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Àúµ¶¼º No.1, µ¶¼º¿¡ ¹Î°¨ÇÑ ¼¼Æ÷µµ °íÈ¿À²·Î plasmid µµÀÔ °¡´É

TransIT¢ç-LT1 Transfection Reagent

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Mirus
MIR 2304
TransIT-LT1 Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
0.4 ml
°¡°Ý¹®ÀÇ   ml001_transit_lt1_transfection_reagent.pdf
Mirus
MIR 2300
TransIT-LT1 Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 2305
TransIT-LT1 Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿5
°¡°Ý¹®ÀÇ  
Mirus
MIR 2306
TransIT-LT1 Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿10
°¡°Ý¹®ÀÇ  



  • ³·Àº ¼¼Æ÷ µ¶¼º (Low Toxicity)À¸·Î transfection ÈÄ, ¼¼Æ÷ ³óµµ À¯Áö ¹× ½ÇÇè ÆíÇ⼺ °¨¼Ò
  • ´Ù¾çÇÑ ¼¼Æ÷¿¡ DNA¸¦ °íÈ¿À²·Î µµÀÔ °¡´É
  • ´ÜÀÏ ¶Ç´Â ´Ù¼öÀÇ plasmid µµÀÔ °¡´É
  • iPSCÀÇ reverse transfection ¿¡µµ ÃÖÀû: ½ÇÇè °¡À̵å¶óÀÎ º¸±â (Ŭ¸¯)
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TransIT¢ç-LT1 Transfection Reagent¸¦ »ç¿ëÇÏ¸é °íÈ¿À²·Î plasmid DNA¸¦ ´Ù¾çÇÑ mammalian cell type¿¡ µµÀÔÇÒ ¼ö ÀÖ´Ù. ÀÌ Á¦Ç°Àº ³·Àº ¼¼Æ÷µ¶¼º°ú serum compatible transfectionÀÌ Æ¯Â¡À¸·Î transfection ÈÄ º°µµÀÇ media change°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù´Â ÀåÁ¡ÀÌ ÀÖ´Ù. Transient transfection°ú stable transfectionÀ» Æ÷ÇÔÇØ gene expression, viral production, shRNA expression, promoter analysis µî ´Ù¾çÇÑ applicationÀ» Àû¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë
(#MIR 2300 ±âÁØ; 1.0 §¢)
±¸¼ºÇ°

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TransIT¢ç-LT1 Transfection Reagent

1 x 1.0 §¢

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°ü·ÃÁ¦Ç°
- Á¦ÇÑÈ¿¼Ò site¸¦ °í·ÁÇÏÁö ¾Ê´Â PCR cloing ¿øÁ¶ In-Fusion¢ç Snap Assembly Master Mix (Code 638947)
- Mycoplasma ¿À¿° È®ÀÎ ½Ã¾à
   : Nested PCR·Î Á¤¹Ð °ËÃâ TaKaRa PCR Mycoplasma Detection Set
   : °í°¨µµÀÇ cycling probe qPCR ÀÌ¿ë CycleavePCR Mycoplasma Detection Kit
   : Luminometer·Î 20ºÐ¸¸¿¡ °ËÃâ MycoAlert PLUS mycoplasma detection kit
- ¿ëµµº°/»ö±òº°·Î ¼±ÅÃ: Çü±¤´Ü¹éÁú vector
- Mammalian cell selection¿ë: Antibiotics
½ÇÇè µ¥ÀÌÅÍ

±×¸² 1. Ÿ»çÁ¦Ç° ´ëºñ ³ôÀº µµÀÔÈ¿À²°ú ³·Àº ¼¼Æ÷µ¶¼ºÀ» ³ªÅ¸³»´Â TransIT¢ç-LT1 Transfection Reagent. HepG2 ¼¼Æ÷¿¡ luciferase expression plasmid¸¦ °¢°¢ÀÇ transfection ½Ã¾àÀ» Á¦Á¶»çÀÇ ±ÇÀå Á¶°ÇÀ¸·Î µµÀÔÇÑ °á°úÀÌ´Ù (°¢ reagent : DNAÀÇ ºñÀ²Àº ¾Æ·¡¿¡ Ç¥±âµÇ¾î ÀÖÀ½). 96-well plate¿¡¼­ °¢ well¿¡ 0.1 §¶ÀÇ plasmid DNA¸¦ transfection ÇÏ¿´°í, 24½Ã°£ µÚ luciferase activity (¸·´ë ±×·¡ÇÁ)¿Í lactate dehydrogenase (LDH) level (¼± ±×·¡ÇÁ)À» ÃøÁ¤ÇÏ¿´´Ù. ¿©±â¼­ LDH levelÀº ½ÃÁß¿¡ ÆǸŵǴ colorimetric assay Á¦Ç°À¸·Î ºÐ¼®ÇÏ¿© transfection ÇÏÁö ¾ÊÀº ¼¼Æ÷ ´ëºñ % cytotoxicity·Î Á¤¸®µÇ¾ú´Ù. ±× °á°ú TransIT¢ç-LT1 Transfection Reagent´Â ³ôÀº µµÀÔÈ¿À²°ú ³·Àº µ¶¼ºÀ¸·Î Ÿ»ç ´ëºñ ³ôÀº È¿À²À» º¸¿´´Ù.


±×¸² 2. ´Ù¾çÇÑ cell type¿¡ »ç¿ë °¡´É. °¢°¢ÀÇ cell-line¿¡ 2¹Ýº¹À¸·Î 12-well plate¿¡¼­ well´ç 1 §¶ÀÇ luciferase expression vector¸¦ 3 §¡ÀÇ TransIT¢ç-LT1 ȤÀº FuGENE¢ç 6 ReagentsÀ¸·Î °¢ Á¦Á¶»çÀÇ ±ÇÀå Á¶°ÇÀ¸·Î »ç¿ëÇÑ °á°úÀÌ´Ù. ¸ðµç ¼¼Æ÷µéÀº transfection ÈÄ 24½Ã°£ µÚ harvestµÇ¾î luciferase activity¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú TransIT¢ç-LT1 Transfection Reagent´Â ´Ù¾çÇÑ cell type¿¡¼­µµ °íÈ¿À²·Î À¯ÀüÀÚ¸¦ ¹ßÇö½Ãų ¼ö ÀÖ¾ú´Ù.

Keyword : V2300,MIR2300,V2304T,MIR2304,V2305T,MIR2305,V2306T,MIR2306,V2306T,MIR2306

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