(25μ l반응, 100회분)

TB Green™ Premix Ex Taq GC^*1

2×conc.

625 μl×2

TUF Primer Mix

5×conc.

250 μl×2

dH₂O

1 ml

ROX Reference Dye^*2

50×conc.

50 μl

ROX Reference Dye II^*2

50×conc.

50 μl

TUF Positive Control

1×105 copies/μl

100 μl

EASY Dilution (for Real Time PCR)

1ml×2