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범용적인 Transfection 시약

TransIT®-LT1 Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 2304
TransIT-LT1 Transfection Reagent
관련학술 관심상품등록 구매하기
0.4 ml
가격문의   ml001_transit_lt1.pdf
Mirus
MIR 2300
TransIT-LT1 Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml
가격문의  
Mirus
MIR 2305
TransIT-LT1 Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml×5
가격문의  
Mirus
MIR 2306
TransIT-LT1 Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml×10
가격문의  

  • 광범위한 세포에 DNA를 고효율로 도입 가능
  • 낮은 세포 독성으로 배지교환 불필요
  • Transient 또는 stable cell line 제작 가능
  • 단일 또는 복수의 plasmid 도입 가능

New Application : iPS 세포에 Reverse transfection 을 이용한 고효율 유전자 도입
Reverse Transfection of Human Induced Pluripotent Stem (iPS) Cells with TransIT®-LT1 Transfection Reagent (클릭)


Figure 1. Exceptional Transfection Efficiency in Human Induced Pluripotent Stem Cells (iPSCs) via Reverse Transfection with TransIT®-LT1. The TransIT®-LT1 Transfection Reagent was used to reverse transfect 1.3 x 106 iPS cells with a ZsGreen expressing plasmid (Clontech). Reverse transfections were performed in 6-well plates using 12 μl of  TransIT®-LT1 Transfection Reagent to deliver 4 μg of DNA (3:1, reagent: DNA). Cells were visualized 48 hours post-transfection and imaged under a 10X objective with an Olympus IX71® Inverted Microscope. Images are (A) phase contrast and (B) green fluorescence. Cells were assayed 48 hours post-transfection on an Accuri® Cytometer. The histogram (C) shows untransfected cells (black line) compared to cells transfected with plasmid using TransIT®-LT1 (green line)


Figure 2. High Efficiency Transfection of iCell® Cardiomyocytes Using TransIT®-LT1 Transfection Reagent. iCell® Cardiomyocytes were plated at 20,000 cells/well in a 96 well tissue culture plate coated with 0.1% gelatin. After allowing the cells to recover from thaw, cells were transfected with 100 ng/well of pMAXGFP (Lonza) using TransIT®-LT1 Transfection Reagent with a 2:1 reagent-to-DNA ratio according to the manufacturer’s instructions. Fluorescent images were taken 3 days post transfection using an Olympus IX71® inverted microscope.


Figure 3. The TransIT®-LT1 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. HepG2 cells were transfected with a luciferase expression plasmid using the designated reagents at the manufacturer’s recommended reagent-to-DNA ratio indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 μg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Experiments were performed as per industry accepted testing protocols. FuGENE® is a registered trademark of Fugent LLC. Lipofectamine® is a trademark of Life Technologies Corporation.

Keyword : V2300,MIR2300,V2304T,MIR2304,V2305T,MIR2305,V2306T,MIR2306,V2306T,MIR2306
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