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TaKaRa
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Àú³óµµ DTT¿¡¼­µµ ¾ÈÁ¤ÀûÀÎ RNase inhibitor

Recombinant RNase Inhibitor ver.2.0

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2315A
Recombinant RNase Inhibitor ver.2.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
5,000 U
104,800¿ø 
131,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2315A_DS.v2210Da.pdf
Takara
2315B
Recombinant RNase Inhibitor ver.2.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
25,000 U (2315A x 5)
595,000¿ø 

  • µÅÁö °£ À¯·¡ÀÇ recombinant RNase inhibitor
  • ÀçÁ¶ÇÕ ´Ü¹éÁúÀÇ Cys Àܱ⿡ º¯À̸¦ µµÀÔÇÏ¿© »êÈ­ ¹ÝÀÀ¿¡ ´ëÇÑ ÀúÇ×¼º °­È­ (Àú³óµµ DTT Àû¿ë °¡´É)
  • RNA ¾ÈÁ¤¼ºÀÌ ¿ä±¸µÇ´Â in vitro transcription ¹× RT-PCR µî ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´É
Á¦Ç°¼³¸í
Recombinant RNase Inhibitor ver.2.0Àº µÅÁö °£ À¯·¡ÀÇ Recombinant RNase Inhibitor (Code 2313A)ÀÇ ¾÷±×·¹À̵å Á¦Ç°ÀÌ´Ù. ÀÌ Á¦Ç°Àº Àΰ£ Å¹ÝÀ̳ª µÅÁö °£¿¡¼­ Á÷Á¢ Á¤Á¦ÇÑ RNase inhibitor¿Í ¸Å¿ì ºñ½ÁÇÑ ¼ºÁúÀ» À¯ÁöÇϸ鼭, ºÒÈ°¼ºÈ­ÀÇ ¿øÀÎÀÌ µÇ±â ½¬¿î Cys Àܱ⿡ º¯À̸¦ µµÀÔÇØ »êÈ­ ¹ÝÀÀ¿¡ ´ëÇÑ ÀúÇ×¼ºÀ» ¿Ã·Á ´Ü¹éÁúÀÇ ¾ÈÁ¤¼ºÀ» °­È­ÇÏ¿´´Ù (±×¸² 1).
RNase Inhibitor´Â RNaseA¿Í 1:1ÀÇ º¹ÇÕü¸¦ Çü¼ºÇÏ¿© RNaseÀÇ È°¼ºÀ» ¾ïÁ¦ÇÑ´Ù. ÀÌ·¯ÇÑ ¹ÝÀÀÀº °¡¿ªÀûÀ̹ǷÎ, urea ¶Ç´Â sulfhydryl ½Ã¾àÀ¸·Î º¹ÇÕü¸¦ Çظ® ½ÃÅ´À¸·Î½á RNaseÀÇ È°¼ºÀ» µÇ»ì¸®°í, inhibitor¸¦ ºñ°¡¿ªÀûÀ¸·Î ½ÇÈ° ½Ãų ¼ö ÀÖ´Ù. ¶ÇÇÑ, ±âÁ¸ÀÇ ±æÇ×¼º ÀúÇØÁ¦ (nucleotide·ù, ¹«±âÀλê·ù)¿Í´Â ´Þ¸® ´Ü¹éÁúÀ̹ǷΠÆä³î 󸮷Π¹ÝÀÀ°è¿¡¼­ °£´ÜÈ÷ Á¦°ÅÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ RNase HÀÇ È°¼ºÀº ÀúÇØÇÏÁö ¾Ê´Â´Ù.
º» Á¦Ç°Àº RNAÀÇ ¾ÈÁ¤¼ºÀÌ Áß¿äÇÑ in vitro transcription ¹× RT-PCRµî ´Ù¾çÇÑ ½ÇÇè¿¡ »ç¿ëµÉ ¼ö ÀÖ´Ù.

