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Bradford ¹æ½ÄÀÇ ´Ü¹éÁú Á¤·® Kit

TaKaRa Bradford Protein Assay Kit

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Takara
T9310A
TaKaRa Bradford Protein Assay Kit
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500ȸ
121,000¿ø  T9310A_e.v1701Da.pdf


  • Bradford ¹æ½ÄÀÇ Protein assay Kit
  • °£´ÜÇÑ Á¶ÀÛÀ¸·Î ºü¸£°Ô ³ÐÀº ¹üÀ§, 1~ 1,000 ¥ìg/§¢, ´Ü¹éÁú Á¤·® °¡´É
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TaKaRa Bradford Protein Assay Kit´Â coomassie dye¸¦ »ç¿ëÇÏ´Â Bradford ¹æ½ÄÀÇ Á¦Ç°À¸·Î, °£´ÜÇÑ ¹æ¹ýÀ¸·Î ½Å¼ÓÇÏ°Ô 1~1,000 ¥ìg/§¢ÀÇ ´Ü¹éÁú ³óµµ¸¦ Á¤·®ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº Coomassie dye°¡ ´Ü¹éÁú°ú °áÇÕÇÒ ¶§ Èí±¤µµ°¡ º¯ÇÏ´Â ¿ø¸®(465nm¡æ595nm)¸¦ ÀÌ¿ëÇÑ´Ù. ÀÌ º¯È­´Â ¿ë¾× ³»ÀÇ ´Ü¹éÁúÀÇ ¾ç°ú ºñ·ÊÇϱ⠶§¹®¿¡, 585nmÀÇ Èí±¤µµ¸¦ ÃøÁ¤ÇÏ¿© ¿ë¾×ÀÇ ´Ü¹éÁú ³óµµ¸¦ Á¤·®ÇÒ ¼ö ÀÖ´Ù. ´Ü¹éÁú°ú »ö¼Ò º¹ÇÕü´Â ¹ÝÀÀ ½ÃÀÛ ÈÄ 5ºÐ¿¡¼­ ÃÖ´ë 60ºÐ±îÁö ¾ÈÁ¤ÇÏ´Ù.
º» Á¦Ç°Àº »ùÇÿ¡ ȯ¿øÁ¦°¡ Á¸ÀçÇÏ¿©µµ ÃøÁ¤ °¡´ÉÇÏÁö¸¸, °è¸éȰ¼ºÁ¦°¡ Á¸ÀçÇÑ´Ù¸é Á¤·®°ªÀÌ ºÎÁ¤È®ÇÏ°Ô µÇ¹Ç·Î ÁÖÀǰ¡ ÇÊ¿äÇÏ´Ù(°øÁ¸¹°Áú¿¡ ÀÇÇÑ ¿µÇâ¿¡ ´ëÇØ¼­´Â <Ç¥.1> ÂüÁ¶).
¶ÇÇÑ ´Ü¹éÁú¿¡ µû¸¥ ¹ß»ö·üÀÌ ´Ù¸£±â ¶§¹®¿¡ ÁÖÀÇÇØ¾ß ÇÑ´Ù (´Ù¾çÇÑ ´Ü¹éÁúÀÇ ¹ß»ö ºñ±³´Â <Ç¥.2> Âü°í).
³»¿ë
º» Á¦Ç°Àº 1 §¢ ¹ÝÀÀ ½Ã 500ȸ, Microtiter plate¸¦ ÀÌ¿ëÇÑ 200 §¡ ¹ÝÀÀ ½Ã 2,500ȸÀÇ ÃøÁ¤À» ÇÒ ¼ö ÀÖ´Ù. Low-concentration ÇÁ·ÎÅäÄÝÀ» ÀÌ¿ëÇϸé 1 §¢ ¹ÝÀÀ ½Ã 1,000ȸ, 200 §¡ ¹ÝÀÀ ½Ã ÃÖ´ë 5,000ȸÀÇ ÃøÁ¤ÀÌ °¡´ÉÇÏ´Ù.
1. Bradford Dye Reagent 250 §¢¡¿2
2. BSA Standard Solution (2 mg/§¢)* 1 §¢¡¿10
* : BSA Standard SolutionÀº ¾ÈÁ¤È­¸¦ À§ÇØ 0.9% NaCl, 0.05% NaN3 ¸¦ Æ÷ÇÔÇÑ´Ù.
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4¡É, ³ÃÀå º¸°ü ÇϽʽÿÀ.
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  • Spectrophotometer and compatible 1 §¢ cuvette
  • Microplate reader and compatible microplate
  • Benchtop centrifuge
  • Microtubes (2 §¢ or 1.5 §¢)
  • Spectrophotometer or microplate reader
´Ü¹éÁú Á¤·® »ùÇÿ¡ °øÁ¸ ¹°Áú¿¡ ÀÇÇÑ ¿µÇâ
º» Á¦Ç°Àº ȯ¿øÁ¦¿¡ ÀÇÇÑ ¿µÇâÀº Àû°Ô ¹ÞÁö¸¸, ³ôÀº ³óµµÀÇ °è¸éȰ¼ºÁ¦´Â ÃøÁ¤¿¡ ¿µÇâÀ» ³¢Ä£´Ù.
<Ç¥. 1>Àº ÃøÁ¤¿¡ ¿µÇâÀ» ³¢Ä¡Áö ¾Ê´Â °øÁ¸¹°ÁúÀÇ ³óµµ¸¦ ³ªÅ¸³½ °ÍÀÌ´Ù.
<Ç¥. 1>¿¡´Â ÃøÁ¤¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â ³óµµ¸¦ Ç¥±âÇÏ¿´´Ù.

