´Ù¾çÇÑ ´Ü¹éÁúÀÇ ¹ß»ö ºñ±³´Â <Ç¥.2> Âü°í).
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º» Á¦Ç°Àº 1 §¢ ¹ÝÀÀ ½Ã 500ȸ, Microtiter plate¸¦ ÀÌ¿ëÇÑ 200 §¡ ¹ÝÀÀ ½Ã 2,500ȸÀÇ ÃøÁ¤À» ÇÒ ¼ö ÀÖ´Ù. Low-concentration ÇÁ·ÎÅäÄÝÀ» ÀÌ¿ëÇϸé 1 §¢ ¹ÝÀÀ ½Ã 1,000ȸ, 200 §¡ ¹ÝÀÀ ½Ã ÃÖ´ë 5,000ȸÀÇ ÃøÁ¤ÀÌ °¡´ÉÇÏ´Ù.
1. Bradford Dye Reagent
250 §¢¡¿2
2. BSA Standard Solution (2 mg/§¢)
*
1 §¢¡¿10
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: BSA Standard SolutionÀº ¾ÈÁ¤È¸¦ À§ÇØ 0.9% NaCl, 0.05% NaN
3
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4¡É, ³ÃÀå º¸°ü ÇϽʽÿÀ.
Á¦Ç° ÀÌ¿Ü¿¡ ÇÊ¿äÇÑ °Í
Spectrophotometer and compatible 1 §¢ cuvette
Microplate reader and compatible microplate
Benchtop centrifuge
Microtubes (2 §¢ or 1.5 §¢)
Spectrophotometer or microplate reader
´Ü¹éÁú Á¤·® »ùÇÿ¡ °øÁ¸ ¹°Áú¿¡ ÀÇÇÑ ¿µÇâ
º» Á¦Ç°Àº ȯ¿øÁ¦¿¡ ÀÇÇÑ ¿µÇâÀº Àû°Ô ¹ÞÁö¸¸, ³ôÀº ³óµµÀÇ °è¸éÈ°¼ºÁ¦´Â ÃøÁ¤¿¡ ¿µÇâÀ» ³¢Ä£´Ù.
<Ç¥. 1>Àº ÃøÁ¤¿¡ ¿µÇâÀ» ³¢Ä¡Áö ¾Ê´Â °øÁ¸¹°ÁúÀÇ ³óµµ¸¦ ³ªÅ¸³½ °ÍÀÌ´Ù.
<Ç¥. 1>¿¡´Â ÃøÁ¤¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â ³óµµ¸¦ Ç¥±âÇÏ¿´´Ù.
<Ç¥. 1> ´Ü¹éÁú Á¤·® »ùÇÃÀÇ °øÁ¸¹°Áú Çã¿ë ³óµµ
´Ü¹éÁú Á¾·ù¿¡ µû¸¥ ¿µÇâ
´Ü¹éÁú°ú »ö¼ÒÀÇ °áÇÕÀº ÁÖ·Î ´Ü¹éÁú Áß ¿°±â¼º ¾Æ¹Ì³ë»ê ¶Ç´Â ¹æÇâÁ· ¾Æ¹Ì³ë»ê°ú »ö¼ÒÀÇ °áÇÕ (ƯÈ÷ ¾Æ¸£±â´Ñ°úÀÇ °áÇÕ)¿¡ ÀÇÇÑ °ÍÀ̹ǷΠ´Ü¹éÁú Á¾·ù¿¡ µû¶ó ¹ß»ö·üÀÌ ´Ù¸£´Ù.
<±×¸². 1>Àº ÀϹÝÀûÀΠǥÁع°Áú·Î »ç¿ëµÇ´Â BSA (Bovine Serum Albumin)¿Í BGG (Bovine Gamma Globulin)ÀÇ Ç¥ÁØ °î¼±ÀÌ´Ù. ¶ÇÇÑ <Ç¥. 2>¿¡´Â 15Á¾·ùÀÇ ´Ü¹éÁúÀ» BSAÀÇ ¹ß»ö·ü°ú ºñ±³ÇÏ¿© ³ªÅ¸³»¾ú´Ù.
<±×¸². 1> BSA¿Í BGGÀÇ Ç¥ÁØ°î¼±
<Ç¥. 2> BSA¿¡ ´ëÇÑ ´Ù¾çÇÑ ´Ü¹éÁúÀÇ ¹ß»ö·ü ºñ±³
*Ratio: (°¢Á¾ ´Ü¹éÁúÀÇ Èí±¤µµ Æò±Õ°ª) / (BSAÀÇ Èí±¤µµÀÇ Æò±Õ°ª)
Keyword :
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
Western blotting HRP °ËÃâ ÈÇÐ ¹ß±¤ ±âÁú
HRP Substrate ½Ã¸®Áî
HRP-Juice
ÃÖÀûÀÇ buffer·Î ´Ü¹éÁú Àü±â¿µµ¿À» ºü¸£°Ô
ProSieve¢â EX Running Buffers
BCA ´Ü¹éÁú Á¤·® Kit
TaKaRa BCA Protein Assay Kit
Bradford ¹æ½ÄÀÇ ´Ü¹éÁú Á¤·® Kit
TaKaRa Bradford Protein Assay Kit
Tris-Glycine Powder
Western BLoT Blocking/Stripping Buffer
Western blot, ELISAÀÇ Ç×ü ¹ÝÀÀ ÃËÁøÁ¦
Western BLoT Immuno Booster
Western blot, ELISAÀÇ Ç×ü ¹ÝÀÀ ÃËÁøÁ¦ (1 Li..
Western BLoT Immuno Booster PF
HRPÇ¥½Ä 2Â÷ Ç×ü ´ë½Å¿¡
Western BLoT Rapid Detect v2.0