¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
Transfection ½Ã¾à
>
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
> VirusGEN¢ç LV Transfection Kit
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Mirus
MIR 6760
VirusGEN¢ç LV Transfection Kit
1 Kit (1 L cell culture)
(Package)
°¡°Ý¹®ÀÇ
Mirus
MIR 6825-GMP
VirusGEN¢ç GMP LV Transfection Kit
1 Kit (50 L cell culture)
(Package)
°¡°Ý¹®ÀÇ
Mirus
MIR 6620
TransduceIT¢â Transduction Reagent
1 §¢
°¡°Ý¹®ÀÇ
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
*VirusGEN
®
GMP kitÀÇ °ßÀû ¹× ±¸¸Å´Â ¹Ýµå½Ã ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (T. 02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
³ôÀº °¨¿°È°¼ºÀ» °¡Áö´Â °íÇ°ÁúÀÇ Lentivirus »ý»ê¿¡ ÃÖÀû
HEK293 ¼¼Æ÷ ¹è¾ç ½Ã½ºÅÛ¿¡ ÃÖÀû (adherent ¹× suspension ¹è¾ç ¸ðµÎ Àû¿ë)
¿¬±¸¿ë ½Ã¾àºÎÅÍ ÀüÀÓ»ó ´Ü°è, ÀÓ»ó¿ë GMP grade±îÁö ¶óÀξ÷
Á¦Ç°¼³¸í
Trans
IT-VirusGEN
®
Transfection Reagent´Â expression vector¿Í packaging plasmid¸¦ HEK293 ŸÀÔÀÇ ÆÐŰ¡ ¼¼Æ÷·Î È¿À²ÀûÀ¸·Î µµÀÔÇÏ¿©, AAV ¶Ç´Â LentivirusÀÇ »ý»êÀ²À» ³ô¿©ÁÖ´Â transfection ½Ã¾àÀÌ´Ù.
±× Áß¿¡¼µµ º» VirusGEN
®
LV Transfection Kit Á¦Ç°Àº
Trans
IT-VirusGEN
®
Transfection Reagent¸¦ ±â¹ÝÀ¸·Î plasmid µµÀÔ È¿À²À» ³ôÀ̱â À§ÇÑ VirusGEN
®
LV Complex Formation Solution°ú VirusGEN
®
LV Enhancer°¡ Ãß°¡µÇ¾î LentivirusÀÇ È¿À²ÀûÀÎ »ý»êÀ» À§ÇØ ÃÖÀûÈµÈ Á¦Ç°ÀÌ´Ù.
VirusGEN
®
SELECT LV Transfection Kit´Â ÀüÀÓ»ó ¿¬±¸ ¹× °øÁ¤ °³¹ß¿¡¼ ÇÊ¿äÇÑ ´ë±Ô¸ð ¹ÙÀÌ·¯½º »ý»êÀ» À§ÇÑ ÃÖÀûÀÇ ½Ã¾àÀ¸·Î °£ÆíÇÏ°í ºñ¿ë È¿À²ÀûÀÎ ½ÇÇè ÇÁ·Î¼¼½º¸¦ Á¦°øÇÑ´Ù. ¶ÇÇÑ ÀüÀÓ»ó¿ë ¹ÙÀÌ·¯½º º¤ÅÍ Á¦ÀÛÀ» À§ÇÑ transfection ½Ã¾àÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖµµ·Ï Ç°Áú º¸Áõ ½ÃÇèÀ» ¿Ï·áÇÏ¿´´Ù.
[
Example Certificate of Origin (PDF)
|
Example Certificate of Analysis (PDF)
|
Example BSE TSE Statement (PDF)
])
µû¶ó¼, ÀÓ»ó ½ÃÇè ¶Ç´Â »ó¾÷ÀûÀÎ ¹ÙÀÌ·¯½º »ý»êÀ» À§Çؼ´Â VirusGEN
®
GMP LV Transfection Kit¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
±×¸²1. Ÿ»ç´ëºñ ³ôÀº Lentivirus functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼ ¹è¾ç)(A)¿Í Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡
Trans
IT-VirusGEN
®
Transfection Reagent (reagent : DNA (vol : wt) = 3 : 1), Ÿ»ç B4 (2:1)(A), 3:1 (B), Ÿ»ç B5 (2:2:1 (A) ¶Ç´Â 3:3:1 (B)), 25 kDa PEI (4:1, Polysciences) À¸·Î transfection ÇÏ¿´´Ù.