±×¸² 1. »êÈ­ ¹ÝÀÀ¿¡ ´ëÇÑ ÀúÇ×¼º ºñ±³ ½ÇÇè °á°ú
»êÈ­ ¹ÝÀÀ¿¡ ´ëÇÑ ÀúÇ×¼ºÀÌ °­È­µÈ Recombinant RNase Inhibitor ver.2.0 (Code 2315A)¿Í ±âÁ¸ Á¦Ç°ÀÎ Recombinant RNase Inhibitor (Code 2313A) ¹× Ÿ»çÀÇ RNase inhibitor, ÃÑ 3Á¾ÀÇ Á¦Ç°À» °ú»êÈ­¼ö¼Ò H202 ³óµµ¸¦ Áõ°¡½ÃÅ°¸é¼­ »óÃþ¾×À¸·Î ¹æÃâµÈ ÇÙ»êÀÇ ¾ç (RNaseA-mediated rRNA ºÐÇØ ÁöÇ¥)À» ÃøÁ¤ÇÏ¿´´Ù (3¹Ýº¹ 2ȸ ÃøÁ¤). H202ÀÇ ³óµµ°¡ ³·Àº °æ¿ì¿¡´Â È°¼ºÀÇ Â÷ÀÌ°¡ Å©Áö ¾ÊÁö¸¸, 0.01% ÀÌ»óÀÇ °æ¿ì¿¡´Â È¿¼ÒÀÇ È°¼ºÀÌ Â÷À̸¦ º¸À̱⠽ÃÀÛÇÏ¿´´Ù. ±× °á°ú, È¿¼ÒÀÇ ºÒÈ°¼ºÈ­ÀÇ ÁÖ¿ä ¿äÀÎÀ¸·Î »ý°¢µÇ´Â Cys Àܱ⿡ º¯À̸¦ µµÀÔÇÑ Recombinant RNase Inhibitor ver.2.0 (Code 2315A)ÀÌ °¡Àå °­ÇÑ »êÈ­ ¹ÝÀÀ¿¡ ´ëÇÑ ÀúÇ×¼ºÀ» °¡Áø °ÍÀÌ È®ÀεǾú´Ù.
¿ëµµ
- in vitro transcription/translation (1 U/§¡ reaction)*
- in vitro transcription/translation with cell-free extract (20 U/§¡ reaction)*
- RT-PCR (0.5 U/§¡ reaction)*
- cDNA synthesis (0.5 U/§¡ reaction)*
- Polysome isolation (1 U/§¡ reaction)*
*°¢ ¹ÝÀÀ¾×¿¡¼­ÀÇ RNase inhibitor »ç¿ë ³óµµ ¿¹½Ã
³»¿ë

Recombinant RNase Inhibitor ver.2.0 (40 U/§¡)

 5,000 U

º¸Á¸ -20¡É
Çü»ó

HEPES-KOH, pH7.5

20 mM

KCl

50 mM

DTT

5 mM

Glycerol

50%

±â¿ø
E. coli carrying the plasmid containing the gene for ribonuclease inhibitor from porcine liver
ÀϹÝÀûÀÎ ¼ºÁú
- ºÐÀÚ·®: ¾à 52 kDa
- ÃÖÀû pH: RNase ÀúÇØ È°¼ºÀº ´Ù¾çÇÑ pH¿¡¼­ º¸ÀÌÁö¸¸, pH7~8¿¡¼­ ÃÖ´ë È°¼ºÀ» º¸ÀδÙ.
È°¼ºÀÇ Á¤ÀÇ
5 ngÀÇ RNase A È°¼ºÀ» 50% ÀúÇØÇÏ´Â È¿¼Ò·®À» 1UÀ¸·Î ÇÑ´Ù.
(2', 3'-cyclic CMP¿¡¼­ RNase A¿¡ ÀÇÇØ »ý¼ºµÇ´Â 3'-CMP¸¦ Á¤·®)
»ç¿ë»óÀÇ ÁÖÀÇ
- º» È¿¼ÒÀÇ °úµµÇÑ vortexingÀº ÇÇÇϽñ⠹ٶø´Ï´Ù.
- È°¼º À¯Áö¸¦ À§ÇÏ¿© ¸ñÀûÇÏ´Â ½ÇÇèÀÇ ¹ÝÀÀÀ» ÀúÇØÇÏÁö ¾Ê´Â ³óµµÀÇ DTT¸¦ ¹ÝÀÀ¾×¿¡ ÷°¡ÇÏ¿© ½ÇÇèÀ» ÁøÇàÇÑ´Ù (e.g. 1 mM).
°ü·ÃÁ¦Ç°
- Çѹø¿¡ 200 §¶ mRNA ÇÕ¼º, °í¼öÀ² in vitro transcription: Takara IVTpro™ T7 mRNA Synthesis Kit (Code 6144)
- uncapped RNAÀÇ 5’ ¸»´Ü¿¡ Cap 0 ±¸Á¶¸¦ ºÎ¿©ÇÏ´Â È¿¼Ò Vaccinia Capping Enzyme (Code 2460A)
- Cap 0 ±¸Á¶¸¦ °¡Áø RNA¸¦ Cap 1À¸·Î ¸¸µå´Â È¿¼Ò mRNA Cap 2'-O-Methyltransferase (Code 2470A)
- mRNA Àü¿ë Transfection ½Ã¾à, TransIT®-mRNA Transfection Kit (Code MIR 2250)
- IVT¿ë ÁÖÇü Á¦ÀÛ¿¡ ÃÖÀûÀÎ Type IIS Á¦ÇÑÈ¿¼Ò Bsp QI (Code 1227A)
- RNA ÇÕ¼º ¼öÀ²ÀÌ Çâ»óµÈ Ver.2 T7 RNA Polymerase ver.2.0 (Code 2541A)
- RNA, DNA ÇÕ¼º ½Ã PPi¸¦ Á¦°ÅÇÏ¿© ¼öÀ²À» ³ôÀ̴ Pyrophosphatase (inorganic) (Code 2450A)

Keyword :

in vitro transcription
>
°íÇ°Áú °í¼öÀ² in vitro transcriptionÀ» À§ÇÑ ..
Takara IVTpro¢â mRNA Synthesis System
>
dsRNA »ý¼ºÀÌ Àû¾î ÀÓ»óºÐ¾ßÀÇ ¿¬±¸°³¹ß¿¡ ÀûÇÕ..
PrimeCap¢â T7 RNA Polymerase (low dsRNA)
>
Poly(A) ÀÌÈÄ Ãß°¡¼­¿­ÀÌ ¾ø´Â scarless mRNA ..
Template Vector (BspQ I) for T7 mRNA Synthesis
>
RNAÀÇ 5¡¯-¸»´Ü¿¡ Cap (Cap 0)À» ºÎ°¡ÇÏ´Â È¿¼Ò
Vaccinia Capping Enzyme
>
RNAÀÇ 5¡¯ ¸»´ÜÀ» Cap 0 ¡æ Cap 1·Î º¯È¯ÇÏ´Â ..
mRNA Cap 2-O-Methyltransferase
>
´Ù¾çÇÑ ¿Âµµ¿¡¼­ ¹ÝÀÀÇÒ ¼ö ÀÖ´Â Capping enzym..
Faustovirus Capping Enzyme (S17)
>
Poly(A) ¹× ¹Ýº¹ ¼­¿­À» Æ÷ÇÔÇÏ´Â À¯ÀüÀÚÀÇ Å¬..
Takara Stable Competent Cells
>
RNA ÇÕ¼º ¼öÀ²ÀÌ Çâ»óµÈ
T7 RNA Polymerase ver.2.0
>
Àú³óµµ DTT¿¡¼­µµ ¾ÈÁ¤ÀûÀÎ RNase inhibitor
Recombinant RNase Inhibitor ver.2.0
>
DNA, RNA ÇÕ¼º ¹ÝÀÀÀ» ÃËÁø
Pyrophosphatase (inorganic)
>
IVT Àû¿ë¿¡ ÃÖÀû, Type IIS Á¦ÇÑÈ¿¼Ò
BspQ I
>
´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ
0.5-10 kb ssRNA Ladder Marker