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Multiplex probe qPCR ½Ã¾à (UNG Æ÷ÇÔ)

Probe qPCR Mix MultiPlus

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR393A
Probe qPCR Mix MultiPlus
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
400ȸ (25 §¡ ¹ÝÀÀ ½Ã)
266,400¿ø 
333,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR393S_RR393A_e.v2406Da.pdf
Takara
RR393B
Probe qPCR Mix MultiPlus
°ü·ÃÇмú ±¸¸ÅÇϱâ
800ȸ (RR393A x 2)
600,000¿ø 

  • ´Ù¼öÀÇ Å¸°ÙÀ» µ¿½Ã¿¡ °ËÃâÇÏ´Â multiplex ¹ÝÀÀ¿¡ ÃÖÀû
  • °í¼Ó¹ÝÀÀÀ¸·Î qPCR ½Ã°£ ´ÜÃà : denaturation 1ÃÊ, extension 20ÃÊ
  • ³»¿­¼º Tli RNase H°¡ Æ÷ÇÔµÇ¾î ¹ÝÀÀ ÀúÇظ¦ ±ØÇѱîÁö ¾ïÁ¦
  • UNG »ç¿ëÀ¸·Î carryover contamination ¹æÁö
Á¦Ç°¼³¸í
Probe qPCR Mix MultiPlus´Â 5¡¯-nuclease¹ý¿¡ ÀÇÇÑ probe °ËÃâ Àü¿ë Real Time PCR ½Ã¾àÀÌ´Ù.
º» Á¦Ç°Àº Æø³ÐÀº ¹üÀ§¿¡¼­ ºü¸£°í ƯÀ̼ºÀÌ ³ôÀº ÁõÆøÀ» ½ÇÇöÇϱâ À§ÇØ antibody¸¦ ÀÌ¿ëÇÑ hot start PCR È¿¼Ò¿Í Real Time PCR¿ë buffer¸¦ ÃÖÀûÈ­ÇÏ¿´´Ù. ¶ÇÇÑ ¿©·¯ Ÿ°ÙÀ» µ¿½Ã¿¡ °ËÃâÇÏ´Â multiplex ¹ÝÀÀ ¿ª½Ã °í°¨µµÀÇ °ËÃâ°ú ³ôÀº Á¤·®¼ºÀ¸·Î ÀçÇö¼º ³ôÀº ÁõÆøÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
2x premix ŸÀÔÀÇ ½Ã¾à¿¡´Â ³»¿­¼º Tli RNase H¿Í UNG(Uracil-N-Glycosylase)°¡ Æ÷ÇԵǾî cDNA ÁÖÇü Áß ÀÜÁ¸ÇÏ´Â mRNAÀÇ PCR ¹ÝÀÀ ÀúÇظ¦ ±ØÇѱîÁö ¾ïÁ¦ÇÏ°í carryover contamination¿¡ ÀÇÇÑ ÁõÆøÀ» ¹æÁöÇÑ´Ù.
³»¿ë (Code RR393A, 25 §¡ ¹ÝÀÀ½Ã 400ȸ ¹ÝÀÀºÐ)

Probe qPCR Mix MultiPlus (2¡¿conc.)*1

1 §¢ x 5

ROX Reference Dye (50¡¿conc.) *2

200 §¡

ROX Reference Dye II for RR393 (100¡¿conc.) *2

200 §¡


*1 PCRÈ¿¼Ò, dNTP Mixture, Mg2+, Tli RNaseH, UNG¸¦ Æ÷ÇÔÇÑ´Ù.
*2Applied BiosystemsÀÇ Real Time PCR ±â±â µî well °£ Çü±¤ ½Ã±×³Î º¸Á¤À» ¼öÇàÇÏ´Â ÀåÄ¡¿¡¼­ ºÐ¼®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
  • ROX Reference Dye (50¡¿)¸¦ ÷°¡ÇÏ´Â ±âÁ¾ (ÃÖÁ¾ ³óµµ 1 ¡¿·Î »ç¿ë)
    StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)

  • ROX Reference Dye II for RR393 (100¡¿)¸¦ ÷°¡ÇÏ´Â ±âÁ¾ (ÃÖÁ¾ ³óµµ 1 ¡¿·Î »ç¿ë)
    QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific)
    Applied Biosystems7500 Fast Real-Time PCR System (Thermo Fisher Scientific)

  • ÷°¡ÇÒ ÇÊ¿ä°¡ ¾ø´Â ±âÁ¾
    CronoSTAR 96 Real-Time PCR System (Code 640231/640232)
    Thermal Cycler Dice Real Time System ½Ã¸®Áî (Code TP950/TP970/TP980/TP990, TP900/TP960: Á¾¸Å)
    CFX96 Real-Time PCR Detection System (Bio-Rad)
º¸Á¸ - 20 ¡É
°ü·ÃÁ¦Ç°
- ´Ù¾çÇÑ »ùÇÃÀÇ gDNA Á¤Á¦: TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
- gDNA Á¦°Å Ä÷³À¸·Î °í¼øµµ RNA Á¤Á¦: TaKaRa MiniBEST Universal RNA Extraction Kit
- ´õ¿í ºü¸£°í Æí¸®ÇØÁø gDNA Á¦°Å & RT premix ½Ã¾à (qPCR¿ë): PrimeScript¢â FAST RT reagent Kit with gDNA Eraser
- 5x RT Master Mix (qPCR¿ë): PrimeScript¢â RT Master Mix (Perfect Real Time)
- ÇÕ¸®ÀûÀÎ °¡°Ý, °¡¼ººñ ³ôÀº qPCR ±â±â CronoSTAR¢â 96 Real-Time PCR System(4ch)

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