À̶§ pLKO.1-puro-CMV-TurboGFP™ transfer vector ¿Í Lentivirus Packaging Mix powered by MISSION
®
(1:1 ratio, 1 µg/ml)À» ÀÌ¿ëÇÏ¿´´Ù. Transfection ÈÄ ¼¼Æ÷ ¹è¾ç »óµî¾×À» ȸ¼öÇÏ¿© 0.45 µm filtering ÈÄ, 293T/17 cell¿¡ 8 µg/ml
Transduce
IT™·Î transductionÇÏ¿´´Ù. °¨¿° 72½Ã°£ ÈÄ¿¡ GFP ¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿© functional titer¸¦ È®ÀÎÇÏ¿´´Ù.
*Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.
±×¸² 2.
Trans
IT-VirusGEN
®
Reagent, VirusGEN
®
LV Transfection Kit - Ÿ»ç Á¦Ç°°úÀÇ »ý»êÈ¿À² ºñ±³
Trans
IT-ViruGEN
®
Transfection Reagent, VirusGEN
®
LV Transfection Kit, AÁ¦Ç° 3, CÁ¦Ç° 1À» ÀÌ¿ëÇÏ¿© lentivirusÀÇ »ý»ê ´É·ÂÀ» ºñ±³ÇÏ¿´´Ù. Expi293 Expression Media (Thermo Fisher Scientific)¿¡¼ ¹è¾çÇÑ Expi293F cells (Thermo Fisher Scientific)¿¡ plasmid set (A. pALD-Lenti EGFP-A transfer vector, pALD-VSV-G-A, pALD-Rev-A¿Í pALD-GagPol-A packaging vectors (DNAºñÀ² 3:0.5:0.5:2, Aldevron), B. pLKO.1-puro-CMV-TurboGFP transfer vector (Millipore Sigma), ViraSafe Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, CellBio Lab) DNAºñÀ² 3:0.5:0.5:2)À» À¯ÀüÀÚ µµÀÔÇÏ°í ¹ÙÀÌ·¯½º¸¦ ȸ¼öÇÏ¿´´Ù. ȸ¼öÇÑ ¹ÙÀÌ·¯½º¸¦ 293T/17¼¼Æ÷¿¡ °¨¿°½ÃÅ°°í 72½Ã°£ ÈÄ¿¡ GFPÀÇ ¹ßÇöÀ» È®ÀÎÇÏ¿© ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿´´Ù.
±× °á°ú,
Trans
IT-VirusGEN
®
Reagent, VirusGEN
®
LV Transfection Kit¸¦ ÀÌ¿ëÇßÀ» ¶§ ´õ ¸¹Àº ¿ª°¡ÀÇ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú°í, ƯÈ÷ VirusGEN
®
LV Transfection Kit¸¦ ÀÌ¿ëÇÏ¿´À» ¶§ °¡Àå ¸¹Àº ¾çÀÇ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú´Ù.
º¸Á¸
Trans
IT-VirusGEN
®
Transfection Reagent : -10~-30¡É º¸Á¸
VirusGEN
®
LV Enhancer : 2~10¡É
VirusGEN
®
LV Complex Formation Solution : 2~10¡É
(SELECT, GMP ¸ðµÎ µ¿ÀÏ)
°ü·ÃÁ¦Ç°
- Tat-independent Lentivirus system
:
LVpro Packaging Mix (pLVpro-MSCV Vector)
:
LVpro Packaging Mix (pLVpro-EF1α Vector)
:
LVpro Packaging Mix (pLVpro-MSCV-ZsGreen1 Vector)
- Mix & Spin ÃÊ°£´Ü ³óÃà:
Lenti-X™ Concentrator
- °íÈ¿À² °¨¿°À» À§ÇÑ Lentivirus transduction enhancer
:
RetroNectin® (Recombinant Human Fibronectin Fragment)
:
RetroNectin® GMP grade (Recombinant Human Fibronectin Fragment CH-296)
- ÃÖÀûÀÇ Lentivirus »ý»êÀ» À§ÇÑ Á¦Ç° ¼±Åð¡À̵å (
¸µÅ©
)
Keyword :
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
Trans
IT-VirusGEN¢ç Transfection Reagent
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
Trans
IT¢ç-Lenti Transfection Reagent
Trans
IT-PRO¢ç Transfection Reagent & Kit
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit