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Real Time PCR
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TB Green °ËÃâ(Clontech)
> Terra qPCR Direct TB Green¢â Premix
TB Green¢â
Terra qPCR Direct TB Green¢â Premix
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Clontech
638319
Terra qPCR Direct TB Green¢ç Premix
200 rxns
462,000¿ø
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Product index
Overview
FASTest Real Time RT-P..
Dye-based Real Time PC..
Real Time PCR¿ë cDNA ..
One-step Real Time PCR..
Probe °ËÃâ¿ë Real Time..
Real Time PCR accessor..
The Best Tool for qPCR
qPCR Preamplification
PreAmp Master Mix
Real Time PCR ±â±â
Thermal Cycler Dice¢ç ..
Thermal Cycler Dice¢ç ..
CronoSTAR¢â 96 Real-Ti..
SmartChip¢â Real Time ..
cDNA ÇÕ¼º (Real Time PCR ..
gDNA Á¦°Å& RT ½Ã¾à (qP..
5x RT Master Mix (qPCR..
RT components (qPCR¿ë)
[New] ½Ã°£´ÜÃà, gDNA ..
»ùÇà dilution ½Ã¾à (qP..
EASY Dilution II (for ..
Intercalator TB Green °ËÃâ
Standard qPCR Mix
Long target¿ë qPCR Mix
Fast qPCR Mix
Dimer eraser qPCR Mix
GC rich qPCR Mix
Crude »ùÇÿë qPCR Mix
Premixed 1 step qRT-PC..
Specific 1 step qRT-PC..
Standard 1 step qRT-PC..
External Standard Kit ..
Transgene Detection Pr..
[New] Fast, UNG, dye p..
Dual-Labeled Probe °ËÃâ
Probe qPCR Mix MultiPl..
Probe qPCR Mix (¶Ç´Â U..
2 x all premix 1 step ..
Premix Ex Taq¢â (Probe..
[New] ½Ã°£´ÜÃà, gDNA ..
One Step PrimeScript¢â..
gDNA Á¦°Å& RT ½Ã¾à (qP..
5x RT Master Mix (qPCR..
RT components (qPCR¿ë)
PrimeDirect¢ç Probe RT..
Cell direct qPCR
RNA ÃßÃâ¾øÀÌ cell dire..
qPCR/Western¿ë cell ly..
1 step qRT-PCR¿ë cell ..
qPCR¿ë cell lysate Á¶..
Whole Transcriptome Áõ..
TB Green °ËÃâ(Clontech)
TB Green¢ç Advantage q..
Terra qPCR Direct TB G..
CycleavePCR °ËÃâ
Cycling Probe ¹ý¿¡ ´ë..
CycleavePCR Core Kit
CycleavePCR GBS Capsul..
CycleavePCR Streptococ..
CycleavePCR¢â Reaction..
CycleavePCR¢â Salmonel..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (Real ..
Bacteria (
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Provirus Copy Number D..
Transgene Detection Pr..
DNA Á¤Á¦ ¾øÀÌ °ð¹Ù·Î Real Time PCR Àû¿ë
Real Time qPCR Master Mix-Terra qPCR Direct TB Green¢â Premix
Terra qPCR Direct TB Green¢â Premix
´Â 2X master mix·Î ¸¶¿ì½º ²¿¸®³ª ±Í Á¶Á÷, ¶Ç´Â ½Ä¹°ÀÇ ÀÙ Á¶Á÷À» Á¤Á¦ ¾øÀÌ crude extract ÇüÅ·ΠReal Time PCR (qPCR)¿¡ °ð¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.
qPCR¿¡ »ç¿ëµÇ´Â Terra PCR Direct Polymerase´Â whole cell, crude cell lysate ¶Ç´Â tissue extract±×¸®°í Á¤Á¦µÈ DNA¿¡¼µµ ¶Ù¾î³ ÁõÆø È¿À²À» º¸À̵µ·Ï ÃÖÀûÈµÈ non-Taq polymerase·Î premix ÇüÅ·ΠÁ¦°ø µÈ´Ù.
±âº»ÀÌ µÇ´Â Terra Polymerase´Â GC ÇÔ·®ÀÌ 70% ÀÌ»óÀÎ À¯ÀüÀÚ Àû¿ë ½Ã¿¡µµ ¶Ù¾î³ ÁõÆø È¿À²À» ³ªÅ¸³¿À» È®ÀÎ ÇÏ¿´´Ù(Figure 2). º» premix ½Ã¾àÀº TB Green¢â Green I dye »Ó¸¸ ¾Æ´Ï¶ó monoclonal antibody¸¦ »ç¿ëÇÏ¿© hotstart PCRÀ» ÁøÇàÇϱ⠶§¹®¿¡ 10ºÐ°£ÀÇ predeanture time ¾øÀ̵µ automatic hot start ¹ÝÀÀÀÌ °¡´ÉÇÏ´Ù.
Terra qPCR Direct TB Green¢â Premix´Â fluorescence signalÀ» normalizationÇϴµ¥ ÀÌ¿ëµÇ´Â 2Á¾·ùÀÇ ROX reference Dye¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù.
ROX Reference Dye LSR
- ROX Reference Dye LSR´Â excitation source°¡ 488 nm laserÀÎ ±â±â¿¡ ÀûÇÕÇÏ´Ù.
ROX Reference Dye LMP
- ROX Reference Dye LMP´Â excitation source°¡ lamp ¶Ç´Â LEDÀÎ ±â±â¿¡ ÀûÇÕÇÏ´Ù.
ROX Reference Dye ÷°¡ ºÒÇÊ¿ä
Takara»çÀÇ Thermal Cycler Dice Real Time System II ¶Ç´Â Cepheid »çÀÇ Smart Cycler System, Roche»çÀÇ LightCycler¿¡´Â º°µµ·Î ÷°¡ÇÒ ÇÊ¿ä°¡ ¾ø´Ù.
Figure 1. Real-time PCR with crude extracts-Terra qPCR Direct TB Green¢â Premix versus a conventional 2X qPCR premix.
Real-time PCR was performed using undiluted, 4X diluted, and 16X diluted crude alkaline-heat extracts of mouse spleen or cow muscle (beef foodstuff), and either Terra qPCR Direct TB Green¢â Premix or a conventional qPCR premix. Using the manufacturer's recommended conditions for each enzyme mix, a 165 bp region of the ¥â-globin gene Hbb-b1 was amplified from the mouse spleen extract (Panel A), and a 289 bp region of the cytochrome c oxidase gene (COX1) was amplified from the beef extract ( Panel B). Data generated by Terra qPCR Direct TB Green¢â Premix corresponded to the theoretical quantities of each gene, while the conventional product was clearly affected by inhibitors present in the crude samples.
Figure 2. Real-time PCR of GC-rich targets-Terra qPCR Direct TB Green¢â Premix versus conventional 2X qPCR premixes.
Targets with GC-content greater than 70% were amplified by real-time PCR using either human testis cDNA (equivalent to 50 ng-5 pg of total RNA; Panel A) or human genomic DNA (100 ng-10 pg; Panel B) as template, and either Terra qPCR Direct TB Green¢â Premix (triangles) or one of two conventional TB Green¢â premixes (one specifi¡©cally for GC-rich targets; see figure legend). Using the manufacturer's recommended conditions for each enzyme mix, gene-specific primers were used to amplify portions of the jun-D proto-oncogene (JUND), the BTB domain-containing protein 6 gene (BTBD6), and the cyclin I gene (CCNI) from the cDNA (Panel A); and a portion of the ¥â-actin CpG island (ACTB_CpG) from the genomic DNA (Panel B). The resulting Ct values were plotted against the initial quantity of DNA used in each assay. Terra qPCR Direct TB Green¢â Premix was the only premix able to consistently amplify all of the targets assayed.
Applications
Perform direct real-time PCR from crude extracts, and dirty samples without having to purify template.
Components
2X Terra qPCR Direct TB Green¢â Premix* : Contains Terra PCR Direct Polymerase (w/ hot start Ab), dNTP Mixture, Mg
2+
and TB Green¢â Green I dye.
ROX Reference Dye LSR (50X)
ROX Reference Dye LMP (50X)
Storage Conditions
Store all components at -80¡É after thawing, store the kit-protected from light at 4¡É and use within 6 months.
Do not refreeze!
Keyword :
TB Green °ËÃâ(Clontech)
TB Green¢ç Advantage qPCR Premix
TB Green¢â
Terra qPCR Direct TB Green¢â Premix