<Ç¥. 1> ´Ü¹éÁú Á¤·® »ùÇÃÀÇ °øÁ¸¹°Áú Çã¿ë ³óµµ
´Ü¹éÁú Á¾·ù¿¡ µû¸¥ ¿µÇâ
´Ü¹éÁú°ú »ö¼ÒÀÇ °áÇÕÀº ÁÖ·Î ´Ü¹éÁú Áß ¿°±â¼º ¾Æ¹Ì³ë»ê ¶Ç´Â ¹æÇâÁ· ¾Æ¹Ì³ë»ê°ú »ö¼ÒÀÇ °áÇÕ (ƯÈ÷ ¾Æ¸£±â´Ñ°úÀÇ °áÇÕ)¿¡ ÀÇÇÑ °ÍÀ̹ǷΠ´Ü¹éÁú Á¾·ù¿¡ µû¶ó ¹ß»ö·üÀÌ ´Ù¸£´Ù.
<±×¸². 1>Àº ÀϹÝÀûÀΠǥÁع°Áú·Î »ç¿ëµÇ´Â BSA (Bovine Serum Albumin)¿Í BGG (Bovine Gamma Globulin)ÀÇ Ç¥ÁØ °î¼±ÀÌ´Ù. ¶ÇÇÑ <Ç¥. 2>¿¡´Â 15Á¾·ùÀÇ ´Ü¹éÁúÀ» BSAÀÇ ¹ß»ö·ü°ú ºñ±³ÇÏ¿© ³ªÅ¸³»¾ú´Ù.

<±×¸². 1> BSA¿Í BGGÀÇ Ç¥Áذ

<Ç¥. 2> BSA¿¡ ´ëÇÑ ´Ù¾çÇÑ ´Ü¹éÁúÀÇ ¹ß»ö·ü ºñ±³

*Ratio: (°¢Á¾ ´Ü¹éÁúÀÇ Èí±¤µµ Æò±Õ°ª) / (BSAÀÇ Èí±¤µµÀÇ Æò±Õ°ª)

Keyword :
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´Ü¹éÁú °ËÃâ, Á¤·®, ÇØ¼®
> HRP-Juice
ÃÖÀûÀÇ buffer·Î ´Ü¹éÁú Àü±â¿µµ¿°ú tranfer¸¦ ºü¸£..
> ProSieve¢â EX Running and Transfer Buffers
> Protein Medley
> Sodium Dodecyl Sulfate (SDS) Powder
BCA ´Ü¹éÁú Á¤·® Kit
> TaKaRa BCA Protein Assay Kit
Bradford ¹æ½ÄÀÇ ´Ü¹éÁú Á¤·® Kit
> TaKaRa Bradford Protein Assay Kit
> TaKaRa CBB Protein Safe Stain
> Tris-Glycine Powder
> Western BLoT Blocking/Stripping Buffer
Western blotting HRP°ËÃâ È­Çй߱¤±âÁú
> Western BLoT HRP Substrate ½Ã¸®Áî
Western blot, ELISAÀÇ Ç×ü ¹ÝÀÀ ÃËÁøÁ¦
> Western BLoT Immuno Booster
Western blot, ELISAÀÇ Ç×ü ¹ÝÀÀ ÃËÁøÁ¦ (1 Liquid..
> Western BLoT Immuno Booster PF
HRPÇ¥½Ä 2Â÷ Ç×ü ´ë½Å¿¡
> Western BLoT Rapid Detect v2.0
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