´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > CycleavePCR °ËÃâ > CycleavePCR¢â Salmonella Detection Kit Ver.2.0
Cycling probe¸¦ ÀÌ¿ëÇÑ Salmonella±Õ °ËÃâ

CycleavePCR¢â Salmonella Detection Kit Ver.2.0

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
CY205
CycleavePCR Salmonella Detection Kit Ver.2.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
442,400¿ø 
553,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
CY205_e.v1903Da.pdf

Á¦Ç°¼³¸í
»ì¸ð³×¶ó±ÕÀº ´ëÀå±ÕÀ̳ª Àû¸®±Õ°ú °°Àº ±×·ì¿¡ ¼ÓÇÏ´Â ¼¼±ÕÀ¸·Î, ÀÚ¿¬°è¿¡ ³Ð°Ô ºÐÆ÷ÇÏ°í ÀÖÀ¸¸ç, ¾à 2,500Á¾·ù ÀÌ»óÀÇ Ç÷ûÇüÀÌ ¾Ë·ÁÁ® ÀÖ´Ù. ÇöÀç ÀÌ Áß¿¡¼­µµ ƯÈ÷, Salmonella Enteritidis¿¡ ÀÇÇÑ ½ÄÁßµ¶ÀÌ ±Þ°ÝÇÏ°Ô Áõ°¡ÇÏ°í ÀÖ´Ù. º» Á¦Ç°Àº ´ëºÎºÐÀÇ »ì¸ð³Ú¶ó±ÕÀÌ º¸À¯ÇÏ°í ÀÖ´Â À¯ÀüÀÚÀÇ ÇϳªÀΠħÀÔ¼º ÀÎÀÚ°ü·Ã À¯ÀüÀÚ invA¸¦ Real Time PCR ÀåÄ¡¸¦ ÀÌ¿ëÇÏ¿© °ËÃâÇÑ´Ù.
Hot Start PCR¿ë È¿¼ÒÀÎ TaKaRa Ex Taq HS¸¦ »ç¿ëÇÏ¿©, primerÀÇ misprimingÀ̳ª primer dimer À¯·¡ÀÇ ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¸·À» ¼ö°¡ ÀÖ¾î °í°¨µµ °ËÃâÀÌ °¡´ÉÇÏ´Ù. ÁõÆø»ê¹°ÀÇ °ËÃâ¿¡´Â Cycling probe¹ýÀ» »ç¿ëÇÏ°í ÀÖ´Ù.
º» Á¦Ç°¿¡´Â »ì¸ð³×¶ó±ÕÀÇ Ä§ÀÔ¼º ÀÎÀÚ°ü·Ã À¯ÀüÀÚ invA °ËÃâ¿ë FAM Ç¥½Ä probe, internal control ¹× internal control °ËÃâ¿ë ROX Ç¥½Ä probe°¡ Æ÷ÇԵǾî ÀÖ´Ù.
µÎ ÆÄÀåÀ» µ¿½Ã¿¡ ¸ð´ÏÅ͸µ ÇÔÀ¸·Î¼­ ÇϳªÀÇ tube¿¡¼­ invA À¯ÀüÀÚÀÇ °ËÃâ°ú internal control DNAÀÇ °ËÃâ¿¡ ÀÇÇÑ false negative reactionÀ» È®ÀÎÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ Ver 2.2Àº ±âÁ¸ Á¦Ç°ÀÇ °¢ ±¸¼ºÇ°À» premixÈ­ÇÏ¿© Æí¸®¼ºÀ» ³ô¿´´Ù.

<ÁÖÀÇ>
º» Á¦Ç°Àº ÀϺΠ»ç¾çÀ» ¾÷±×·¹À̵åÇϸç Lot. AK701 Á¦Ç°ºÎÅÍ Smart Cycler II System (Cepheid)ÀÇ »ç¿ëÀ» ±ÇÀåÇÏÁö ¾Ê½À´Ï´Ù.
Thermal Cycler Dice Real Time System°ú Applied Biosystems 7500 Real-Time PCR System(Thermo Fisher Scientific) µî ±â±â »ç¿ëÀ» ÃßõÇÕ´Ï´Ù.
³»¿ë (50ȸ; 25 §¡ ¹ÝÀÀ ½Ã)

¡¡

 

±¸¼ºÇ°¸í

¿ë·®

¡Ü

1.

2¡¿Cycleave Reaction Mixture (2¡¿conc.)

625 §¡

¡Ü

2.

SIN Primer/Probe Mix (FAM, ROX)* (5¡¿conc.)

250 §¡

¡Û

3.

dH2O

1 §¢

¡Ü

4.

SIN Positive Control

150 §¡ (30ȸ ºÐ)


* Fluorescent-labeled probe¸¦ Æ÷ÇÔÇϹǷÎ, Â÷±¤¿¡ ÁÖÀÇÇÒ °Í.
   ÇÁ¶óÀ̸Ӵ SHIMADZU CORPORATION¿¡¼­ Á¦Á¶
º¸Á¸ -20¡É
Primer Á¤º¸

Primer¸í

ÁõÆø »çÀÌÁî
(Ÿ°Ù)

ÁõÆø »çÀÌÁî
(ÀÎÅͳΠÄÁÆ®·Ñ)

SIN1/SIN2

378 bp

122 bp


Keyword :

CycleavePCR °ËÃâ
>
Cycling Probe ¹ýÀ» ÀÌ¿ëÇÑ Real Time PCR ¿ë ..
CycleavePCR Core Kit
>
Cycling probe Àü¿ë qPCR premix ½Ã¾à
CycleavePCR¢â Reaction Mix
>
Cycling probe¸¦ ÀÌ¿ëÇÑ Salmonella±Õ °ËÃâ
CycleavePCR¢â Salmonella Detection Kit Ver.2.0