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Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)

VirusGEN¢ç LV Transfection Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 6760
VirusGEN¢ç LV Transfection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit (1 L cell culture)
(Package)
°¡°Ý¹®ÀÇ   virusgen-lv-transfection-kit-protocol.pdf
Mirus
MIR 6825-GMP
VirusGEN¢ç GMP LV Transfection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit (50 L cell culture)
(Package)
°¡°Ý¹®ÀÇ   virusgen-gmp-lv-transfection-kit-protocol.pdf
Mirus
MIR 6620
TransduceIT¢â Transduction Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 §¢
°¡°Ý¹®ÀÇ   transduceit-reagent-pds.pdf

*VirusGEN® GMP kitÀÇ °ßÀû ¹× ±¸¸Å´Â ¹Ýµå½Ã ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (T. 02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
  • ³ôÀº °¨¿°È°¼ºÀ» °¡Áö´Â °íÇ°ÁúÀÇ Lentivirus »ý»ê¿¡ ÃÖÀû
  • HEK293 ¼¼Æ÷ ¹è¾ç ½Ã½ºÅÛ¿¡ ÃÖÀû (adherent ¹× suspension ¹è¾ç ¸ðµÎ Àû¿ë)
  • ¿¬±¸¿ë ½Ã¾àºÎÅÍ ÀüÀÓ»ó ´Ü°è, ÀÓ»ó¿ë GMP grade±îÁö ¶óÀξ÷
Á¦Ç°¼³¸í
TransIT-VirusGEN® Transfection Reagent´Â expression vector¿Í packaging plasmid¸¦ HEK293 ŸÀÔÀÇ ÆÐŰ¡ ¼¼Æ÷·Î È¿À²ÀûÀ¸·Î µµÀÔÇÏ¿©, AAV ¶Ç´Â LentivirusÀÇ »ý»êÀ²À» ³ô¿©ÁÖ´Â transfection ½Ã¾àÀÌ´Ù.
±× Áß¿¡¼­µµ º» VirusGEN® LV Transfection Kit Á¦Ç°Àº TransIT-VirusGEN® Transfection Reagent¸¦ ±â¹ÝÀ¸·Î plasmid µµÀÔ È¿À²À» ³ôÀ̱â À§ÇÑ VirusGEN® LV Complex Formation Solution°ú VirusGEN® LV Enhancer°¡ Ãß°¡µÇ¾î LentivirusÀÇ È¿À²ÀûÀÎ »ý»êÀ» À§ÇØ ÃÖÀûÈ­µÈ Á¦Ç°ÀÌ´Ù.
VirusGEN® SELECT LV Transfection Kit´Â ÀüÀÓ»ó ¿¬±¸ ¹× °øÁ¤ °³¹ß¿¡¼­ ÇÊ¿äÇÑ ´ë±Ô¸ð ¹ÙÀÌ·¯½º »ý»êÀ» À§ÇÑ ÃÖÀûÀÇ ½Ã¾àÀ¸·Î °£ÆíÇÏ°í ºñ¿ë È¿À²ÀûÀÎ ½ÇÇè ÇÁ·Î¼¼½º¸¦ Á¦°øÇÑ´Ù. ¶ÇÇÑ ÀüÀÓ»ó¿ë ¹ÙÀÌ·¯½º º¤ÅÍ Á¦ÀÛÀ» À§ÇÑ transfection ½Ã¾àÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖµµ·Ï Ç°Áú º¸Áõ ½ÃÇèÀ» ¿Ï·áÇÏ¿´´Ù.
[Example Certificate of Origin (PDF) | Example Certificate of Analysis (PDF) | Example BSE TSE Statement (PDF)])
µû¶ó¼­, ÀÓ»ó ½ÃÇè ¶Ç´Â »ó¾÷ÀûÀÎ ¹ÙÀÌ·¯½º »ý»êÀ» À§Çؼ­´Â VirusGEN® GMP LV Transfection Kit¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Ù.


±×¸²1. Ÿ»ç´ëºñ ³ôÀº Lentivirus functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼­ ¹è¾ç)(A)¿Í Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡ Trans IT-VirusGEN® Transfection Reagent (reagent : DNA (vol : wt) = 3 : 1), Ÿ»ç B4 (2:1)(A), 3:1 (B), Ÿ»ç B5 (2:2:1 (A) ¶Ç´Â 3:3:1 (B)), 25 kDa PEI (4:1, Polysciences) À¸·Î transfection ÇÏ¿´´Ù.
À̶§ pLKO.1-puro-CMV-TurboGFP™ transfer vector ¿Í Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 1 µg/ml)À» ÀÌ¿ëÇÏ¿´´Ù. Transfection ÈÄ ¼¼Æ÷ ¹è¾ç »óµî¾×À» ȸ¼öÇÏ¿© 0.45 µm filtering ÈÄ, 293T/17 cell¿¡ 8 µg/ml TransduceIT™·Î transductionÇÏ¿´´Ù. °¨¿° 72½Ã°£ ÈÄ¿¡ GFP ¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿© functional titer¸¦ È®ÀÎÇÏ¿´´Ù.
*Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.


±×¸² 2. TransIT-VirusGEN® Reagent, VirusGEN® LV Transfection Kit - Ÿ»ç Á¦Ç°°úÀÇ »ý»êÈ¿À² ºñ±³
TransIT-ViruGEN® Transfection Reagent, VirusGEN® LV Transfection Kit, AÁ¦Ç° 3, CÁ¦Ç° 1À» ÀÌ¿ëÇÏ¿© lentivirusÀÇ »ý»ê ´É·ÂÀ» ºñ±³ÇÏ¿´´Ù. Expi293 Expression Media (Thermo Fisher Scientific)¿¡¼­ ¹è¾çÇÑ Expi293F cells (Thermo Fisher Scientific)¿¡ plasmid set (A. pALD-Lenti EGFP-A transfer vector, pALD-VSV-G-A, pALD-Rev-A¿Í pALD-GagPol-A packaging vectors (DNAºñÀ² 3:0.5:0.5:2, Aldevron), B. pLKO.1-puro-CMV-TurboGFP transfer vector (Millipore Sigma), ViraSafe Pantropic Packaging mix (pRSV-Rev, pCMV-VSV-G, pCgpV, CellBio Lab) DNAºñÀ² 3:0.5:0.5:2)À» À¯ÀüÀÚ µµÀÔÇÏ°í ¹ÙÀÌ·¯½º¸¦ ȸ¼öÇÏ¿´´Ù. ȸ¼öÇÑ ¹ÙÀÌ·¯½º¸¦ 293T/17¼¼Æ÷¿¡ °¨¿°½ÃÅ°°í 72½Ã°£ ÈÄ¿¡ GFPÀÇ ¹ßÇöÀ» È®ÀÎÇÏ¿© ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿´´Ù.
±× °á°ú, TransIT-VirusGEN® Reagent, VirusGEN® LV Transfection Kit¸¦ ÀÌ¿ëÇßÀ» ¶§ ´õ ¸¹Àº ¿ª°¡ÀÇ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú°í, ƯÈ÷ VirusGEN® LV Transfection Kit¸¦ ÀÌ¿ëÇÏ¿´À» ¶§ °¡Àå ¸¹Àº ¾çÀÇ lentivirus¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú´Ù.
º¸Á¸
TransIT-VirusGEN® Transfection Reagent : -10~-30¡É º¸Á¸
VirusGEN® LV Enhancer : 2~10¡É
VirusGEN® LV Complex Formation Solution : 2~10¡É
(SELECT, GMP ¸ðµÎ µ¿ÀÏ)
°ü·ÃÁ¦Ç°
- Tat-independent Lentivirus system
   : LVpro Packaging Mix (pLVpro-MSCV Vector)
   : LVpro Packaging Mix (pLVpro-EF1α Vector)
   : LVpro Packaging Mix (pLVpro-MSCV-ZsGreen1 Vector)
- Mix & Spin ÃÊ°£´Ü ³óÃà: Lenti-X™ Concentrator
- °íÈ¿À² °¨¿°À» À§ÇÑ Lentivirus transduction enhancer
   : RetroNectin® (Recombinant Human Fibronectin Fragment)
   : RetroNectin® GMP grade (Recombinant Human Fibronectin Fragment CH-296)
- ÃÖÀûÀÇ Lentivirus »ý»êÀ» À§ÇÑ Á¦Ç° ¼±Åð¡À̵å (¸µÅ©)

Keyword :

È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
>
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
TransIT-VirusGEN¢ç Transfection Reagent
>
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
TransIT¢ç-Lenti Transfection Reagent
>
AAV »ý»ê·®À» ³ôÀÌ´Â transfection enhancer
RevIT¢â AAV Enhancer
>
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
>
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit