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°ü·ÃÇؼ ¹®Àdz»¿ë ÀÖÀ¸½Ã¸é °í°´Áö¿ø¼¾ÅÍ (02-2081-2510,
support@takara.co.kr)·Î ¹®Àǹٶø´Ï´Ù.
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10¡¿ CycleavePCR Buffer |
125 §¡ |
dNTP Mixture (°¢2.5 mM) |
150 §¡ |
Mg2+ solution (25 mM) |
250 §¡ |
TaKaRa Ex Taq HS (5 U/§¡) |
12.5 §¡ |
Tli RNase H II (200 U/§¡) |
25 §¡ |
Positive Control (104 copies/§¡)*1 |
10 §¡ |
Positive Control Primer mix (°¢10 §) |
10 §¡ |
Positive Control probe (FAM Ç¥½Ä)*2 (25¡¿) |
10 §¡ |
dH2O |
700 §¡ |
*1 Positive ControlÀº Çö󽺹̵åÀ̸ç, copy¼ö´Â A260ÀÇ Èí±¤µµ·ÎºÎÅÍ È¯»êÇÑ ÁöÇ¥·Î
½ÇÁ¦ÀÇ ºÐÀÚ¼ö¸¦ ³ªÅ¸³»°í ÀÖ´Â °ÍÀº ¾Æ´Õ´Ï´Ù.
*2 ¹ÝÀÀ control °ËÃâ¿ëÀÇ ÇÁ·ÎºêÀÔ´Ï´Ù. Çü±¤ Ç¥½Ä ÇÁ·Îºê´Â Â÷±¤¿¡ À¯ÀÇÇϽñ⠹ٶø´Ï´Ù.
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º» Á¦Ç°Àº Cycling probe¸¦ ÀÌ¿ëÇÏ´Â Real Time PCR Àü¿ë Å°Æ®ÀÌ´Ù. ½Å¼Ó¼º°ú Á¤·®¼ºÀÌ ¶Ù¾î³ Real Time PCR ¹ý°ú ƯÀ̼ºÀÌ
¸Å¿ì ³ôÀº °ËÃâ¹ýÀÎ Cycling probe¹ýÀ» Á¶ÇÕÇÏ¿© Ÿ°ÙÀÇ °ËÃâÀ̳ª Á¤·®, SNPÀÇ °ËÃâ µîÀ» Á¤È®ÇÏ°í °£ÆíÇÏ°Ô ÇÒ ¼ö ÀÖ´Ù.
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- Real Time PCR°ú Cycling probe¹ýÀ¸·Î ½Å¼Ó, Á¤È®ÇÏ°Ô PCR ¹ÝÀÀ °á°ú¸¦
¾Ë ¼ö ÀÖ´Ù. ÇÑ ¿°±âÀÇ Â÷À̸¦ ½Äº°ÇÏ´Â SNP Çؼ®À» À§ÇÑ °ËÃâ°è¸¦ ±¸ÃàÇÏ´Â
Àϵµ °¡´ÉÇÏ´Ù.
- PCR¿¡´Â Hot Start¿ë È¿¼Ò TaKaRa Ex Taq HS¸¦ ÀÌ¿ëÇÏ°í ÀÖ´Ù. Real Time
PCR ¿ëÀ¸·Î ÃÖÀûÈ µÇ¾î ÀÖ¾î, ÁõÆø È¿À²ÀÌ ÁÁ°í, °í°¨µµ °ËÃâÀÌ °¡´ÉÇÏ´Ù.
º» Á¦Ç° ¿Ü¿¡ ÇÊ¿äÇÑ ½Ã¾à, ±â±â (ÁÖ¿äÇÑ °Í¸¸ Ç¥±â)
1. Real Time PCR ±â±â (Authorized instruments)
Thermal Cycler Dice Real Time System II (Code TP900/TP960)
Smart Cycler II System (Code SC200Nµî)
Applied Biosystems 7500 Real-Time PCR System (Life Technologies)¿Ü
2. Thermal Cycler Dice Real Time¿ë plate (Code NJ400)
Smart Cycler tube (Code SC910A/C)¿Ü
3. PCR¿ë primer*
4. Cycling probe*
5. Micropipette ¹× tip (¸ê±Õó¸®ÇÑ °Í)
*Primer ¹× Cycling probe µðÀÚÀÎÀº ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (02-2081-2510, support@takara.co.kr)·Î ¹®Àǹٶø´Ï´Ù.