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Suspension CHO cellÀ» »ç¿ëÇÑ ´Ü±â°£ Ç×ü/´Ü¹éÁú ´ë·® ¹ßÇö ½Ã½ºÅÛ

CHOgro¢ç High Yield Expression System

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 6270
CHOgro¢ç High Yield Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
(Package)
°¡°Ý¹®ÀÇ   chogro-high-yield-expression-system-full-protocol.pdf
Mirus
MIR 6225
CHOgro¢ç Transfection and Titer Enhancer Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
(Package)
°¡°Ý¹®ÀÇ   chogro-high-yield-expression-system-full-protocol.pdf
Mirus
MIR 6200
CHOgro¢ç Expression Medium
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 L
°¡°Ý¹®ÀÇ   chogro-expression-system-protocol.pdf, chogro-high-yield-expression-system-full-protocol.pdf
Mirus
MIR 6210
CHOgro¢ç Complex Formation Solution
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
°¡°Ý¹®ÀÇ   chogro-expression-system-protocol.pdf, chogro-high-yield-expression-system-full-protocol.pdf
Mirus
MIR 6230
Poloxamer 188 Solution
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
°¡°Ý¹®ÀÇ   chogro-high-yield-expression-system-full-protocol.pdf
Mirus
MIR 6240
L-Glutamine Solution
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
°¡°Ý¹®ÀÇ   chogro-high-yield-expression-system-full-protocol.pdf
Mirus
MIR 5740
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 ml
°¡°Ý¹®ÀÇ   transit-pro-transfection-reagent-protocol.pdf
Mirus
MIR 5750
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 6201
CHOgro¢ç Expression Media, Dry Powder, Prepares 10 Liters
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ea*
°¡°Ý¹®ÀÇ   chogro-expression-medium-dry-powder-10-l.pdf
Mirus
MIR 6202
CHOgro¢ç Expression Media, 10 Liter Polybag
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 bag**
°¡°Ý¹®ÀÇ   chogro-expression-medium-qrp.pdf
Mirus
MIR 6250
Human IgG1 Expression Control
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ug
°¡°Ý¹®ÀÇ   human-igg1-expression-control-qrp_2019.pdf



* CHOgro¢ç Expression Media, Dry Powder, Prepares 10 LitersÀÇ ÃÖ¼Ò ¹ßÁÖ¼ö·®Àº 3°³ ÀÌ»óÀ̸ç, ³³±â±îÁö 8~10ÁÖ ¼Ò¿äµË´Ï´Ù.
** CHOgro¢ç Expression Media, 10 Liter PolybagÀÇ ÃÖ¼Ò ÁÖ¹® ¼ö·®Àº 10°³ ÀÌ»óÀ̸ç, ³³±â±îÁö 8~10ÁÖ ¼Ò¿äµË´Ï´Ù.

  • Suspension CHO cellÀ» ÀÌ¿ëÇÏ¿© ³ôÀº °æÁ¦¼ºÀ¸·Î °í¼öÀ² Ç×ü/´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛ
  • ±âÁ¸ Á¦Ç° (Code MIR 6260)Á¦Ç°¿¡ CHOgro¢ç Titer Enhancer°¡ Ãß°¡µÇ¾î ´Ü±â°£ ³» °í¼öÀ² Ç×ü/´Ü¹éÁú ¹ßÇö: Transfection 7ÀÏ ÈÄ °íÈ¿À² Ç×ü/´Ü¹éÁú ¹ßÇö °¡´É
  • °£ÆíÇÑ Protocol: Transfection ÈÄ enhancer ÷°¡ ¹× ¿Âµµ º¯°æ ¿Ü Ãß°¡ ½ÇÇè °úÁ¤ÀÌ ¾ø¾î, ¿À¿° ÃÖ¼ÒÈ­
  • Chemically defined, hydrolysate-free, animal origin-free medium
  • Á¦Ç° °øÁ¤ »ç¿ë½Ã º°µµÀÇ License °è¾à ÇÊ¿ä¾øÀ½
Á¦Ç° ¼³¸í
CHOgro¢ç High Yield Expression SystemÀº suspension CHO cellÀ» ÀÌ¿ëÇÏ¿© ´ë·®ÀÇ ´Ü¹éÁú ¹ßÇö¿¡ ÃÖÀûÈ­µÈ 2¼¼´ë Á¦Ç°ÀÌ´Ù. ±âÁ¸ CHOgro¢ç Expression System (Code MIR 6260)¿¡ CHOgro¢ç Titer Enhancer°¡ Ãß°¡µÇ¾î, »ó¿ëÈ­ Á¦Ç° Áß ÃÖ°í ¼öÁØÀ¸·Î ºü¸£°í ³ôÀº Ç×ü/´Ü¹éÁú ¹ßÇöÀ» ½ÇÇöÇÏ¿´´Ù. Suspension CHO cell¿ë ¹è¾ç¹èÁö ¹× ÷°¡Á¦, transfection ½Ã¾à µîÀÌ Æ÷ÇÔµÈ all-in-one systemÀ¸·Î ³ôÀº °æÁ¦¼ºÀ¸·Î °í¼öÀ² Ç×ü/´Ü¹éÁú »ý»êÀÌ °¡´ÉÇÏ´Ù.
º» Á¦Ç°¿¡ Æ÷ÇÔµÈ ¸ðµç ±¸¼º ¿ä¼Ò´Â µ¿¹° À¯·¡ ¼ººÐÀÌ Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù. ¶ÇÇÑ ±¸¼ºÇ° Áß CHOgro¢ç Titer EnhancerÀ» Á¦¿ÜÇϸé, ¸ðµÎ °³º°·Î ±¸¸Å°¡ °¡´ÉÇÏ¿© ´õ¿í ³ôÀº °æÁ¦¼ºÀ» ½ÇÇöÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. CHOgro¢ç High Yield Expression SystemÀ» ÀÌ¿ëÇÑ Ç×ü ¹ßÇö·® ºñ±³.
CHOgro¢ç High Yield Expression System°ú Expifectamine CHO Transfection Kit Ç×ü ¹ßÇö·®À» µ¿ÀÏÇÑ ±âº»±¸Á¶ÀÇ vector¸¦ ÀÌ¿ëÇÏ¿© Á¦ÀÛµÈ 6Á¾·ùÀÇ IgG1 Ç×ü ¹ßÇö plasmid¿¡¼­ ºñ±³ÇÏ¿´´Ù. CHOgro¢ç High Yield Expression SystemÀº CHOgro¢ç Expression Medium¿¡¼­ ¹è¾çµÈ FreeStyle CHO-S cells¿¡ 1 §¢´ç 1 §¶ÀÇ plasmid¸¦ TransIT¢ç-Pro¸¦ »ç¿ëÇÏ¿© transfection ÇÏ¿´´Ù (TransIT¢ç-PRO ½Ã¾à : plasmid = 1:1, ¼¼Æ÷ ¹Ðµµ 4¡¿106 cells/§¢). CHO cell¿¡ transfection complex¸¦ ÷°¡ÇÑ ÈÄ, CHOgro¢ç Titer Enhancer¸¦ Ãß°¡ÇÏ°í, ¹è¾ç ¿Âµµ¸¦ 37 ¡É¿¡¼­ 32 ¡É·Î º¯°æÇÏ¿© ¹è¾çÇÏ¿´´Ù. 
Expifectamine¢ç CHO Transfection Kit´Â ExpiCHO Expression Medium¿¡¼­ ¹è¾çµÈ ExpiCHO-S cells¿¡ 1 §¢ ´ç 1 §¶ÀÇ plasmid¸¦ transfectionÇÏ¿´´Ù (½Ã¾à : plasmid = 3.2 : 1, ¼¼Æ÷ ¹Ðµµ 6¡¿106 cells/§¢). ExpiCHO¢ç Expression SystemÀÇ Max titer protocol¿¡ µû¶ó 24 ½Ã°£ ÈÄ¿¡ Expifectamine¢ç CHO Enhancer¿Í Feed¸¦ ÷°¡ÇÏ¿© ¹è¾ç ¿Âµµ¸¦ 37 ¡É¿¡¼­ 32 ¡É·Î º¯°æÇÏ¿´°í, 5 ÀÏ° ExpiCHO¢ç Feed¸¦ ÷°¡ÇÏ¿´´Ù.
°¢°¢ ¹è¾ç¿¡´Â Thomson »çÀÇ 125 §¢ ÁøÅÁ ¹è¾ç¿ë Çöó½ºÅ© (25 §¢/º´)¸¦ »ç¿ëÇÏ¿´°í, 7ÀÏ ¹× 14 ÀÏ¿¡ ¹è¾ç¾× »óû Áß Ç×ü ³óµµ¸¦ ELISA ¹ýÀ¸·Î ÃøÁ¤ÇÏ¿´´Ù (Error bars = n3) . 
CHOgro¢ç High Yield Expression SystemÀº transfection ´çÀÏ¿¡ enhancer ÷°¡ ¹× ¹è¾ç ¿Âµµ¸¦ º¯È­½ÃÅ°´Â °£´ÜÇÑ protocol·Î, °æÀï Á¦Ç° ´ëºñ ´Ü±â°£ (7ÀÏ)/Àå±â°£ (14ÀÏ) ¹è¾ç ÈÄ ³ôÀº Ç×ü ¹ßÇö·®À» ½ÇÇöÇÏ¿´´Ù.


±×¸² 2. CHOgro¢ç Titer Enhancer·Î ÀÎÇÑ ¼¼Æ÷ ¼ºÀå ¹× µ¶¼º º¯È­ È®ÀÎ.
CHOgro¢ç Expression Medium¿¡ ¹è¾çµÈ FreeStyle CHO-S cells¿¡ TransIT¢ç-PRO¸¦ ÀÌ¿ëÇÏ¿© 1 §¢ ´ç 1 §¶ÀÇ plasmid¸¦ transfectionÇÏ¿´´Ù (TransIT¢ç-PRO : DNA = 1 : 1, ¼¼Æ÷ ¹Ðµµ 4¡¿106 cells/§¢). CHO cell¿¡ transfection complex¸¦ ÷°¡ÇÑ Á÷ÈÄ, CHOgro¢ç Titer Enhancer¸¦ Ãß°¡ÇÏ¿©, ¹è¾ç ¿Âµµ¸¦ 37 ¡É¿¡¼­ 32 ¡É·Î º¯°æ ÈÄ ¹è¾çÇÏ¿´´Ù. CHO cell¹è¾çÀº Thomson»çÀÇ 125 §¢ ÁøÅÁ ¹è¾ç¿ë Çöó½ºÅ© (25 §¢/º´)À» »ç¿ëÇÏ¿´´Ù.
Transfection ÈÄ 7 ÀϱîÁö flow cytometry·Î ¼¼Æ÷ ¼ö (½Ç¼±) ¹× »ýÁ¸À² (Á¡¼± : propidium iodide staining)À» ÃøÁ¤ÇÏ¿´´Ù (n=3). CHOgro¢ç Titer EnhancerÀÇ Ã·°¡·Î ÀÎÇÑ ¼¼Æ÷ Áõ½Ä ¹× µ¶¼ºÀº È®ÀεÇÁö ¾Ê¾Ò´Ù.


±×¸² 3. CHOgro¢ç Titer Enhancer ÷°¡·Î ÀÎÇÑ ´Ü¹éÁú ¹ßÇö·® ºñ±³.
¼­·Î ´Ù¸¥ Fc À¶ÇÕ ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â 2Á¾·ùÀÇ plasmid¸¦ »ç¿ëÇÏ¿© ÀϽÃÀû(transient)À¸·Î ´Ü¹éÁú ¹ßÇöÀ» ½Ç½ÃÇß´Ù. CHOgro¢ç Expression Medium¿¡¼­ ¹è¾çµÈ FreeStyle CHO-S cells¿¡ 1 §¢ ´ç 1 §¶ÀÇ plasmid¸¦ TransIT¢ç-PRO¸¦ ÀÌ¿ëÇÏ¿© transfectionÇÏ¿´´Ù (TransIT¢ç-PRO : DNA = 1 : 1, ¼¼Æ÷ ¹Ðµµ 4¡¿106 cells/§¢). CHO cell¿¡ transfection complex¸¦ ÷°¡ÇÑ Á÷ÈÄ CHOgro¢ç Titer Enhancer¸¦ Ãß°¡ÇÏ¿©, ¹è¾ç ¿Âµµ¸¦ 37 ¡É¿¡¼­ 32 ¡É·Î º¯°æÇÏ°í, ÄÚÆÃÇÏÁö ¾ÊÀº 6 well plate¿¡¼­ CHO cellÀ» ¹è¾çÇÏ¿´´Ù (2 §¢/well). ¹è¾ç 10ÀÏ ÈÄ, ¹è¾ç¾× Áß human IgG ³óµµ¸¦ ELISA·Î ÃøÁ¤ÇÏ¿´´Ù (Error bars = n3). CHOgro¢ç Titer EnhancerÀÇ Ã·°¡¿¡ ÀÇÇØ ´Ü¹éÁú ¹ßÇö·®ÀÌ 30~60% ÀÌ»ó »ó½ÂµÇ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.


±×¸² 4. CHOgro¢ç Titer Enhancer »ç¿ëÀ¸·Î Áõ°¡µÈ ¿ª°¡ ºñ±³.
CHOgro¢ç Expression Medium¿¡¼­ ¹è¾çµÈ CHO-S ¼¼Æ÷¿¡ 1 §¢ ´ç 1 §¶ÀÇ Fc À¶ÇÕ ´Ü¹éÁú ¹ßÇö plasmid¸¦ TransIT¢ç-PRO¸¦ ÀÌ¿ëÇÏ¿© transfectionÇÏ¿´´Ù (TransIT¢ç-PRO : DNA = 1 : 1). CHO cell¿¡ transfection complex¸¦ ÷°¡ Á÷ÈÄ, CHOgro¢ç Titer Enhancer¸¦ Ãß°¡ÇÏ¿© ¹è¾ç ¿Âµµ¸¦ 37 ¡É¿¡¼­ 32 ¡É·Î º¯°æÇÑ µÚ, 0.5 L ½ºÄÉÀÏ·Î ¹è¾çÇÏ¿´´Ù. ¹è¾ç 10ÀÏ¿¡ affinity chromatography·Î ´Ü¹éÁúÀ» Á¤Á¦ÇÏ¿©, CHOgro¢ç Titer Enhancer »ç¿ëÀ¸·Î ÀÎÇØ ´Ü¹éÁú ¹ßÇö·®ÀÌ Áõ°¡ÇÏ¿´À½À» È®ÀÎÇÏ¿´´Ù.
(µ¥ÀÌÅÍ Á¦°ø: Lytic Solutions, LLC)


±×¸² 5. ±âÁ¸ÀÇ Transfection ½Ã¾à°ú Ç×ü ¹ßÇö·® ºñ±³.
CHOgro¢ç High Yield Expression System (TransIT-PRO + CHOgro¢ç Titer Enhancer)¿Í PEI ±â¹Ý A»ç Á¦Ç° (½Ã¾à : DNA = 4 : 1) ¹× Linear PEI (25 kDa PEI : DNA = 6 : 1)À» ÀÌ¿ëÇÏ¿©, CHOgro¢ç Expression Medium¿¡¼­ ¹è¾çµÈ FreeStyle FreeStyle CHO-S cells¿¡ 1 §¢ ´ç 1 §¶ÀÇ IgG Ç×ü ¹ßÇö plasmid¸¦ transfectionÇÏ¿´´Ù. CHO cell¿¡ transfection complex¸¦ ÷°¡ Á÷ÈÄ CHOgro¢ç Titer Enhancer¸¦ Ãß°¡ÇÏ¿© ¹è¾ç ¿Âµµ¸¦ 37 ¡É¿¡¼­ 32 ¡É·Î º¯°æÇÑ µÚ, ¹è¾ç 14ÀÏÀÇ ¹è¾ç¾× Áß human IgG ³óµµ¸¦ ELISA¹ýÀ¸·Î ÃøÁ¤ÇÏ¿© ºñ±³ÇÏ¿´´Ù (Error bars = n3). ±× °á°ú, TransIT¢ç-PRO¿Í CHOgro¢ç Titer Enhancer¸¦ »ç¿ëÇÏ¿´À» ¶§ °¡Àå ³ôÀº Ç×ü ¹ßÇö·®À» È®ÀÎÇÏ¿´´Ù.

CHOgro¢ç High Yield Expression System (Code MIR 6270) ±¸¼º
±¸¼ºÇ°

¿ë·®

º°µµ±¸¸Å °¡´É

TransIT-PRO¢ç Transfection Reagent

1 §¢

MIR 5740

CHOgro¢ç Titer Enhancer Medium

20 §¢

º°µµ ±¸¸Å ºÒ°¡

CHOgro¢ç Expression Medium

2 ¡¿ 1000 §¢

MIR 6200

CHOgro¢ç Complex Formation Solution

100 §¢

MIR 6210

Poloxamer 188 Solution

100 §¢

MIR 6230

L-Glutamine Solution

100 §¢

MIR 6240


CHOgro¢ç Transfection and Titer Enhancer Kit (Code MIR 6225) ±¸¼º
±¸¼ºÇ°

¿ë·®

º°µµ±¸¸Å °¡´É

TransIT-PRO¢ç Transfection Reagent

1 §¢

MIR 5740

CHOgro¢ç Titer Enhancer Medium

20 §¢

º°µµ ±¸¸Å ºÒ°¡



´ë¿ë·® (10L) CHOgro¢ç Expression Media

±×¸² 6. ´ë·®ÀÇ CHO cell ¹è¾çÀ» À§ÇÑ ´ë¿ë·®ÀÇ CHOgro¢ç Expression Media.
Powder ÇüÅ (ÁÂ), Polybag ÇüÅ (¿ì)
°ü·Ã Á¦Ç°
°í¹ßÇö transfection reagent TransIT-PRO¢ç Transfection Reagent ÀÚ¼¼È÷ º¸±â
Endotoxin Á¦°Å¸¦ ÅëÇÑ ´Ü¹éÁú ¹ßÇö Çâ»ó MiraCLEAN¢ç Endotoxin Removal Kit ÀÚ¼¼È÷º¸±â
Transfection È¿À² È®Àοë LabelIT¢ç Plasmid Delivery Control, Cy¢ç 3 ÀÚ¼¼È÷º¸±â

Keyword :

Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
>
Suspension CHO cell¿ë ´ë·® ´Ü¹éÁú ¹ßÇö ½Ã½º..
CHOgro¢ç Expression System
>
Suspension CHO cellÀ» »ç¿ëÇÑ ´Ü±â°£ Ç×ü/´Ü..
CHOgro¢ç High Yield Expression System
>
´Ü¹éÁú disulfide BondÀÇ ÀçÁ¶ÇÕ¿¡
Corystein¢â£¨Purothionin£©
>
¹«¼¼Æ÷ ´Ü¹éÁú ÇÕ¼º°è
Human Cell-Free Protein Expression System
>
Human cell (HEK293)¿¡¼­ ´Ü¹éÁúÀ» °í¹ßÇö
pHEK293 Ultra Expression Vector